EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES
The possible side effects of drugs used in type II diabetes are increasing the tendency to herbal resources that have been used for many years. Senecio vernalis Waldst. amp; Kit is one of the annual Senecio L. species widely distributed in Turkey and used as a food and folk medicine. In this study,...
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| Format: | Article |
| Language: | English |
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Trakya University
2021-10-01
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| Series: | Trakya University Journal of Natural Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/1852081 |
| _version_ | 1797633855381831680 |
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| author | Nurcan Doğan Cemhan Doğan |
| author_facet | Nurcan Doğan Cemhan Doğan |
| author_sort | Nurcan Doğan |
| collection | DOAJ |
| description | The possible side effects of drugs used in type II diabetes are increasing the tendency to herbal resources that have been used for many years. Senecio vernalis Waldst. amp; Kit is one of the annual Senecio L. species widely distributed in Turkey and used as a food and folk medicine. In this study, optimization of extraction conditions on the bioactive properties (Total phenolic content (TPC) and antioxidant capacity) of the flowers of S. vernalis and the potential of the plant for α-amylase, α-glucosidase, and lipase inhibitory activity were investigated. The optimum extraction conditions were determined at 69.72% water concentration, 59℃ for 26.15 min, and the highest experimental values of TPC and 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) scavenging activity were observed as 28.14 mg gallic acid equivalent (GAE) g-1 and 3165.99 mg trolox equivalent (TE)/100 g sample, respectively. Significant inhibition was observed for α-amylase and α-glucosidase which are the key enzymes in type II diabetes, at a concentration of 100 mg mL-1, with 21.32% and 64.16% respectively. The S. vernalis extracts showed no detectable inhibition of lipase. The results showed that S. vernalis, which has high antioxidant capacity also has a significant anti-diabetic effect. It can be concluded that S. vernalis can be considered a natural resource in many industries such as food and pharmaceuticals. |
| first_indexed | 2024-03-11T12:00:30Z |
| format | Article |
| id | doaj.art-2a2e42653d8d42c2aa3eeadc2141ec44 |
| institution | Directory Open Access Journal |
| issn | 2528-9691 |
| language | English |
| last_indexed | 2024-03-11T12:00:30Z |
| publishDate | 2021-10-01 |
| publisher | Trakya University |
| record_format | Article |
| series | Trakya University Journal of Natural Sciences |
| spelling | doaj.art-2a2e42653d8d42c2aa3eeadc2141ec442023-11-08T07:27:55ZengTrakya UniversityTrakya University Journal of Natural Sciences2528-96912021-10-0122224525310.23902/trkjnat.96007369EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIESNurcan Doğan0Cemhan Doğan1Yozgat Bozok Üniversitesi Boğazlıyan Meslek Yüksekokulu Gıda İşleme BölümüYOZGAT BOZOK ÜNİVERSİTESİ, BOĞAZLIYAN MESLEK YÜKSEKOKULU, GIDA İŞLEME BÖLÜMÜThe possible side effects of drugs used in type II diabetes are increasing the tendency to herbal resources that have been used for many years. Senecio vernalis Waldst. amp; Kit is one of the annual Senecio L. species widely distributed in Turkey and used as a food and folk medicine. In this study, optimization of extraction conditions on the bioactive properties (Total phenolic content (TPC) and antioxidant capacity) of the flowers of S. vernalis and the potential of the plant for α-amylase, α-glucosidase, and lipase inhibitory activity were investigated. The optimum extraction conditions were determined at 69.72% water concentration, 59℃ for 26.15 min, and the highest experimental values of TPC and 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) scavenging activity were observed as 28.14 mg gallic acid equivalent (GAE) g-1 and 3165.99 mg trolox equivalent (TE)/100 g sample, respectively. Significant inhibition was observed for α-amylase and α-glucosidase which are the key enzymes in type II diabetes, at a concentration of 100 mg mL-1, with 21.32% and 64.16% respectively. The S. vernalis extracts showed no detectable inhibition of lipase. The results showed that S. vernalis, which has high antioxidant capacity also has a significant anti-diabetic effect. It can be concluded that S. vernalis can be considered a natural resource in many industries such as food and pharmaceuticals.https://dergipark.org.tr/en/download/article-file/1852081senecio vernalistype ii diabetesantioxidant activityextraction optimization |
| spellingShingle | Nurcan Doğan Cemhan Doğan EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES Trakya University Journal of Natural Sciences senecio vernalis type ii diabetes antioxidant activity extraction optimization |
| title | EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES |
| title_full | EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES |
| title_fullStr | EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES |
| title_full_unstemmed | EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES |
| title_short | EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES |
| title_sort | extraction optimization of senecio vernalis waldst kit and determination of anti α amylase α glucosidase anti lipase and antioxidant activities |
| topic | senecio vernalis type ii diabetes antioxidant activity extraction optimization |
| url | https://dergipark.org.tr/en/download/article-file/1852081 |
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