Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics
Background: Verbascoside (VB), as an active component of multiple medicinal plants, has been proved to exert anti-oxidative, anti-aging and neuroprotective effects. This study was designed to investigate whether VB could play a cardioprotective role in septic heart injury. Methods: Mice were injecte...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322001671 |
| _version_ | 1819349567079972864 |
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| author | Xuanfeng Zhu Min Sun Hongmei Guo Gan Lu Jianhua Gu Lingling Zhang Licheng Shi Jia Gao Dandan Zhang Wenjun Wang Jiannan Liu Xia Wang |
| author_facet | Xuanfeng Zhu Min Sun Hongmei Guo Gan Lu Jianhua Gu Lingling Zhang Licheng Shi Jia Gao Dandan Zhang Wenjun Wang Jiannan Liu Xia Wang |
| author_sort | Xuanfeng Zhu |
| collection | DOAJ |
| description | Background: Verbascoside (VB), as an active component of multiple medicinal plants, has been proved to exert anti-oxidative, anti-aging and neuroprotective effects. This study was designed to investigate whether VB could play a cardioprotective role in septic heart injury. Methods: Mice were injected with lipopolysaccharide (LPS; 10 mg/kg) to induce sepsis. The treatment group received an intraperitoneally injection of VB (20 mg/kg) before LPS challenge. Transthoracic echocardiography, ELISA, immunofluorescence, and qPCR were performed to assess the effect of VB on heart function, oxidative stress, inflammation and apoptosis. Transmission electronic microscopy and immunoblotting were used to evaluate the mitochondrial morphology and biogenesis of the septic heart. In vitro experiments were also performed to repeat above-mentioned assays. Results: Compared with LPS group, the VB treatment group showed improved cardiac function in sepsis. VB alleviated oxidative stress and inflammatory cell infiltration, as well as cardiomyocyte apoptosis. Specifically, VB could restore sepsis-induced mitochondrial alterations via regulating mitochondrial biogenesis. These results were also confirmed in in vitro experiments. Conclusion: Verbascoside could protected from sepsis-induced cardiomyopathy by inhibiting oxidative stress, inflammation, and apoptosis, as well as promoting mitochondrial biogenesis. |
| first_indexed | 2024-12-24T19:02:34Z |
| format | Article |
| id | doaj.art-2a30220d68a5438685b8a3e08d3fa25a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-24T19:02:34Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2a30220d68a5438685b8a3e08d3fa25a2022-12-21T16:43:10ZengElsevierEcotoxicology and Environmental Safety0147-65132022-03-01233113327Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamicsXuanfeng Zhu0Min Sun1Hongmei Guo2Gan Lu3Jianhua Gu4Lingling Zhang5Licheng Shi6Jia Gao7Dandan Zhang8Wenjun Wang9Jiannan Liu10Xia Wang11Department of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaHypertension Research Institute of Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, ChinaDepartment of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University; Jiangsu Province Official Hospital, Nanjing, China; Correspondence to: Department of Respiratory Medicine, Geriatric Hospital of Nanjing Medical University, 65 Jiangsu Road, Nanjing 210003, China.Department of Geriatric Cardiology, Taian City Central Hospital, Taian, China; Correspondence to: Department of Geriatric Cardiology, Tai’an Central Hospital, 29 Longtan Road, Tai’an 271000, China.Background: Verbascoside (VB), as an active component of multiple medicinal plants, has been proved to exert anti-oxidative, anti-aging and neuroprotective effects. This study was designed to investigate whether VB could play a cardioprotective role in septic heart injury. Methods: Mice were injected with lipopolysaccharide (LPS; 10 mg/kg) to induce sepsis. The treatment group received an intraperitoneally injection of VB (20 mg/kg) before LPS challenge. Transthoracic echocardiography, ELISA, immunofluorescence, and qPCR were performed to assess the effect of VB on heart function, oxidative stress, inflammation and apoptosis. Transmission electronic microscopy and immunoblotting were used to evaluate the mitochondrial morphology and biogenesis of the septic heart. In vitro experiments were also performed to repeat above-mentioned assays. Results: Compared with LPS group, the VB treatment group showed improved cardiac function in sepsis. VB alleviated oxidative stress and inflammatory cell infiltration, as well as cardiomyocyte apoptosis. Specifically, VB could restore sepsis-induced mitochondrial alterations via regulating mitochondrial biogenesis. These results were also confirmed in in vitro experiments. Conclusion: Verbascoside could protected from sepsis-induced cardiomyopathy by inhibiting oxidative stress, inflammation, and apoptosis, as well as promoting mitochondrial biogenesis.http://www.sciencedirect.com/science/article/pii/S0147651322001671VerbascosideSepsis-induced cardiomyopathyMitochondrial biogenesis |
| spellingShingle | Xuanfeng Zhu Min Sun Hongmei Guo Gan Lu Jianhua Gu Lingling Zhang Licheng Shi Jia Gao Dandan Zhang Wenjun Wang Jiannan Liu Xia Wang Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics Ecotoxicology and Environmental Safety Verbascoside Sepsis-induced cardiomyopathy Mitochondrial biogenesis |
| title | Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics |
| title_full | Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics |
| title_fullStr | Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics |
| title_full_unstemmed | Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics |
| title_short | Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation, oxidative stress and regulating mitochondrial dynamics |
| title_sort | verbascoside protects from lps induced septic cardiomyopathy via alleviating cardiac inflammation oxidative stress and regulating mitochondrial dynamics |
| topic | Verbascoside Sepsis-induced cardiomyopathy Mitochondrial biogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322001671 |
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