New methods for detecting and enumerating fungal spores of plant pathogens
Information on the presence or absence of airborne spores or other particles would be useful in an increasing number of areas including agriculture. Traditional methods used for detecting and enumerating of airborne spores of fungal plant pathogens are time consuming and require specialist knowledge...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200210-0024_new-methods-for-detecting-and-enumerating-fungal-spores-of-plant-pathogens.php |
| _version_ | 1797898614817685504 |
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| author | R. Kennedy A.J. Wakeham |
| author_facet | R. Kennedy A.J. Wakeham |
| author_sort | R. Kennedy |
| collection | DOAJ |
| description | Information on the presence or absence of airborne spores or other particles would be useful in an increasing number of areas including agriculture. Traditional methods used for detecting and enumerating of airborne spores of fungal plant pathogens are time consuming and require specialist knowledge. Some spore types (e.g. ascospores) are difficult to differentiate using these methods. To facilitate this, new methods, which can be used to accurately differentiate fungal spore types, are required. A Burkard 7-day volumetric spore trap used in combination with an immunofluorescence test has been developed to detect and quantify field-trapped ascosporic inoculum of Mycosphaerella brassicicola (the ringspot pathogen of brassicas). This test has also been found useful in the validation of more rapid user-friendly immunoassay based trapping procedures. A microtiter immunospore trapping device, which uses a suction system to directly trap air-particulates by impaction into microtiter wells, has been used successfully for the rapid detection and quantification of ascosporic inoculum of M. brassicicola. The system shows potential for the rapid field-detection of airborne ascosporic inoculum of the ringspot pathogen. |
| first_indexed | 2024-04-10T08:17:04Z |
| format | Article |
| id | doaj.art-2a30463c277045359cf37837e988cad9 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:17:04Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-2a30463c277045359cf37837e988cad92023-02-23T03:43:39ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-06-0138SI 1 - 6th Conf EFPPS38S4210.17221/10317-PPSpps-200210-0024New methods for detecting and enumerating fungal spores of plant pathogensR. Kennedy0A.J. Wakeham1Department of Plant Pathology, Horticulture Research International, Wellesbourne, Warwick, Warwickshire CV35 9EF, United Kingdom * Tel. +44 1789 470 385, Fax +44 1789 470 552, E-mail: roy.kennedy@hri.ac.ukDepartment of Plant Pathology, Horticulture Research International, Wellesbourne, Warwick, Warwickshire CV35 9EF, United Kingdom * Tel. +44 1789 470 385, Fax +44 1789 470 552, E-mail: roy.kennedy@hri.ac.ukInformation on the presence or absence of airborne spores or other particles would be useful in an increasing number of areas including agriculture. Traditional methods used for detecting and enumerating of airborne spores of fungal plant pathogens are time consuming and require specialist knowledge. Some spore types (e.g. ascospores) are difficult to differentiate using these methods. To facilitate this, new methods, which can be used to accurately differentiate fungal spore types, are required. A Burkard 7-day volumetric spore trap used in combination with an immunofluorescence test has been developed to detect and quantify field-trapped ascosporic inoculum of Mycosphaerella brassicicola (the ringspot pathogen of brassicas). This test has also been found useful in the validation of more rapid user-friendly immunoassay based trapping procedures. A microtiter immunospore trapping device, which uses a suction system to directly trap air-particulates by impaction into microtiter wells, has been used successfully for the rapid detection and quantification of ascosporic inoculum of M. brassicicola. The system shows potential for the rapid field-detection of airborne ascosporic inoculum of the ringspot pathogen.https://pps.agriculturejournals.cz/artkey/pps-200210-0024_new-methods-for-detecting-and-enumerating-fungal-spores-of-plant-pathogens.phpmycosphaerella brassicicolaringspotascosporesdetection |
| spellingShingle | R. Kennedy A.J. Wakeham New methods for detecting and enumerating fungal spores of plant pathogens Plant Protection Science mycosphaerella brassicicola ringspot ascospores detection |
| title | New methods for detecting and enumerating fungal spores of plant pathogens |
| title_full | New methods for detecting and enumerating fungal spores of plant pathogens |
| title_fullStr | New methods for detecting and enumerating fungal spores of plant pathogens |
| title_full_unstemmed | New methods for detecting and enumerating fungal spores of plant pathogens |
| title_short | New methods for detecting and enumerating fungal spores of plant pathogens |
| title_sort | new methods for detecting and enumerating fungal spores of plant pathogens |
| topic | mycosphaerella brassicicola ringspot ascospores detection |
| url | https://pps.agriculturejournals.cz/artkey/pps-200210-0024_new-methods-for-detecting-and-enumerating-fungal-spores-of-plant-pathogens.php |
| work_keys_str_mv | AT rkennedy newmethodsfordetectingandenumeratingfungalsporesofplantpathogens AT ajwakeham newmethodsfordetectingandenumeratingfungalsporesofplantpathogens |