Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate
The roles of the healthy microbiome on the host and the relationships between members of the microbiome remain to be fully characterized. Due to the complexity of the interactions between the mammalian microbiome and its host, the use of model organisms such as the nematode worm <i>Caenorhabdi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/2/172 |
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| author | Orçun Haçariz Charles Viau Xue Gu Jianguo Xia |
| author_facet | Orçun Haçariz Charles Viau Xue Gu Jianguo Xia |
| author_sort | Orçun Haçariz |
| collection | DOAJ |
| description | The roles of the healthy microbiome on the host and the relationships between members of the microbiome remain to be fully characterized. Due to the complexity of the interactions between the mammalian microbiome and its host, the use of model organisms such as the nematode worm <i>Caenorhabditis elegans</i> is a promising strategy to study host-microbiome interactions in vivo, as well as bacterial crosstalk within the host. Previously it was found that native bacterial isolates of the worm, <i>Chryseobacterium</i> sp. CHNTR56 MYb120 and <i>Comamonas</i> sp. 12022 MYb131, possess genomic diversity in the biosynthesis of the active form of vitamin B6, pyridoxal 5′-phosphate (PLP), and contribute to host fitness and lifespan extension. However, the relative contribution of PLP from each isolate, as well as the existence of interbacterial relationships within the worm gut remain to be characterized. In the present work, we investigated the presence and measured the abundance of PLP in the isolates and in the worms grown with the isolates using ultraperformance liquid chromatography tandem-mass spectrometry (UPLC-MS/MS). Our analyses confirmed the presence of PLP in vitro and in vivo. The elevated abundance of PLP in the isolates (which reached statistically significant levels when the two isolates were combined), and within worms grown with the combination of bacterial isolates, compared to control, indicated synergism between the isolates in the production of PLP. Isotope labeling revealed that <i>Comamonas</i> sp. 12022 MYb131 was the main provider of PLP in worms grown with the combination of bacterial isolates. The dominance of this isolate inside the worm was further confirmed by a colonization assay. An untargeted metabolomics analysis of the bacteria showed that the pathways related to cell growth, protein synthesis and lipid synthesis/energy production were regulated in the combination group in comparison with <i>Comamonas</i> sp. 12022 MYb131 alone. Furthermore, glutamine, involved in the <i>de novo</i> synthesis of purine and pyrimidines, was specifically abundant in this group, indicating the potential role of this metabolite in initiating and sustaining bacterial growth. This bacterial crosstalk is suggested to promote the growth of <i>Comamonas</i> sp. 12022 MYb131 in vivo, and synthesis of bacterial metabolites such as PLP in the worm gut. |
| first_indexed | 2024-03-09T21:27:17Z |
| format | Article |
| id | doaj.art-2a30d9d82d8846b091796112adb87d28 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T21:27:17Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-2a30d9d82d8846b091796112adb87d282023-11-23T21:05:31ZengMDPI AGMetabolites2218-19892022-02-0112217210.3390/metabo12020172Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-PhosphateOrçun Haçariz0Charles Viau1Xue Gu2Jianguo Xia3Institute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaInstitute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaInstitute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaInstitute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaThe roles of the healthy microbiome on the host and the relationships between members of the microbiome remain to be fully characterized. Due to the complexity of the interactions between the mammalian microbiome and its host, the use of model organisms such as the nematode worm <i>Caenorhabditis elegans</i> is a promising strategy to study host-microbiome interactions in vivo, as well as bacterial crosstalk within the host. Previously it was found that native bacterial isolates of the worm, <i>Chryseobacterium</i> sp. CHNTR56 MYb120 and <i>Comamonas</i> sp. 12022 MYb131, possess genomic diversity in the biosynthesis of the active form of vitamin B6, pyridoxal 5′-phosphate (PLP), and contribute to host fitness and lifespan extension. However, the relative contribution of PLP from each isolate, as well as the existence of interbacterial relationships within the worm gut remain to be characterized. In the present work, we investigated the presence and measured the abundance of PLP in the isolates and in the worms grown with the isolates using ultraperformance liquid chromatography tandem-mass spectrometry (UPLC-MS/MS). Our analyses confirmed the presence of PLP in vitro and in vivo. The elevated abundance of PLP in the isolates (which reached statistically significant levels when the two isolates were combined), and within worms grown with the combination of bacterial isolates, compared to control, indicated synergism between the isolates in the production of PLP. Isotope labeling revealed that <i>Comamonas</i> sp. 12022 MYb131 was the main provider of PLP in worms grown with the combination of bacterial isolates. The dominance of this isolate inside the worm was further confirmed by a colonization assay. An untargeted metabolomics analysis of the bacteria showed that the pathways related to cell growth, protein synthesis and lipid synthesis/energy production were regulated in the combination group in comparison with <i>Comamonas</i> sp. 12022 MYb131 alone. Furthermore, glutamine, involved in the <i>de novo</i> synthesis of purine and pyrimidines, was specifically abundant in this group, indicating the potential role of this metabolite in initiating and sustaining bacterial growth. This bacterial crosstalk is suggested to promote the growth of <i>Comamonas</i> sp. 12022 MYb131 in vivo, and synthesis of bacterial metabolites such as PLP in the worm gut.https://www.mdpi.com/2218-1989/12/2/172<i>C. elegans</i>microbiomevitamin B6pyridoxal 5′-phosphateglutamineLC-MS/MS |
| spellingShingle | Orçun Haçariz Charles Viau Xue Gu Jianguo Xia Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate Metabolites <i>C. elegans</i> microbiome vitamin B6 pyridoxal 5′-phosphate glutamine LC-MS/MS |
| title | Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate |
| title_full | Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate |
| title_fullStr | Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate |
| title_full_unstemmed | Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate |
| title_short | Native Microbiome Members of <i>C. elegans</i> Act Synergistically in Biosynthesis of Pyridoxal 5′-Phosphate |
| title_sort | native microbiome members of i c elegans i act synergistically in biosynthesis of pyridoxal 5 phosphate |
| topic | <i>C. elegans</i> microbiome vitamin B6 pyridoxal 5′-phosphate glutamine LC-MS/MS |
| url | https://www.mdpi.com/2218-1989/12/2/172 |
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