Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i>
To explore bioactive polysaccharides from the byproducts of squid processing, a heteropolysaccharide, named SV2-1, was isolated from the viscera of squid <i>Ommastrephes bartrami</i> by autolysis, anion-exchange and gel-permeation chromatography and measured for its neuroprotective activ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/3/188 |
| _version_ | 1811278532100227072 |
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| author | Peng Ye Peipei Li Wenge Yang Yue Zhao Yuqin Zhao Kunlai Sun Bin Wang Yin Chen |
| author_facet | Peng Ye Peipei Li Wenge Yang Yue Zhao Yuqin Zhao Kunlai Sun Bin Wang Yin Chen |
| author_sort | Peng Ye |
| collection | DOAJ |
| description | To explore bioactive polysaccharides from the byproducts of squid processing, a heteropolysaccharide, named SV2-1, was isolated from the viscera of squid <i>Ommastrephes bartrami</i> by autolysis, anion-exchange and gel-permeation chromatography and measured for its neuroprotective activity. It was a homogeneous polysaccharide with a molecular weight of 2.3 kDa by HPSEC analysis. SV2-1 contained glucuronic acid, galactosamine and fucose in the ratio of 1.0:1.1:1.2. Its structural characteristics were elucidated by methylation analysis, gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The backbone of SV2-1 was composed of alternant →4)-α-<span style="font-variant: small-caps;">l</span>-Fuc<i>p</i>-(1→ and →3)-β-<span style="font-variant: small-caps;">d</span>-GlcUA-(1→ Most of →4)-α-<span style="font-variant: small-caps;">l</span>-Fuc<i>p</i>-(1→ (90%) was substituted by single α-<span style="font-variant: small-caps;">d</span>-GlcNAc as the branches. SV2-1 can protect against the death of PC12 induced by 6-OHDA, and effectively improves cell viability and reduces extracellular LDH release in PC12 cells after injury. Moreover, SV2-1 significantly increases SOD activity but decreases MDA levels. |
| first_indexed | 2024-04-13T00:37:31Z |
| format | Article |
| id | doaj.art-2a324464858c4816986d54eb1e7a8738 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T00:37:31Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-2a324464858c4816986d54eb1e7a87382022-12-22T03:10:17ZengMDPI AGMarine Drugs1660-33972019-03-0117318810.3390/md17030188md17030188Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i>Peng Ye0Peipei Li1Wenge Yang2Yue Zhao3Yuqin Zhao4Kunlai Sun5Bin Wang6Yin Chen7College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaMarine School, Ningbo University, Ningbo 315000, ChinaMarine School, Ningbo University, Ningbo 315000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaTo explore bioactive polysaccharides from the byproducts of squid processing, a heteropolysaccharide, named SV2-1, was isolated from the viscera of squid <i>Ommastrephes bartrami</i> by autolysis, anion-exchange and gel-permeation chromatography and measured for its neuroprotective activity. It was a homogeneous polysaccharide with a molecular weight of 2.3 kDa by HPSEC analysis. SV2-1 contained glucuronic acid, galactosamine and fucose in the ratio of 1.0:1.1:1.2. Its structural characteristics were elucidated by methylation analysis, gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The backbone of SV2-1 was composed of alternant →4)-α-<span style="font-variant: small-caps;">l</span>-Fuc<i>p</i>-(1→ and →3)-β-<span style="font-variant: small-caps;">d</span>-GlcUA-(1→ Most of →4)-α-<span style="font-variant: small-caps;">l</span>-Fuc<i>p</i>-(1→ (90%) was substituted by single α-<span style="font-variant: small-caps;">d</span>-GlcNAc as the branches. SV2-1 can protect against the death of PC12 induced by 6-OHDA, and effectively improves cell viability and reduces extracellular LDH release in PC12 cells after injury. Moreover, SV2-1 significantly increases SOD activity but decreases MDA levels.https://www.mdpi.com/1660-3397/17/3/188polysaccharidesquid autolysatestructureneuroprotective |
| spellingShingle | Peng Ye Peipei Li Wenge Yang Yue Zhao Yuqin Zhao Kunlai Sun Bin Wang Yin Chen Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> Marine Drugs polysaccharide squid autolysate structure neuroprotective |
| title | Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> |
| title_full | Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> |
| title_fullStr | Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> |
| title_full_unstemmed | Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> |
| title_short | Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of Squid <i>Ommastrephes bartrami</i> |
| title_sort | structure and neuroprotective effect of polysaccharide from viscera autolysates of squid i ommastrephes bartrami i |
| topic | polysaccharide squid autolysate structure neuroprotective |
| url | https://www.mdpi.com/1660-3397/17/3/188 |
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