Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis
This study attempted to clarify RpL10a function in testis using mouse testis as a model. The histological method of mouse testis was used to determine the percentages of cell types in the spermatogenesis after being treated with a recombinant RpL10a protein (rRpL10a). The percentages of spermatid...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2019-10-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/41-5/1.pdf |
| _version_ | 1828542093271760896 |
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| author | Kunwadee Palasin Walaiporn Makkapan Monwadee Wonglapsuwan Wilaiwan Chotigeat |
| author_facet | Kunwadee Palasin Walaiporn Makkapan Monwadee Wonglapsuwan Wilaiwan Chotigeat |
| author_sort | Kunwadee Palasin |
| collection | DOAJ |
| description | This study attempted to clarify RpL10a function in testis using mouse testis as a model. The histological method of
mouse testis was used to determine the percentages of cell types in the spermatogenesis after being treated with a recombinant
RpL10a protein (rRpL10a). The percentages of spermatid cells in the testis treated with 0.5, 1.0, 1.5, and 2.0 µM of rRpL10a
protein were 40.78%, 49.65%, 45.03%, and 45.33%, respectively, while the untreated group was 21.72%. The spermatid cells of
the experiment tissue were confirmed by WGA lectin histochemistry. Prm2 gene expression was moderately increased, whereas
the expression of Rhau gene decreased in rRpL10a treated testes compared to the untreated testis. Furthermore, increased cell
proliferation in the testes treated with 1.0 µM rRpL10a was revealed by an intense fluorescent signal of EdU (5-ethynyl-2′-
deoxyuridine) staining. Altogether, the results indicated that the rRpL10a protein induced spermatogenesis in mice and may be
applicable for in vitro spermatogenesis induction in the future. |
| first_indexed | 2024-12-12T01:50:11Z |
| format | Article |
| id | doaj.art-2a32520a50ee49e2a2918f438f14fd59 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-12T01:50:11Z |
| publishDate | 2019-10-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-2a32520a50ee49e2a2918f438f14fd592022-12-22T00:42:30ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952019-10-0141595996510.14456/sjst-psu.2019.121Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesisKunwadee Palasin0Walaiporn Makkapan1Monwadee Wonglapsuwan2Wilaiwan Chotigeat3Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandBiotechnology Program, Department of General Science and Liberal Arts, King Mongkut's Institute of Technology Ladkrabang, Prince of Chumphon Campus, Pathio, Chumphon, 86160 ThailandDepartment of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandDepartment of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandThis study attempted to clarify RpL10a function in testis using mouse testis as a model. The histological method of mouse testis was used to determine the percentages of cell types in the spermatogenesis after being treated with a recombinant RpL10a protein (rRpL10a). The percentages of spermatid cells in the testis treated with 0.5, 1.0, 1.5, and 2.0 µM of rRpL10a protein were 40.78%, 49.65%, 45.03%, and 45.33%, respectively, while the untreated group was 21.72%. The spermatid cells of the experiment tissue were confirmed by WGA lectin histochemistry. Prm2 gene expression was moderately increased, whereas the expression of Rhau gene decreased in rRpL10a treated testes compared to the untreated testis. Furthermore, increased cell proliferation in the testes treated with 1.0 µM rRpL10a was revealed by an intense fluorescent signal of EdU (5-ethynyl-2′- deoxyuridine) staining. Altogether, the results indicated that the rRpL10a protein induced spermatogenesis in mice and may be applicable for in vitro spermatogenesis induction in the future.https://rdo.psu.ac.th/sjstweb/journal/41-5/1.pdfrrpl10aspermatogenesistestismice |
| spellingShingle | Kunwadee Palasin Walaiporn Makkapan Monwadee Wonglapsuwan Wilaiwan Chotigeat Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis Songklanakarin Journal of Science and Technology (SJST) rrpl10a spermatogenesis testis mice |
| title | Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis |
| title_full | Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis |
| title_fullStr | Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis |
| title_full_unstemmed | Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis |
| title_short | Effect of a recombinant ribosomal protein L10a (rRpL10a) on mouse spermatogenesis |
| title_sort | effect of a recombinant ribosomal protein l10a rrpl10a on mouse spermatogenesis |
| topic | rrpl10a spermatogenesis testis mice |
| url | https://rdo.psu.ac.th/sjstweb/journal/41-5/1.pdf |
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