Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems
Bunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic pain and dysmenorrhoea. The aim of the current study was to determine antinociceptive mechanisms of B...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-02-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201502-0001_antinociceptive-mechanisms-of-bunium-persicum-essential-oil-in-the-mouse-writhing-test-role-of-opioidergic-and.php |
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| author | M. Zendehdel Z. Torabi S. Hassanpour |
| author_facet | M. Zendehdel Z. Torabi S. Hassanpour |
| author_sort | M. Zendehdel |
| collection | DOAJ |
| description | Bunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic pain and dysmenorrhoea. The aim of the current study was to determine antinociceptive mechanisms of B. persicum essential oil using an acetic acid-induced writhing test as a model of visceral pain and to determine the possible involvement of opioidergic, serotoninergic and histaminergic systems on antinociceptive mechanisms of B. persicum in male mice. In experiment 1, B. persicum was intraperitoneally (i.p.) injected (0.001, 0.01, 0.05, 0.1, 0.5 and 1%; 10 ml/kg) in Tween-80 (0.5%) and a writhing test served as a model of visceral pain. In experiments 2-5, opioidergic receptor antagonist (naloxone, 2 mg/kg), serotonergic receptor antagonist (cyproheptadine, 4 mg/kg), histamine H1-receptor antagonist (chlorpheniramine, 20 mg/kg) and histamine H2-receptor antagonist (cimetidine, 12.5mg/kg) injection was followed by B. persicum (0.01%; 10 ml/kg) and the writhing test response was measured for 30 min. According to the results, essential oil of B. persicum, administered i.p. (0.001, 0.01, 0.05, 0.1, 0.5, and 1%; 10 ml/kg) in Tween-80 (0.5%), elicited antinociceptive effects in a dose-dependent manner. Moreover, the antinociceptive effect of B. persicum was significantly attenuated by pre-treatment with naloxone, chlorpheniramine and cimetidine (P < 0.001). These results suggest that B. persicum-induced analgesia may be mediated via opioidergic and histamine H1 and H2 receptors. |
| first_indexed | 2024-04-10T07:59:24Z |
| format | Article |
| id | doaj.art-2a3500e7ad8e4c3aba147702f4481b87 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:24Z |
| publishDate | 2015-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Veterinární Medicína |
| spelling | doaj.art-2a3500e7ad8e4c3aba147702f4481b872023-02-23T03:50:23ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922015-02-01602637010.17221/7988-VETMEDvet-201502-0001Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systemsM. Zendehdel0Z. Torabi1S. Hassanpour2Faculty of Veterinary Medicine, University of Tehran, Tehran, IranFaculty of Basic Sciences, Islamic Azad University of Damghan, Damghan, IranFaculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, IranBunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic pain and dysmenorrhoea. The aim of the current study was to determine antinociceptive mechanisms of B. persicum essential oil using an acetic acid-induced writhing test as a model of visceral pain and to determine the possible involvement of opioidergic, serotoninergic and histaminergic systems on antinociceptive mechanisms of B. persicum in male mice. In experiment 1, B. persicum was intraperitoneally (i.p.) injected (0.001, 0.01, 0.05, 0.1, 0.5 and 1%; 10 ml/kg) in Tween-80 (0.5%) and a writhing test served as a model of visceral pain. In experiments 2-5, opioidergic receptor antagonist (naloxone, 2 mg/kg), serotonergic receptor antagonist (cyproheptadine, 4 mg/kg), histamine H1-receptor antagonist (chlorpheniramine, 20 mg/kg) and histamine H2-receptor antagonist (cimetidine, 12.5mg/kg) injection was followed by B. persicum (0.01%; 10 ml/kg) and the writhing test response was measured for 30 min. According to the results, essential oil of B. persicum, administered i.p. (0.001, 0.01, 0.05, 0.1, 0.5, and 1%; 10 ml/kg) in Tween-80 (0.5%), elicited antinociceptive effects in a dose-dependent manner. Moreover, the antinociceptive effect of B. persicum was significantly attenuated by pre-treatment with naloxone, chlorpheniramine and cimetidine (P < 0.001). These results suggest that B. persicum-induced analgesia may be mediated via opioidergic and histamine H1 and H2 receptors.https://vetmed.agriculturejournals.cz/artkey/vet-201502-0001_antinociceptive-mechanisms-of-bunium-persicum-essential-oil-in-the-mouse-writhing-test-role-of-opioidergic-and.phpbunium persicumantinociceptionwrithing testmouse |
| spellingShingle | M. Zendehdel Z. Torabi S. Hassanpour Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems Veterinární Medicína bunium persicum antinociception writhing test mouse |
| title | Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems |
| title_full | Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems |
| title_fullStr | Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems |
| title_full_unstemmed | Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems |
| title_short | Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems |
| title_sort | antinociceptive mechanisms of bunium persicum essential oil in the mouse writhing test role of opioidergic and histaminergic systems |
| topic | bunium persicum antinociception writhing test mouse |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201502-0001_antinociceptive-mechanisms-of-bunium-persicum-essential-oil-in-the-mouse-writhing-test-role-of-opioidergic-and.php |
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