Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients
Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. Serum microRNA (miRNA) has been reported as a biomarker for various conditions of allograft injuries. The upregulation of miR-21 is the best-known miRNA change in graft tissue, urine and plasma. Howev...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12253 |
| _version_ | 1797472745185869824 |
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| author | Yu-Jen Chen Chia-Tien Hsu Shang-Feng Tsai Cheng-Hsu Chen |
| author_facet | Yu-Jen Chen Chia-Tien Hsu Shang-Feng Tsai Cheng-Hsu Chen |
| author_sort | Yu-Jen Chen |
| collection | DOAJ |
| description | Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. Serum microRNA (miRNA) has been reported as a biomarker for various conditions of allograft injuries. The upregulation of miR-21 is the best-known miRNA change in graft tissue, urine and plasma. However, the correlation of plasma miR-21 with the severity of CAD remains unclear. In our study, 40 kidney transplantation recipients with late graft survival for more than 10 years were enrolled. The CAD group (<i>n</i> = 20) had either an eGFR between 15 to 60 mL/min or a biopsy-proved chronic allograft nephropathy or rejection. The control group (<i>n</i> = 20) had an eGFR ≥ 60 mL/min without proteinuria and hematuria for a consecutive 3 months before the study. We performed RNA sequencing to profile the miRNAs expression. There were six differentially expressed miRNAs in the CAD group. Among them, miR-21-5p and miR-101-3p were decreased, and miR-20a-5p was increased. We found that miR-21-5p, miR-20a-5p and miR-101-3p all participated in the TGF-beta pathway. We demonstrated that decreased miR-21-5p and miR-101-3p, and increased miR-20a-5p were the novel CAD-associated miRNAs in the TGF-beta pathway. These findings may pave the way for developing early prediction miRNAs biomarkers for CAD, and possibly developing therapeutic tools in the field of kidney transplantation. |
| first_indexed | 2024-03-09T20:06:32Z |
| format | Article |
| id | doaj.art-2a351c9b94da48269b2b21cc9cbaabb4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:06:32Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2a351c9b94da48269b2b21cc9cbaabb42023-11-24T00:30:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201225310.3390/ijms232012253Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant RecipientsYu-Jen Chen0Chia-Tien Hsu1Shang-Feng Tsai2Cheng-Hsu Chen3Division of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407219, TaiwanDivision of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407219, TaiwanDivision of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407219, TaiwanDivision of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407219, TaiwanChronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. Serum microRNA (miRNA) has been reported as a biomarker for various conditions of allograft injuries. The upregulation of miR-21 is the best-known miRNA change in graft tissue, urine and plasma. However, the correlation of plasma miR-21 with the severity of CAD remains unclear. In our study, 40 kidney transplantation recipients with late graft survival for more than 10 years were enrolled. The CAD group (<i>n</i> = 20) had either an eGFR between 15 to 60 mL/min or a biopsy-proved chronic allograft nephropathy or rejection. The control group (<i>n</i> = 20) had an eGFR ≥ 60 mL/min without proteinuria and hematuria for a consecutive 3 months before the study. We performed RNA sequencing to profile the miRNAs expression. There were six differentially expressed miRNAs in the CAD group. Among them, miR-21-5p and miR-101-3p were decreased, and miR-20a-5p was increased. We found that miR-21-5p, miR-20a-5p and miR-101-3p all participated in the TGF-beta pathway. We demonstrated that decreased miR-21-5p and miR-101-3p, and increased miR-20a-5p were the novel CAD-associated miRNAs in the TGF-beta pathway. These findings may pave the way for developing early prediction miRNAs biomarkers for CAD, and possibly developing therapeutic tools in the field of kidney transplantation.https://www.mdpi.com/1422-0067/23/20/12253chronic allograft dysfunctionmicroRNAbiomarker |
| spellingShingle | Yu-Jen Chen Chia-Tien Hsu Shang-Feng Tsai Cheng-Hsu Chen Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients International Journal of Molecular Sciences chronic allograft dysfunction microRNA biomarker |
| title | Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients |
| title_full | Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients |
| title_fullStr | Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients |
| title_full_unstemmed | Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients |
| title_short | Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients |
| title_sort | association between circulating micrornas mir 21 5p mir 20a 5p mir 29b 3p mir 126 3p and mir 101 3p and chronic allograft dysfunction in renal transplant recipients |
| topic | chronic allograft dysfunction microRNA biomarker |
| url | https://www.mdpi.com/1422-0067/23/20/12253 |
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