Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts
Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/9/1868 |
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| author | Anna Löfdahl Andreas Jern Samuel Flyman Monica Kåredal Hanna L Karlsson Anna-Karin Larsson-Callerfelt |
| author_facet | Anna Löfdahl Andreas Jern Samuel Flyman Monica Kåredal Hanna L Karlsson Anna-Karin Larsson-Callerfelt |
| author_sort | Anna Löfdahl |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 μg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced metabolic activities at 10 μg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly increased procollagen I synthesis and release of IL-8, prostaglandin E<sub>2</sub>, RANTES and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with altered procollagen and proinflammatory cytokine secretion in fibroblasts over time. Hence, careful characterizations of AgNPs are of importance, and future studies should include timepoints beyond 24 h. |
| first_indexed | 2024-03-10T16:14:17Z |
| format | Article |
| id | doaj.art-2a3646996c734a62983017fb2aa97627 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T16:14:17Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-2a3646996c734a62983017fb2aa976272023-11-20T14:11:20ZengMDPI AGNanomaterials2079-49912020-09-01109186810.3390/nano10091868Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung FibroblastsAnna Löfdahl0Andreas Jern1Samuel Flyman2Monica Kåredal3Hanna L Karlsson4Anna-Karin Larsson-Callerfelt5Unit of Lung Biology, Department of Experimental Medical Sciences, Lund University, SE-221 00 Lund, SwedenUnit of Lung Biology, Department of Experimental Medical Sciences, Lund University, SE-221 00 Lund, SwedenUnit of Lung Biology, Department of Experimental Medical Sciences, Lund University, SE-221 00 Lund, SwedenDivision of Occupational and Environmental Medicine, Lund University, SE-221 00 Lund, SwedenUnit of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-171 76 Stockholm, SwedenUnit of Lung Biology, Department of Experimental Medical Sciences, Lund University, SE-221 00 Lund, SwedenSilver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 μg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced metabolic activities at 10 μg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly increased procollagen I synthesis and release of IL-8, prostaglandin E<sub>2</sub>, RANTES and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with altered procollagen and proinflammatory cytokine secretion in fibroblasts over time. Hence, careful characterizations of AgNPs are of importance, and future studies should include timepoints beyond 24 h.https://www.mdpi.com/2079-4991/10/9/1868silver nanoparticleshuman lung fibroblastsprecision-cut lung slicestoxicityextracellular matrixprocollagen |
| spellingShingle | Anna Löfdahl Andreas Jern Samuel Flyman Monica Kåredal Hanna L Karlsson Anna-Karin Larsson-Callerfelt Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts Nanomaterials silver nanoparticles human lung fibroblasts precision-cut lung slices toxicity extracellular matrix procollagen |
| title | Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts |
| title_full | Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts |
| title_fullStr | Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts |
| title_full_unstemmed | Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts |
| title_short | Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts |
| title_sort | silver nanoparticles alter cell viability ex vivo and in vitro and induce proinflammatory effects in human lung fibroblasts |
| topic | silver nanoparticles human lung fibroblasts precision-cut lung slices toxicity extracellular matrix procollagen |
| url | https://www.mdpi.com/2079-4991/10/9/1868 |
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