Heterologous microsatellite primer pairs informative for the whole genus Arachis
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the sel...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016 |
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| author | Andrea Akemi Hoshino Juliana Pereira Bravo Carla M.L.C.D. Angelici Andréa V. Gobbi Barbosa Catalina Romero Lopes Marcos A. Gimenes |
| author_facet | Andrea Akemi Hoshino Juliana Pereira Bravo Carla M.L.C.D. Angelici Andréa V. Gobbi Barbosa Catalina Romero Lopes Marcos A. Gimenes |
| author_sort | Andrea Akemi Hoshino |
| collection | DOAJ |
| description | The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions. |
| first_indexed | 2024-12-21T03:05:45Z |
| format | Article |
| id | doaj.art-2a3656a5984544b09059e6a7a72c3e2e |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T03:05:45Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-2a3656a5984544b09059e6a7a72c3e2e2022-12-21T19:18:05ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129466567510.1590/S1415-47572006000400016Heterologous microsatellite primer pairs informative for the whole genus ArachisAndrea Akemi HoshinoJuliana Pereira BravoCarla M.L.C.D. AngeliciAndréa V. Gobbi BarbosaCatalina Romero LopesMarcos A. GimenesThe genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016peanutgenetic relationshipmolecular markerswild relatives |
| spellingShingle | Andrea Akemi Hoshino Juliana Pereira Bravo Carla M.L.C.D. Angelici Andréa V. Gobbi Barbosa Catalina Romero Lopes Marcos A. Gimenes Heterologous microsatellite primer pairs informative for the whole genus Arachis Genetics and Molecular Biology peanut genetic relationship molecular markers wild relatives |
| title | Heterologous microsatellite primer pairs informative for the whole genus Arachis |
| title_full | Heterologous microsatellite primer pairs informative for the whole genus Arachis |
| title_fullStr | Heterologous microsatellite primer pairs informative for the whole genus Arachis |
| title_full_unstemmed | Heterologous microsatellite primer pairs informative for the whole genus Arachis |
| title_short | Heterologous microsatellite primer pairs informative for the whole genus Arachis |
| title_sort | heterologous microsatellite primer pairs informative for the whole genus arachis |
| topic | peanut genetic relationship molecular markers wild relatives |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016 |
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