Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49
Thiazolidinedione 49 (TD<sub>49</sub>), a newly synthesized algicide, shows strong toxicity at low concentrations of 0.1−2.0 μM. However, its potential effects on non-target species at the transcript level were not investigated. Differentially expressed genes (DEGs) in...
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MDPI AG
2019-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/8/310 |
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| author | Cheng Chi Sib Sankar Giri Jin Woo Jun Hyoun Joong Kim Saekil Yun Sang Wha Kim Jeong Woo Kang Se Chang Park |
| author_facet | Cheng Chi Sib Sankar Giri Jin Woo Jun Hyoun Joong Kim Saekil Yun Sang Wha Kim Jeong Woo Kang Se Chang Park |
| author_sort | Cheng Chi |
| collection | DOAJ |
| description | Thiazolidinedione 49 (TD<sub>49</sub>), a newly synthesized algicide, shows strong toxicity at low concentrations of 0.1−2.0 μM. However, its potential effects on non-target species at the transcript level were not investigated. Differentially expressed genes (DEGs) in the gills of the bay scallop, <i>Argopecten irradians</i>, were accessed after treatment with 0.68 μM TD<sub>49</sub> for up to 48 h. Following exposure, it was observed that 5214 genes were upregulated and 3497 were downregulated. Functional enrichment analysis revealed that the apoptosis pathway was activated. The extrinsic apoptosis pathway was activated and the survival factors related pathway was suppressed. Furthermore, gene expressions related to ATP-binding cassette, nuclear factor erythroid 2-related factor, B cell lymphoma-2 family protein, glutathione reductase, glutathione peroxidase, catalase, NADPH2:quinone reductase, and superoxide dismutase were decreased. Conversely, gene expressions related to FAS-associated death domain protein, glutathione <i>S</i>-transferase, caspase 6, 8, cytochrome P450 1A1, and 2C8 were increased. These results comprehensively demonstrated the toxicity of the novel algicide TD<sub>49</sub>, and should draw the attention of researchers to the importance of analyzing the potential impact of chemical compounds as algicides to control the proliferation of harmful algae, due to the secondary pollution caused by their application. |
| first_indexed | 2024-12-11T03:04:41Z |
| format | Article |
| id | doaj.art-2a36bc696ca44b23ab31e990861aa74e |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T03:04:41Z |
| publishDate | 2019-07-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2a36bc696ca44b23ab31e990861aa74e2022-12-22T01:22:58ZengMDPI AGBiomolecules2218-273X2019-07-019831010.3390/biom9080310biom9080310Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49Cheng Chi0Sib Sankar Giri1Jin Woo Jun2Hyoun Joong Kim3Saekil Yun4Sang Wha Kim5Jeong Woo Kang6Se Chang Park7Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Weigang Road 1, Nanjing 210095, ChinaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaDepartment of Aquaculture, Korea National College of Agriculture and Fisheries, Jeonju 54874, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaThiazolidinedione 49 (TD<sub>49</sub>), a newly synthesized algicide, shows strong toxicity at low concentrations of 0.1−2.0 μM. However, its potential effects on non-target species at the transcript level were not investigated. Differentially expressed genes (DEGs) in the gills of the bay scallop, <i>Argopecten irradians</i>, were accessed after treatment with 0.68 μM TD<sub>49</sub> for up to 48 h. Following exposure, it was observed that 5214 genes were upregulated and 3497 were downregulated. Functional enrichment analysis revealed that the apoptosis pathway was activated. The extrinsic apoptosis pathway was activated and the survival factors related pathway was suppressed. Furthermore, gene expressions related to ATP-binding cassette, nuclear factor erythroid 2-related factor, B cell lymphoma-2 family protein, glutathione reductase, glutathione peroxidase, catalase, NADPH2:quinone reductase, and superoxide dismutase were decreased. Conversely, gene expressions related to FAS-associated death domain protein, glutathione <i>S</i>-transferase, caspase 6, 8, cytochrome P450 1A1, and 2C8 were increased. These results comprehensively demonstrated the toxicity of the novel algicide TD<sub>49</sub>, and should draw the attention of researchers to the importance of analyzing the potential impact of chemical compounds as algicides to control the proliferation of harmful algae, due to the secondary pollution caused by their application.https://www.mdpi.com/2218-273X/9/8/310algicideThiazolidinedione 49bay scalloptranscriptomic analysisapoptosisdetoxificationimmunity |
| spellingShingle | Cheng Chi Sib Sankar Giri Jin Woo Jun Hyoun Joong Kim Saekil Yun Sang Wha Kim Jeong Woo Kang Se Chang Park Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 Biomolecules algicide Thiazolidinedione 49 bay scallop transcriptomic analysis apoptosis detoxification immunity |
| title | Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 |
| title_full | Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 |
| title_fullStr | Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 |
| title_full_unstemmed | Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 |
| title_short | Detoxification, Apoptosis, and Immune Transcriptomic Responses of the Gill Tissue of Bay Scallop Following Exposure to the Algicide Thiazolidinedione 49 |
| title_sort | detoxification apoptosis and immune transcriptomic responses of the gill tissue of bay scallop following exposure to the algicide thiazolidinedione 49 |
| topic | algicide Thiazolidinedione 49 bay scallop transcriptomic analysis apoptosis detoxification immunity |
| url | https://www.mdpi.com/2218-273X/9/8/310 |
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