17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>)
<p>Abstract</p> <p>This study was performed to investigate stress effects on the synthesis of egg yolk precursor, vitellogenin (Vtg) in Arctic char (<it>Salvelinus alpinus</it>). In particular the effect of cortisol (F) was determined since this stress hormone has been...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-09-01
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| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/2/1/62 |
| _version_ | 1818750391211261952 |
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| author | Modig Carina Berg Hakan Olsson Per-Erik |
| author_facet | Modig Carina Berg Hakan Olsson Per-Erik |
| author_sort | Modig Carina |
| collection | DOAJ |
| description | <p>Abstract</p> <p>This study was performed to investigate stress effects on the synthesis of egg yolk precursor, vitellogenin (Vtg) in Arctic char (<it>Salvelinus alpinus</it>). In particular the effect of cortisol (F) was determined since this stress hormone has been suggested to interfere with vitellogenesis and is upregulated during sexual maturation in teleosts. Arctic char Vtg was purified and polyclonal antibodies were produced in order to develop tools to study regulation of vitellogenesis. The Vtg antibodies were used to develop an enzyme-linked immunosorbent assay. The corresponding Vtg cDNA was cloned from a hepatic cDNA library in order to obtain DNA probes to measure Vtg mRNA expression. Analysis of plasma from juvenile Arctic char, of both sexes, exposed to different steroids showed that production of Vtg was induced in a dose dependent fashion by 17β-estradiol (E2), estrone and estriol. Apart from estrogens a high dose of F also upregulated Vtg. In addition, F, progesterone (P) and tamoxifen were tested to determine these compounds ability to modulate E2 induced Vtg synthesis at both the mRNA and protein level. Tamoxifen was found to inhibit E2 induced Vtg mRNA and protein upregulation. P did not alter the Vtg induction while F reduced the Vtg protein levels without affecting the Vtg mRNA levels. Furthermore the inhibition of Vtg protein was found to be dose dependent. Thus, the inhibitory effect of F on Vtg appears to be mediated at the post-transcriptional level.</p> |
| first_indexed | 2024-12-18T04:18:56Z |
| format | Article |
| id | doaj.art-2a3840239bf3454dad0783eab4fb2113 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-18T04:18:56Z |
| publishDate | 2004-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-2a3840239bf3454dad0783eab4fb21132022-12-21T21:21:17ZengBMCReproductive Biology and Endocrinology1477-78272004-09-01216210.1186/1477-7827-2-6217beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>)Modig CarinaBerg HakanOlsson Per-Erik<p>Abstract</p> <p>This study was performed to investigate stress effects on the synthesis of egg yolk precursor, vitellogenin (Vtg) in Arctic char (<it>Salvelinus alpinus</it>). In particular the effect of cortisol (F) was determined since this stress hormone has been suggested to interfere with vitellogenesis and is upregulated during sexual maturation in teleosts. Arctic char Vtg was purified and polyclonal antibodies were produced in order to develop tools to study regulation of vitellogenesis. The Vtg antibodies were used to develop an enzyme-linked immunosorbent assay. The corresponding Vtg cDNA was cloned from a hepatic cDNA library in order to obtain DNA probes to measure Vtg mRNA expression. Analysis of plasma from juvenile Arctic char, of both sexes, exposed to different steroids showed that production of Vtg was induced in a dose dependent fashion by 17β-estradiol (E2), estrone and estriol. Apart from estrogens a high dose of F also upregulated Vtg. In addition, F, progesterone (P) and tamoxifen were tested to determine these compounds ability to modulate E2 induced Vtg synthesis at both the mRNA and protein level. Tamoxifen was found to inhibit E2 induced Vtg mRNA and protein upregulation. P did not alter the Vtg induction while F reduced the Vtg protein levels without affecting the Vtg mRNA levels. Furthermore the inhibition of Vtg protein was found to be dose dependent. Thus, the inhibitory effect of F on Vtg appears to be mediated at the post-transcriptional level.</p>http://www.rbej.com/content/2/1/62 |
| spellingShingle | Modig Carina Berg Hakan Olsson Per-Erik 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) Reproductive Biology and Endocrinology |
| title | 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) |
| title_full | 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) |
| title_fullStr | 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) |
| title_full_unstemmed | 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) |
| title_short | 17beta-estradiol induced vitellogenesis is inhibited by cortisol at the post-transcriptional level in Arctic char (<it>Salvelinus alpinus</it>) |
| title_sort | 17beta estradiol induced vitellogenesis is inhibited by cortisol at the post transcriptional level in arctic char it salvelinus alpinus it |
| url | http://www.rbej.com/content/2/1/62 |
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