Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma
Objective Glioblastoma (GBM), a type of malignant glioma, is the most aggressive type of brain tumor and is associated with high mortality. Hexose-6-phosphate dehydrogenase (H6PD) has been detected in multiple tumors and is involved in tumor initiation and progression. However, the specific role and...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2076-3425/12/8/1012 |
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| author | Yi-Bin Zhang Shu-Fa Zheng Lin-Jie Ma Peng Lin Huang-Cheng Shang-Guan Yuan-Xiang Lin De-Zhi Kang Pei-Sen Yao |
| author_facet | Yi-Bin Zhang Shu-Fa Zheng Lin-Jie Ma Peng Lin Huang-Cheng Shang-Guan Yuan-Xiang Lin De-Zhi Kang Pei-Sen Yao |
| author_sort | Yi-Bin Zhang |
| collection | DOAJ |
| description | Objective Glioblastoma (GBM), a type of malignant glioma, is the most aggressive type of brain tumor and is associated with high mortality. Hexose-6-phosphate dehydrogenase (H6PD) has been detected in multiple tumors and is involved in tumor initiation and progression. However, the specific role and mechanism of H6PD in GBM remain unclear. Methods We performed pan-cancer analysis of expression and prognosis of H6PD in GBM using the Genotype-Tissue Expression Project (GTEx) and The Cancer Genome Atlas (TCGA). Subsequently, noncoding RNAs regulating H6PD expression were obtained by comprehensive analysis, including gene expression, prognosis, correlation, and immune infiltration. Finally, tumor immune infiltrates related to H6PD and survival were performed. Results Higher expression of H6PD was statistically significantly associated with an unfavorable outcome in GBM. Downregulation of hsa-miR-124-3p and hsa-miR-516b-5p in GBM was detected from GSE90603. Subsequently, OSMR-AS1 was observed in the regulation of H6PD via hsa-miR-516b-5p. Moreover, higher H6PD expression significantly correlated with immune infiltration of dendritic cells, immune checkpoint expression, and biomarkers of dendritic cells. Conclusions The OSMR-AS1/ miR-516b-5p axis was identified as the highest-potential upstream ncRNA-related pathway of H6PD in GBM. Furthermore, the present findings demonstrated that H6PD blockading might possess antitumor roles via regulating dendritic cell infiltration and immune checkpoint expression. |
| first_indexed | 2024-03-09T09:59:07Z |
| format | Article |
| id | doaj.art-2a391ab3c691472c89d92e1a152b6930 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-09T09:59:07Z |
| publishDate | 2022-07-01 |
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| series | Brain Sciences |
| spelling | doaj.art-2a391ab3c691472c89d92e1a152b69302023-12-01T23:30:58ZengMDPI AGBrain Sciences2076-34252022-07-01128101210.3390/brainsci12081012Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of GlioblastomaYi-Bin Zhang0Shu-Fa Zheng1Lin-Jie Ma2Peng Lin3Huang-Cheng Shang-Guan4Yuan-Xiang Lin5De-Zhi Kang6Pei-Sen Yao7Department of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurology and Neurosurgery, Changji Traditional Chinese Medicine Hospital, Changji 831100, ChinaDepartment of Pain, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaDepartment of Neurosurgery, Neurosurgical Research Institute, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, ChinaObjective Glioblastoma (GBM), a type of malignant glioma, is the most aggressive type of brain tumor and is associated with high mortality. Hexose-6-phosphate dehydrogenase (H6PD) has been detected in multiple tumors and is involved in tumor initiation and progression. However, the specific role and mechanism of H6PD in GBM remain unclear. Methods We performed pan-cancer analysis of expression and prognosis of H6PD in GBM using the Genotype-Tissue Expression Project (GTEx) and The Cancer Genome Atlas (TCGA). Subsequently, noncoding RNAs regulating H6PD expression were obtained by comprehensive analysis, including gene expression, prognosis, correlation, and immune infiltration. Finally, tumor immune infiltrates related to H6PD and survival were performed. Results Higher expression of H6PD was statistically significantly associated with an unfavorable outcome in GBM. Downregulation of hsa-miR-124-3p and hsa-miR-516b-5p in GBM was detected from GSE90603. Subsequently, OSMR-AS1 was observed in the regulation of H6PD via hsa-miR-516b-5p. Moreover, higher H6PD expression significantly correlated with immune infiltration of dendritic cells, immune checkpoint expression, and biomarkers of dendritic cells. Conclusions The OSMR-AS1/ miR-516b-5p axis was identified as the highest-potential upstream ncRNA-related pathway of H6PD in GBM. Furthermore, the present findings demonstrated that H6PD blockading might possess antitumor roles via regulating dendritic cell infiltration and immune checkpoint expression.https://www.mdpi.com/2076-3425/12/8/1012H6PDceRNA networkglioblastomaimmune infiltrationoutcome |
| spellingShingle | Yi-Bin Zhang Shu-Fa Zheng Lin-Jie Ma Peng Lin Huang-Cheng Shang-Guan Yuan-Xiang Lin De-Zhi Kang Pei-Sen Yao Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma Brain Sciences H6PD ceRNA network glioblastoma immune infiltration outcome |
| title | Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma |
| title_full | Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma |
| title_fullStr | Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma |
| title_full_unstemmed | Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma |
| title_short | Elevated Hexose-6-Phosphate Dehydrogenase Regulated by OSMR-AS1/hsa-miR-516b-5p Axis Correlates with Poor Prognosis and Dendritic Cells Infiltration of Glioblastoma |
| title_sort | elevated hexose 6 phosphate dehydrogenase regulated by osmr as1 hsa mir 516b 5p axis correlates with poor prognosis and dendritic cells infiltration of glioblastoma |
| topic | H6PD ceRNA network glioblastoma immune infiltration outcome |
| url | https://www.mdpi.com/2076-3425/12/8/1012 |
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