Preparation of biologically active analogs of serum low density lipoprotein.
A method for the preparation of stable and water-soluble analogs of low density lipoprotein (LDL) is presented. The experimental protocols start with the preparation of a cholesteryl ester/phospholipid microemulsion by a combined injection-sonication procedure and delipidation of apoprotein B (apoB)...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1984-06-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520377671 |
| _version_ | 1818726964008058880 |
|---|---|
| author | B Lundberg L Suominen |
| author_facet | B Lundberg L Suominen |
| author_sort | B Lundberg |
| collection | DOAJ |
| description | A method for the preparation of stable and water-soluble analogs of low density lipoprotein (LDL) is presented. The experimental protocols start with the preparation of a cholesteryl ester/phospholipid microemulsion by a combined injection-sonication procedure and delipidation of apoprotein B (apoB) with sodium deoxycholate (NaDOC). The association of lipid microemulsion and NaDOC-solubilized apoB is achieved by incubation and sonication of the components above the melting point of the cholesteryl ester. The reconstituted model LDL (m-LDL) proved to be quite homogeneous both with respect to particle size and composition. Negative-stain electron microscopy shows spherical particles with a mean diameter of 21 nm. The mean density of the reconstituted LDL was 1.07 g/ml as determined by sucrose density gradient centrifugation. The reconstituted LDL retained its beta-mobility on agarose gel electrophoresis, and sodium dodecyl sulfate (SDS)-gel electrophoresis showed no degradation of apoB during the reconstitution procedures. Studies of biological activity showed that the m-LDL particles are bound, incorporated, and degraded by human fibroblasts in a way similar to native LDL. The reconstituted m-LDL has potential use for metabolic, physiochemical, and enzymatic studies of lipoproteins. |
| first_indexed | 2024-12-17T22:06:34Z |
| format | Article |
| id | doaj.art-2a3aff7764054d1d9279fcfb6320db4c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:06:34Z |
| publishDate | 1984-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-2a3aff7764054d1d9279fcfb6320db4c2022-12-21T21:30:51ZengElsevierJournal of Lipid Research0022-22751984-06-01256550558Preparation of biologically active analogs of serum low density lipoprotein.B LundbergL SuominenA method for the preparation of stable and water-soluble analogs of low density lipoprotein (LDL) is presented. The experimental protocols start with the preparation of a cholesteryl ester/phospholipid microemulsion by a combined injection-sonication procedure and delipidation of apoprotein B (apoB) with sodium deoxycholate (NaDOC). The association of lipid microemulsion and NaDOC-solubilized apoB is achieved by incubation and sonication of the components above the melting point of the cholesteryl ester. The reconstituted model LDL (m-LDL) proved to be quite homogeneous both with respect to particle size and composition. Negative-stain electron microscopy shows spherical particles with a mean diameter of 21 nm. The mean density of the reconstituted LDL was 1.07 g/ml as determined by sucrose density gradient centrifugation. The reconstituted LDL retained its beta-mobility on agarose gel electrophoresis, and sodium dodecyl sulfate (SDS)-gel electrophoresis showed no degradation of apoB during the reconstitution procedures. Studies of biological activity showed that the m-LDL particles are bound, incorporated, and degraded by human fibroblasts in a way similar to native LDL. The reconstituted m-LDL has potential use for metabolic, physiochemical, and enzymatic studies of lipoproteins.http://www.sciencedirect.com/science/article/pii/S0022227520377671 |
| spellingShingle | B Lundberg L Suominen Preparation of biologically active analogs of serum low density lipoprotein. Journal of Lipid Research |
| title | Preparation of biologically active analogs of serum low density lipoprotein. |
| title_full | Preparation of biologically active analogs of serum low density lipoprotein. |
| title_fullStr | Preparation of biologically active analogs of serum low density lipoprotein. |
| title_full_unstemmed | Preparation of biologically active analogs of serum low density lipoprotein. |
| title_short | Preparation of biologically active analogs of serum low density lipoprotein. |
| title_sort | preparation of biologically active analogs of serum low density lipoprotein |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520377671 |
| work_keys_str_mv | AT blundberg preparationofbiologicallyactiveanalogsofserumlowdensitylipoprotein AT lsuominen preparationofbiologicallyactiveanalogsofserumlowdensitylipoprotein |