Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.
Slick (Slo2.1) and Slack (Slo2.2) channels belong to the family of high-conductance K+ channels and have been found widely distributed in the CNS. Both channels are activated by Na+ and Cl- and, in addition, Slick channels are regulated by ATP. Therefore, the roles of these channels in regulation of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4210196?pdf=render |
| _version_ | 1830516291268509696 |
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| author | Maria A Tejada Kathleen Stople Sofia Hammami Bomholtz Anne-Kristine Meinild Asser Nyander Poulsen Dan A Klaerke |
| author_facet | Maria A Tejada Kathleen Stople Sofia Hammami Bomholtz Anne-Kristine Meinild Asser Nyander Poulsen Dan A Klaerke |
| author_sort | Maria A Tejada |
| collection | DOAJ |
| description | Slick (Slo2.1) and Slack (Slo2.2) channels belong to the family of high-conductance K+ channels and have been found widely distributed in the CNS. Both channels are activated by Na+ and Cl- and, in addition, Slick channels are regulated by ATP. Therefore, the roles of these channels in regulation of cell excitability as well as ion transport processes, like regulation of cell volume, have been hypothesized. It is the aim of this work to evaluate the sensitivity of Slick and Slack channels to small, fast changes in cell volume and to explore mechanisms, which may explain this type of regulation. For this purpose Slick and Slack channels were co-expressed with aquaporin 1 in Xenopus laevis oocytes and cell volume changes of around 5% were induced by exposure to hypotonic or hypertonic media. Whole-cell currents were measured by two electrode voltage clamp. Our results show that Slick channels are dramatically stimulated (196% of control) by cell swelling and inhibited (57% of control) by a decrease in cell volume. In contrast, Slack channels are totally insensitive to similar cell volume changes. The mechanism underlining the strong volume sensitivity of Slick channels needs to be further explored, however we were able to show that it does not depend on an intact actin cytoskeleton, ATP release or vesicle fusion. In conclusion, Slick channels, in contrast to the similar Slack channels, are the only high-conductance K+ channels strongly sensitive to small changes in cell volume. |
| first_indexed | 2024-12-22T03:38:19Z |
| format | Article |
| id | doaj.art-2a3b095c8aa3406c962f0e2cd49202be |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:38:19Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2a3b095c8aa3406c962f0e2cd49202be2022-12-21T18:40:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11083310.1371/journal.pone.0110833Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels.Maria A TejadaKathleen StopleSofia Hammami BomholtzAnne-Kristine MeinildAsser Nyander PoulsenDan A KlaerkeSlick (Slo2.1) and Slack (Slo2.2) channels belong to the family of high-conductance K+ channels and have been found widely distributed in the CNS. Both channels are activated by Na+ and Cl- and, in addition, Slick channels are regulated by ATP. Therefore, the roles of these channels in regulation of cell excitability as well as ion transport processes, like regulation of cell volume, have been hypothesized. It is the aim of this work to evaluate the sensitivity of Slick and Slack channels to small, fast changes in cell volume and to explore mechanisms, which may explain this type of regulation. For this purpose Slick and Slack channels were co-expressed with aquaporin 1 in Xenopus laevis oocytes and cell volume changes of around 5% were induced by exposure to hypotonic or hypertonic media. Whole-cell currents were measured by two electrode voltage clamp. Our results show that Slick channels are dramatically stimulated (196% of control) by cell swelling and inhibited (57% of control) by a decrease in cell volume. In contrast, Slack channels are totally insensitive to similar cell volume changes. The mechanism underlining the strong volume sensitivity of Slick channels needs to be further explored, however we were able to show that it does not depend on an intact actin cytoskeleton, ATP release or vesicle fusion. In conclusion, Slick channels, in contrast to the similar Slack channels, are the only high-conductance K+ channels strongly sensitive to small changes in cell volume.http://europepmc.org/articles/PMC4210196?pdf=render |
| spellingShingle | Maria A Tejada Kathleen Stople Sofia Hammami Bomholtz Anne-Kristine Meinild Asser Nyander Poulsen Dan A Klaerke Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. PLoS ONE |
| title | Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. |
| title_full | Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. |
| title_fullStr | Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. |
| title_full_unstemmed | Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. |
| title_short | Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2) K+ channels. |
| title_sort | cell volume changes regulate slick slo2 1 but not slack slo2 2 k channels |
| url | http://europepmc.org/articles/PMC4210196?pdf=render |
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