Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers
For the fast and sensitive determination of camel's and goat's meat and milk, species-specific regions (SSR) of follicle stimulating hormone receptor (FSHR) gene in both camel and goat were amplified using PCR technique. DNA was extracted from small amount of muscles (0.05 gm) and very lit...
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| Format: | Article |
| Language: | English |
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Institute for Animal Husbandry, Belgrade
2015-01-01
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| Series: | Biotechnology in Animal Husbandry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2015/1450-91561503415A.pdf |
| _version_ | 1818139079655555072 |
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| author | Abdel-Rahman S.M. Elmaghraby A.M. Haggag A. S. |
| author_facet | Abdel-Rahman S.M. Elmaghraby A.M. Haggag A. S. |
| author_sort | Abdel-Rahman S.M. |
| collection | DOAJ |
| description | For the fast and sensitive determination of camel's and goat's meat and milk, species-specific regions (SSR) of follicle stimulating hormone receptor (FSHR) gene in both camel and goat were amplified using PCR technique. DNA was extracted from small amount of muscles (0.05 gm) and very little of fresh milk (100 μl) to amplify specific DNA sequences of FSHR gene in both camel and goat using designed species-specific primer pairs. PCR amplified fragment size was 300 bp in camel's meat and milk, while the fragment size in goat's meat and milk was 855 bp. The use of species-specific regions of FSHR gene allowed direct and fast detection of adulteration and authentication of camel's and goat's meat and milk. |
| first_indexed | 2024-12-11T10:22:23Z |
| format | Article |
| id | doaj.art-2a3cb3bb89f7475aa6630634c1c965d6 |
| institution | Directory Open Access Journal |
| issn | 1450-9156 2217-7140 |
| language | English |
| last_indexed | 2024-12-11T10:22:23Z |
| publishDate | 2015-01-01 |
| publisher | Institute for Animal Husbandry, Belgrade |
| record_format | Article |
| series | Biotechnology in Animal Husbandry |
| spelling | doaj.art-2a3cb3bb89f7475aa6630634c1c965d62022-12-22T01:11:21ZengInstitute for Animal Husbandry, BelgradeBiotechnology in Animal Husbandry1450-91562217-71402015-01-013134154231450-91561503415AFast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markersAbdel-Rahman S.M.0Elmaghraby A.M.1Haggag A. S.2Department of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, EgyptDepartment of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, EgyptDepartment of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, EgyptFor the fast and sensitive determination of camel's and goat's meat and milk, species-specific regions (SSR) of follicle stimulating hormone receptor (FSHR) gene in both camel and goat were amplified using PCR technique. DNA was extracted from small amount of muscles (0.05 gm) and very little of fresh milk (100 μl) to amplify specific DNA sequences of FSHR gene in both camel and goat using designed species-specific primer pairs. PCR amplified fragment size was 300 bp in camel's meat and milk, while the fragment size in goat's meat and milk was 855 bp. The use of species-specific regions of FSHR gene allowed direct and fast detection of adulteration and authentication of camel's and goat's meat and milk.https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2015/1450-91561503415A.pdfMeatmilkspecies-specificgenetic markersPCR |
| spellingShingle | Abdel-Rahman S.M. Elmaghraby A.M. Haggag A. S. Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers Biotechnology in Animal Husbandry Meat milk species-specific genetic markers PCR |
| title | Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers |
| title_full | Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers |
| title_fullStr | Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers |
| title_full_unstemmed | Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers |
| title_short | Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers |
| title_sort | fast and sensitive determination of camel s and goat s meat and milk using species specific genetic markers |
| topic | Meat milk species-specific genetic markers PCR |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2015/1450-91561503415A.pdf |
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