Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers
Both nitric oxide (NO) and calcium ion (Ca2+)/calmodulin (CaM) have been shown to regulate the senescence of cut flowers. However, not much is known about the crosstalk between NO and Ca2+/CaM during the senescence of cut flowers. In this study, cut Oriental × Trumpet hybrid lily “Manissa” were used...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-09-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01284/full |
| _version_ | 1818980796244951040 |
|---|---|
| author | Jing Zhang Hua Fang Jianqiang Huo Dengjing Huang Bo Wang Weibiao Liao |
| author_facet | Jing Zhang Hua Fang Jianqiang Huo Dengjing Huang Bo Wang Weibiao Liao |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Both nitric oxide (NO) and calcium ion (Ca2+)/calmodulin (CaM) have been shown to regulate the senescence of cut flowers. However, not much is known about the crosstalk between NO and Ca2+/CaM during the senescence of cut flowers. In this study, cut Oriental × Trumpet hybrid lily “Manissa” were used to investigate the roles and relationship between NO and Ca2+/CaM during postharvest freshness. The results show that the effects of CaCl2 or NO donor SNAP on the vase life, maximum flower diameter and hours until full opening were dose-dependent, with an optimum concentration of 20 mM CaCl2 or 100 μM SNAP. However, Ca2+ chelators EGTA or BAPTA/AM, Ca2+ channel inhibitors LaCl3 or nifedipine and CaM antagonists W-7 or TFP inhibited the promotion of SNAP. SNAP applied alone significantly increased the endogenous Ca2+/CaM contents in cut lily flowers, while EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP decreased the advancement of SNAP. In addition, the SNAP-induced Ca2+-ATPase activity was more than twice as much as the control, but EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP also reversed the enhancement. Moreover, EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP prevented the SNAP-induced upregulation of gene expression of CaM, CBL1, and CBL3, which is associated with calcium signaling pathway. Overall, these results suggest that Ca2+/CaM may function as downstream molecules in NO-regulated senescence of cut flowers. |
| first_indexed | 2024-12-20T17:21:07Z |
| format | Article |
| id | doaj.art-2a3d84d8b8ba480e936198ba12f6714a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T17:21:07Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2a3d84d8b8ba480e936198ba12f6714a2022-12-21T19:31:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-09-01910.3389/fpls.2018.01284398079Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily FlowersJing ZhangHua FangJianqiang HuoDengjing HuangBo WangWeibiao LiaoBoth nitric oxide (NO) and calcium ion (Ca2+)/calmodulin (CaM) have been shown to regulate the senescence of cut flowers. However, not much is known about the crosstalk between NO and Ca2+/CaM during the senescence of cut flowers. In this study, cut Oriental × Trumpet hybrid lily “Manissa” were used to investigate the roles and relationship between NO and Ca2+/CaM during postharvest freshness. The results show that the effects of CaCl2 or NO donor SNAP on the vase life, maximum flower diameter and hours until full opening were dose-dependent, with an optimum concentration of 20 mM CaCl2 or 100 μM SNAP. However, Ca2+ chelators EGTA or BAPTA/AM, Ca2+ channel inhibitors LaCl3 or nifedipine and CaM antagonists W-7 or TFP inhibited the promotion of SNAP. SNAP applied alone significantly increased the endogenous Ca2+/CaM contents in cut lily flowers, while EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP decreased the advancement of SNAP. In addition, the SNAP-induced Ca2+-ATPase activity was more than twice as much as the control, but EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP also reversed the enhancement. Moreover, EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP prevented the SNAP-induced upregulation of gene expression of CaM, CBL1, and CBL3, which is associated with calcium signaling pathway. Overall, these results suggest that Ca2+/CaM may function as downstream molecules in NO-regulated senescence of cut flowers.https://www.frontiersin.org/article/10.3389/fpls.2018.01284/fullpostharvest freshnesscalcium ion/calmodulinCa2+-ATPasegene expressioncalcium signaling |
| spellingShingle | Jing Zhang Hua Fang Jianqiang Huo Dengjing Huang Bo Wang Weibiao Liao Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers Frontiers in Plant Science postharvest freshness calcium ion/calmodulin Ca2+-ATPase gene expression calcium signaling |
| title | Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers |
| title_full | Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers |
| title_fullStr | Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers |
| title_full_unstemmed | Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers |
| title_short | Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers |
| title_sort | involvement of calcium and calmodulin in nitric oxide regulated senescence of cut lily flowers |
| topic | postharvest freshness calcium ion/calmodulin Ca2+-ATPase gene expression calcium signaling |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01284/full |
| work_keys_str_mv | AT jingzhang involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers AT huafang involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers AT jianqianghuo involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers AT dengjinghuang involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers AT bowang involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers AT weibiaoliao involvementofcalciumandcalmodulininnitricoxideregulatedsenescenceofcutlilyflowers |