Evaluation of the TGS TA system for the detection of anti-rubella antibodies
<p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and I...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2017-07-01
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| Series: | Microbiologia Medica |
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| Online Access: | http://www.pagepressjournals.org/index.php/mm/article/view/6582 |
| _version_ | 1818001217323794432 |
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| author | Olivia Arpino Annalisa Cianflone Maria Teresa Manco Alessia Paganini Massimo De Paschale Carlo Agrappi Paola Mirri Pierangelo Clerici |
| author_facet | Olivia Arpino Annalisa Cianflone Maria Teresa Manco Alessia Paganini Massimo De Paschale Carlo Agrappi Paola Mirri Pierangelo Clerici |
| author_sort | Olivia Arpino |
| collection | DOAJ |
| description | <p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study), TGS TA system was compared to an ELFA IgM test and with an ELISA test for the IgG avidity (if existent).<br /><em>Materials and methods:</em> Two hundred and seventy six sera samples from women were examined with TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 112 were of childbearing age and non-immune women (Group 1), 106 were pregnant with past infection or vaccinated (Group 2) and 49 were pregnant with positive or equivocal IgM (Group 3). <br /><em>Results</em>: The overall concordance of the IgG results between LIAISON XL and TGS TA was 93.3%: 86.6% in Group 1, 97.2% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 89.0%: 100% in Group 1, 100% in Group 2 and 35.6% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELISA and TGS TA tests was 85.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 97.4%: 86.6% in Group 1, 97.2% in Group 2 and 85.7% in Group 3. <br /><em>Conclusions</em>: TGA TS system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection or those who are vaccinated. The TGS TA test also seems to be especially sensitive in indicating vaccinated subjects with low IgG levels as immune.</p> |
| first_indexed | 2024-04-14T03:31:40Z |
| format | Article |
| id | doaj.art-2a42c1f6c01d4041a009299ba7042f32 |
| institution | Directory Open Access Journal |
| issn | 2280-6423 |
| language | English |
| last_indexed | 2024-04-14T03:31:40Z |
| publishDate | 2017-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj.art-2a42c1f6c01d4041a009299ba7042f322022-12-22T02:14:55ZengPAGEPress PublicationsMicrobiologia Medica2280-64232017-07-0132210.4081/mm.2017.65825328Evaluation of the TGS TA system for the detection of anti-rubella antibodiesOlivia Arpino0Annalisa Cianflone1Maria Teresa Manco2Alessia Paganini3Massimo De Paschale4Carlo Agrappi5Paola Mirri6Pierangelo Clerici7Microbiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, LegnanoMicrobiology Unit, Legnano Hospital, ASST Ovest Milanese, Legnano<p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study), TGS TA system was compared to an ELFA IgM test and with an ELISA test for the IgG avidity (if existent).<br /><em>Materials and methods:</em> Two hundred and seventy six sera samples from women were examined with TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 112 were of childbearing age and non-immune women (Group 1), 106 were pregnant with past infection or vaccinated (Group 2) and 49 were pregnant with positive or equivocal IgM (Group 3). <br /><em>Results</em>: The overall concordance of the IgG results between LIAISON XL and TGS TA was 93.3%: 86.6% in Group 1, 97.2% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 89.0%: 100% in Group 1, 100% in Group 2 and 35.6% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELISA and TGS TA tests was 85.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 97.4%: 86.6% in Group 1, 97.2% in Group 2 and 85.7% in Group 3. <br /><em>Conclusions</em>: TGA TS system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection or those who are vaccinated. The TGS TA test also seems to be especially sensitive in indicating vaccinated subjects with low IgG levels as immune.</p>http://www.pagepressjournals.org/index.php/mm/article/view/6582RubellaPregnancyIgMIgGAvidity |
| spellingShingle | Olivia Arpino Annalisa Cianflone Maria Teresa Manco Alessia Paganini Massimo De Paschale Carlo Agrappi Paola Mirri Pierangelo Clerici Evaluation of the TGS TA system for the detection of anti-rubella antibodies Microbiologia Medica Rubella Pregnancy IgM IgG Avidity |
| title | Evaluation of the TGS TA system for the detection of anti-rubella antibodies |
| title_full | Evaluation of the TGS TA system for the detection of anti-rubella antibodies |
| title_fullStr | Evaluation of the TGS TA system for the detection of anti-rubella antibodies |
| title_full_unstemmed | Evaluation of the TGS TA system for the detection of anti-rubella antibodies |
| title_short | Evaluation of the TGS TA system for the detection of anti-rubella antibodies |
| title_sort | evaluation of the tgs ta system for the detection of anti rubella antibodies |
| topic | Rubella Pregnancy IgM IgG Avidity |
| url | http://www.pagepressjournals.org/index.php/mm/article/view/6582 |
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