Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study
Background: Whole-slide imaging (WSI) has been implemented in many areas of pathology, but primary diagnostics of cytological specimens are lagging behind. One of the objectives of viewing scanned whole-slide images from histological or cytological specimens is remote exchange of knowledge and exper...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2018;volume=9;issue=1;spage=29;epage=29;aulast=Bongaerts |
| _version_ | 1818265060911349760 |
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| author | Odille Bongaerts Carla Clevers Marij Debets Daniëlle Paffen Lisanne Senden Kim Rijks Linda Ruiten Daisy Sie-Go Paul J van Diest Marius Nap |
| author_facet | Odille Bongaerts Carla Clevers Marij Debets Daniëlle Paffen Lisanne Senden Kim Rijks Linda Ruiten Daisy Sie-Go Paul J van Diest Marius Nap |
| author_sort | Odille Bongaerts |
| collection | DOAJ |
| description | Background: Whole-slide imaging (WSI) has been implemented in many areas of pathology, but primary diagnostics of cytological specimens are lagging behind. One of the objectives of viewing scanned whole-slide images from histological or cytological specimens is remote exchange of knowledge and expertise of professionals to increase diagnostic accuracy. We compared the scoring results of our team obtained in double readings of two different data sets: conventional light microscopy (CLM) versus CLM and CLM versus WSI. We hypothesized that WSI is noninferior to CLM for primary diagnostics of thin-layer cervical slides. Materials and Methods: First, we determined the concordance rate at different thresholds of the participating cytotechnicians by double reading with CLM of 500 thin-layer cervical slides (Cohort 1). Next, CLM was compared with WSI examination of another 505 thin-layer cervical slides (Cohort 2) scanned at ×20 in single focus plane. Finally, all major discordant cases of Cohort 1 were evaluated by an external expert in the field of gynecological cytology and of Cohort 2 in the weekly case meetings. Results: The overall concordance rate of Cohort 1 (CLM vs. CLM) was 97.8% (95% confidence interval [CI]: 96.0%–98.7%) and of Cohort 2 was 95.3% (95% CI: 93.0%–96.9%). Conclusion: Concordance rates of WSI versus CLM were comparable with those of CLM versus CLM. We have made a step forward paving the road to implementation of WSI also in routine diagnostic cytology. |
| first_indexed | 2024-12-12T19:44:48Z |
| format | Article |
| id | doaj.art-2a47263120ef44198ffe1ec52ce0103b |
| institution | Directory Open Access Journal |
| issn | 2153-3539 2153-3539 |
| language | English |
| last_indexed | 2024-12-12T19:44:48Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-2a47263120ef44198ffe1ec52ce0103b2022-12-22T00:14:07ZengElsevierJournal of Pathology Informatics2153-35392153-35392018-01-0191292910.4103/jpi.jpi_28_18Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation studyOdille BongaertsCarla CleversMarij DebetsDaniëlle PaffenLisanne SendenKim RijksLinda RuitenDaisy Sie-GoPaul J van DiestMarius NapBackground: Whole-slide imaging (WSI) has been implemented in many areas of pathology, but primary diagnostics of cytological specimens are lagging behind. One of the objectives of viewing scanned whole-slide images from histological or cytological specimens is remote exchange of knowledge and expertise of professionals to increase diagnostic accuracy. We compared the scoring results of our team obtained in double readings of two different data sets: conventional light microscopy (CLM) versus CLM and CLM versus WSI. We hypothesized that WSI is noninferior to CLM for primary diagnostics of thin-layer cervical slides. Materials and Methods: First, we determined the concordance rate at different thresholds of the participating cytotechnicians by double reading with CLM of 500 thin-layer cervical slides (Cohort 1). Next, CLM was compared with WSI examination of another 505 thin-layer cervical slides (Cohort 2) scanned at ×20 in single focus plane. Finally, all major discordant cases of Cohort 1 were evaluated by an external expert in the field of gynecological cytology and of Cohort 2 in the weekly case meetings. Results: The overall concordance rate of Cohort 1 (CLM vs. CLM) was 97.8% (95% confidence interval [CI]: 96.0%–98.7%) and of Cohort 2 was 95.3% (95% CI: 93.0%–96.9%). Conclusion: Concordance rates of WSI versus CLM were comparable with those of CLM versus CLM. We have made a step forward paving the road to implementation of WSI also in routine diagnostic cytology.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2018;volume=9;issue=1;spage=29;epage=29;aulast=BongaertsCervical cytologycytologydigital pathologyvalidation studywhole-slide imaging |
| spellingShingle | Odille Bongaerts Carla Clevers Marij Debets Daniëlle Paffen Lisanne Senden Kim Rijks Linda Ruiten Daisy Sie-Go Paul J van Diest Marius Nap Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study Journal of Pathology Informatics Cervical cytology cytology digital pathology validation study whole-slide imaging |
| title | Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study |
| title_full | Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study |
| title_fullStr | Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study |
| title_full_unstemmed | Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study |
| title_short | Conventional microscopical versus digital whole-slide imaging-based diagnosis of thin-layer cervical specimens: A validation study |
| title_sort | conventional microscopical versus digital whole slide imaging based diagnosis of thin layer cervical specimens a validation study |
| topic | Cervical cytology cytology digital pathology validation study whole-slide imaging |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2018;volume=9;issue=1;spage=29;epage=29;aulast=Bongaerts |
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