Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants
<p>Abstract</p> <p>Background</p> <p>Highly active anti-retroviral therapy (HAART) is the current HIV/AIDS treatment modality. Despite the fact that HAART is very effective in suppressing HIV-1 replication and reducing the mortality of HIV/AIDS patients, it has become i...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-08-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/9/29 |
| _version_ | 1831769797884379136 |
|---|---|
| author | Song Xinming Cen Changchun Liu Ying Wang Ting Green Linden A Song Xiaoping Han Changri Park In-Woo Yang Biao Chen Guangying He Johnny J |
| author_facet | Song Xinming Cen Changchun Liu Ying Wang Ting Green Linden A Song Xiaoping Han Changri Park In-Woo Yang Biao Chen Guangying He Johnny J |
| author_sort | Song Xinming |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Highly active anti-retroviral therapy (HAART) is the current HIV/AIDS treatment modality. Despite the fact that HAART is very effective in suppressing HIV-1 replication and reducing the mortality of HIV/AIDS patients, it has become increasingly clear that HAART does not offer an ultimate cure to HIV/AIDS. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. This need has further manifested by recent clinical trial failures in anti-HIV-1 vaccines and microbicides. In the current study, we characterized a panel of extracts of traditional Chinese medicinal herbal plants for their activities against HIV-1 replication.</p> <p>Methods</p> <p>Crude and fractionated extracts were prepared from various parts of nine traditional Chinese medicinal herbal plants in Hainan Island, China. These extracts were first screened for their anti-HIV activity and cytotoxicity in human CD4+ Jurkat cells. Then, a single-round pseudotyped HIV-luciferase reporter virus system (HIV-Luc) was used to identify potential anti-HIV mechanisms of these extracts.</p> <p>Results</p> <p>Two extracts, one from <it>Euphorbiaceae</it>, <it>Trigonostema xyphophylloides </it>(TXE) and one from <it>Dipterocarpaceae</it>, <it>Vatica astrotricha </it>(VAD) inhibited HIV-1 replication and syncytia formation in CD4+ Jurkat cells, and had little adverse effects on host cell proliferation and survival. TXE and VAD did not show any direct inhibitory effects on the HIV-1 RT enzymatic activity. Treatment of these two extracts during the infection significantly blocked infection of the reporter virus. However, pre-treatment of the reporter virus with the extracts and treatment of the extracts post-infection had little effects on the infectivity or gene expression of the reporter virus.</p> <p>Conclusion</p> <p>These results demonstrate that TXE and VAD inhibit HIV-1 replication likely by blocking HIV-1 interaction with target cells, i.e., the interaction between gp120 and CD4/CCR5 or gp120 and CD4/CXCR4 and point to the potential of developing these two extracts to be HIV-1 entry inhibitors.</p> |
| first_indexed | 2024-12-22T07:28:02Z |
| format | Article |
| id | doaj.art-2a4810914e6444449b0ee205eb9e9fa3 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T07:28:02Z |
| publishDate | 2009-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-2a4810914e6444449b0ee205eb9e9fa32022-12-21T18:34:06ZengBMCBMC Complementary and Alternative Medicine1472-68822009-08-01912910.1186/1472-6882-9-29Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plantsSong XinmingCen ChangchunLiu YingWang TingGreen Linden ASong XiaopingHan ChangriPark In-WooYang BiaoChen GuangyingHe Johnny J<p>Abstract</p> <p>Background</p> <p>Highly active anti-retroviral therapy (HAART) is the current HIV/AIDS treatment modality. Despite the fact that HAART is very effective in suppressing HIV-1 replication and reducing the mortality of HIV/AIDS patients, it has become increasingly clear that HAART does not offer an ultimate cure to HIV/AIDS. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. This need has further manifested by recent clinical trial failures in anti-HIV-1 vaccines and microbicides. In the current study, we characterized a panel of extracts of traditional Chinese medicinal herbal plants for their activities against HIV-1 replication.</p> <p>Methods</p> <p>Crude and fractionated extracts were prepared from various parts of nine traditional Chinese medicinal herbal plants in Hainan Island, China. These extracts were first screened for their anti-HIV activity and cytotoxicity in human CD4+ Jurkat cells. Then, a single-round pseudotyped HIV-luciferase reporter virus system (HIV-Luc) was used to identify potential anti-HIV mechanisms of these extracts.</p> <p>Results</p> <p>Two extracts, one from <it>Euphorbiaceae</it>, <it>Trigonostema xyphophylloides </it>(TXE) and one from <it>Dipterocarpaceae</it>, <it>Vatica astrotricha </it>(VAD) inhibited HIV-1 replication and syncytia formation in CD4+ Jurkat cells, and had little adverse effects on host cell proliferation and survival. TXE and VAD did not show any direct inhibitory effects on the HIV-1 RT enzymatic activity. Treatment of these two extracts during the infection significantly blocked infection of the reporter virus. However, pre-treatment of the reporter virus with the extracts and treatment of the extracts post-infection had little effects on the infectivity or gene expression of the reporter virus.</p> <p>Conclusion</p> <p>These results demonstrate that TXE and VAD inhibit HIV-1 replication likely by blocking HIV-1 interaction with target cells, i.e., the interaction between gp120 and CD4/CCR5 or gp120 and CD4/CXCR4 and point to the potential of developing these two extracts to be HIV-1 entry inhibitors.</p>http://www.biomedcentral.com/1472-6882/9/29 |
| spellingShingle | Song Xinming Cen Changchun Liu Ying Wang Ting Green Linden A Song Xiaoping Han Changri Park In-Woo Yang Biao Chen Guangying He Johnny J Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants BMC Complementary and Alternative Medicine |
| title | Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants |
| title_full | Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants |
| title_fullStr | Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants |
| title_full_unstemmed | Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants |
| title_short | Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants |
| title_sort | inhibition of hiv 1 entry by extracts derived from traditional chinese medicinal herbal plants |
| url | http://www.biomedcentral.com/1472-6882/9/29 |
| work_keys_str_mv | AT songxinming inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT cenchangchun inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT liuying inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT wangting inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT greenlindena inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT songxiaoping inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT hanchangri inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT parkinwoo inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT yangbiao inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT chenguangying inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants AT hejohnnyj inhibitionofhiv1entrybyextractsderivedfromtraditionalchinesemedicinalherbalplants |