Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia
Fecal contamination with a poor water, sanitation and hygiene environment in urban informal settlements poses diarrhea risks. Little information is available on the contamination of environmental media with enteric pathogens in such settlements. We investigated the contamination of Escherichia coli,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-07-01
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| Series: | Journal of Water and Health |
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| Online Access: | http://jwh.iwaponline.com/content/20/7/1027 |
| _version_ | 1818172620280954880 |
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| author | Yasuhiro Asada Min Li Chua Mayu Tsurumi Taro Yamauchi Imasiku Nyambe Hidenori Harada |
| author_facet | Yasuhiro Asada Min Li Chua Mayu Tsurumi Taro Yamauchi Imasiku Nyambe Hidenori Harada |
| author_sort | Yasuhiro Asada |
| collection | DOAJ |
| description | Fecal contamination with a poor water, sanitation and hygiene environment in urban informal settlements poses diarrhea risks. Little information is available on the contamination of environmental media with enteric pathogens in such settlements. We investigated the contamination of Escherichia coli, rotavirus, and Cryptosporidium spp. in water, on kitchenware, and on flies in urban informal settlements of Chawama and Kanyama, Lusaka, Zambia. These environmental media were examined by XM-G agar cultivation for E. coli and specific real-time RT-PCR assays to detect rotavirus and Cryptosporidium spp. E. coli; rotavirus, and Cryptosporidium spp. were detected in samples of household stored drinking water (6 of 10 samples, 3 of 10 samples, and 2 of 10 samples, respectively), cups (10 of 20 samples, 2 of 13 samples, 1 of 13 samples, respectively), and flies (35 of 55 samples, 5 of 17 samples, 1 of 17 samples, respectively). The ranges of rotavirus concentrations in household stored drinking water, on cups, and flies were 2.9 × 102–2.2 × 105 copies/L, 1.2 × 102–4.3 × 102 copies/cup, and 5.0 × 101–2.0 × 102 copies/fly, respectively. These results indicate the contribution of drinking water and kitchenware to enteric pathogen exposure and potential role of flies in microbial transmission. HIGHLIGHTS
The study was conducted in a periurban informal settlement of sub-Saharan Africa.;
Rotavirus and Cryptosporidium spp. were detected from point-of-use drinking water.;
These two pathogens were also detected from drinking cups and flies.;
People are exposed to these enteric pathogens from drinking water as well as cups.;
The results imply the role of flies as a vector of these enteric pathogens.; |
| first_indexed | 2024-12-11T19:15:30Z |
| format | Article |
| id | doaj.art-2a4a7cef69684aa7b94245a574394567 |
| institution | Directory Open Access Journal |
| issn | 1477-8920 1996-7829 |
| language | English |
| last_indexed | 2024-12-11T19:15:30Z |
| publishDate | 2022-07-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Journal of Water and Health |
| spelling | doaj.art-2a4a7cef69684aa7b94245a5743945672022-12-22T00:53:40ZengIWA PublishingJournal of Water and Health1477-89201996-78292022-07-012071027103710.2166/wh.2022.276276Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, ZambiaYasuhiro Asada0Min Li Chua1Mayu Tsurumi2Taro Yamauchi3Imasiku Nyambe4Hidenori Harada5 Department of Environmental Health, National Institute of Public Health, Saitama, Japan Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan Graduate School of Global Environmental Studies, Kyoto University, Kyoto, Japan Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan Integrated Water Resources Management Centre, c/o School of Mines, University of Zambia, Lusaka, Zambia Research Institute for Humanity and Nature, Kyoto, Japan Fecal contamination with a poor water, sanitation and hygiene environment in urban informal settlements poses diarrhea risks. Little information is available on the contamination of environmental media with enteric pathogens in such settlements. We investigated the contamination of Escherichia coli, rotavirus, and Cryptosporidium spp. in water, on kitchenware, and on flies in urban informal settlements of Chawama and Kanyama, Lusaka, Zambia. These environmental media were examined by XM-G agar cultivation for E. coli and specific real-time RT-PCR assays to detect rotavirus and Cryptosporidium spp. E. coli; rotavirus, and Cryptosporidium spp. were detected in samples of household stored drinking water (6 of 10 samples, 3 of 10 samples, and 2 of 10 samples, respectively), cups (10 of 20 samples, 2 of 13 samples, 1 of 13 samples, respectively), and flies (35 of 55 samples, 5 of 17 samples, 1 of 17 samples, respectively). The ranges of rotavirus concentrations in household stored drinking water, on cups, and flies were 2.9 × 102–2.2 × 105 copies/L, 1.2 × 102–4.3 × 102 copies/cup, and 5.0 × 101–2.0 × 102 copies/fly, respectively. These results indicate the contribution of drinking water and kitchenware to enteric pathogen exposure and potential role of flies in microbial transmission. HIGHLIGHTS The study was conducted in a periurban informal settlement of sub-Saharan Africa.; Rotavirus and Cryptosporidium spp. were detected from point-of-use drinking water.; These two pathogens were also detected from drinking cups and flies.; People are exposed to these enteric pathogens from drinking water as well as cups.; The results imply the role of flies as a vector of these enteric pathogens.;http://jwh.iwaponline.com/content/20/7/1027cryptosporidium spp.e. colilusakarotavirusurban informal settlementswash |
| spellingShingle | Yasuhiro Asada Min Li Chua Mayu Tsurumi Taro Yamauchi Imasiku Nyambe Hidenori Harada Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia Journal of Water and Health cryptosporidium spp. e. coli lusaka rotavirus urban informal settlements wash |
| title | Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia |
| title_full | Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia |
| title_fullStr | Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia |
| title_full_unstemmed | Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia |
| title_short | Detection of Escherichia coli, rotavirus, and Cryptosporidium spp. from drinking water, kitchenware, and flies in a periurban community of Lusaka, Zambia |
| title_sort | detection of escherichia coli rotavirus and cryptosporidium spp from drinking water kitchenware and flies in a periurban community of lusaka zambia |
| topic | cryptosporidium spp. e. coli lusaka rotavirus urban informal settlements wash |
| url | http://jwh.iwaponline.com/content/20/7/1027 |
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