The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins
The attachment of enteropathogenic <i>Escherichia coli</i> (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10350 |
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| author | Rajesh Mondal Zeus Saldaña-Ahuactzi Jorge Soria-Bustos Andrew Schultz Jorge A. Yañez-Santos Ygnacio Martínez Laguna María L. Cedillo-Ramírez Jorge A. Girón |
| author_facet | Rajesh Mondal Zeus Saldaña-Ahuactzi Jorge Soria-Bustos Andrew Schultz Jorge A. Yañez-Santos Ygnacio Martínez Laguna María L. Cedillo-Ramírez Jorge A. Girón |
| author_sort | Rajesh Mondal |
| collection | DOAJ |
| description | The attachment of enteropathogenic <i>Escherichia coli</i> (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that the <i>E. coli</i> common pilus (ECP) tip adhesin protein EcpD mediates attachment of EPEC to several extracellular matrix (ECM) glycoproteins (fibronectin, laminin, collagens I and IV, and mucin). We found that the Δ<i>ecpA</i> mutant, which lacks production of the EcpA filament but retains EcpD on the surface, adhered to these glycoproteins below the wild-type levels, while the Δ<i>ecpD</i> mutant, which does not display EcpA or EcpD, bound significantly less to these host glycoproteins. In agreement, a purified recombinant EcpD subunit bound significantly more than EcpA to laminin, fibronectin, collagens I and IV, and mucin in a dose-dependent manner. These are compelling data that strongly suggest that ECP-producing EPEC may bind to host ECM glycoproteins and mucins through the tip adhesin protein EcpD. This study highlights the versatility of EPEC to bind to different host proteins and suggests that the interaction of ECP with the host’s ECM glycoproteins may facilitate colonization of the intestinal mucosal epithelium. |
| first_indexed | 2024-03-09T23:47:30Z |
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| id | doaj.art-2a4c6d9617e6451eb8c92d396b448816 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:47:30Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2a4c6d9617e6451eb8c92d396b4488162023-11-23T16:40:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181035010.3390/ijms231810350The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix ProteinsRajesh Mondal0Zeus Saldaña-Ahuactzi1Jorge Soria-Bustos2Andrew Schultz3Jorge A. Yañez-Santos4Ygnacio Martínez Laguna5María L. Cedillo-Ramírez6Jorge A. Girón7ICMR-Bhopal Memorial Hospital and Research Center, Bhopal 462038, IndiaPaul G. Allen School for Global Health, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexicali 21100, MexicoDepartment of Microbiology and Molecular Genetics, University of Florida, Gainesville, FL 32611, USAFacultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla 72592, MexicoCentro de Investigación en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Puebla 72592, MexicoCentro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla 72592, MexicoCentro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla 72592, MexicoThe attachment of enteropathogenic <i>Escherichia coli</i> (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that the <i>E. coli</i> common pilus (ECP) tip adhesin protein EcpD mediates attachment of EPEC to several extracellular matrix (ECM) glycoproteins (fibronectin, laminin, collagens I and IV, and mucin). We found that the Δ<i>ecpA</i> mutant, which lacks production of the EcpA filament but retains EcpD on the surface, adhered to these glycoproteins below the wild-type levels, while the Δ<i>ecpD</i> mutant, which does not display EcpA or EcpD, bound significantly less to these host glycoproteins. In agreement, a purified recombinant EcpD subunit bound significantly more than EcpA to laminin, fibronectin, collagens I and IV, and mucin in a dose-dependent manner. These are compelling data that strongly suggest that ECP-producing EPEC may bind to host ECM glycoproteins and mucins through the tip adhesin protein EcpD. This study highlights the versatility of EPEC to bind to different host proteins and suggests that the interaction of ECP with the host’s ECM glycoproteins may facilitate colonization of the intestinal mucosal epithelium.https://www.mdpi.com/1422-0067/23/18/10350ECPfibronectinECMpilusEPEC |
| spellingShingle | Rajesh Mondal Zeus Saldaña-Ahuactzi Jorge Soria-Bustos Andrew Schultz Jorge A. Yañez-Santos Ygnacio Martínez Laguna María L. Cedillo-Ramírez Jorge A. Girón The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins International Journal of Molecular Sciences ECP fibronectin ECM pilus EPEC |
| title | The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins |
| title_full | The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins |
| title_fullStr | The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins |
| title_full_unstemmed | The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins |
| title_short | The EcpD Tip Adhesin of the <i>Escherichia coli</i> Common Pilus Mediates Binding of Enteropathogenic <i>E. coli</i> to Extracellular Matrix Proteins |
| title_sort | ecpd tip adhesin of the i escherichia coli i common pilus mediates binding of enteropathogenic i e coli i to extracellular matrix proteins |
| topic | ECP fibronectin ECM pilus EPEC |
| url | https://www.mdpi.com/1422-0067/23/18/10350 |
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