Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
Commensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellone...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2020.1848107 |
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| author | Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li |
| author_facet | Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li |
| author_sort | Xiao-Wen Huang |
| collection | DOAJ |
| description | Commensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellonella infection model, we were able to confirm the much lower virulence of C. glabrata than C. albicans. Interestingly, pre-exposure to live C. glabrata (LCG) protects the larvae against subsequent various lethal fungal infections, including C. albicans, Candida tropicalis, and Cryptococcus neoformans. Inconsistently, heat-inactivated C. glabrata (HICG) pre-exposure can only protect against C. albicans or C. tropicalis re-infection, but not C. neoformans. Mechanistically, LCG or HICG pre-exposure enhanced the fungicidal activity of hemocytes against C. albicans or C. tropicalis. Meanwhile, LCG pre-exposure enhanced the humoral immunity by modulating the expression of fungal defending proteins in the cell-free hemolymph, which may contribute to the protection against C. neoformans. Together, this study suggests the important role of C. glabrata in enhancing host immunity, and demonstrates the great potential of G. mellonella model in studying the innate immune responses against infections. |
| first_indexed | 2024-12-22T01:50:45Z |
| format | Article |
| id | doaj.art-2a4d6e29faac4331b3123d977e752a5b |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-22T01:50:45Z |
| publishDate | 2020-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-2a4d6e29faac4331b3123d977e752a5b2022-12-21T18:42:56ZengTaylor & Francis GroupVirulence2150-55942150-56082020-12-011111674168410.1080/21505594.2020.18481071848107Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infectionsXiao-Wen Huang0Mei-Nian Xu1Huan-Xin Zheng2Meng-Lei Wang3Li Li4Kang Zeng5De-Dong Li6Southern Medical UniversitySouthern Medical UniversitySouthern Medical UniversitySouthern Medical UniversitySouthern Medical UniversitySouthern Medical UniversityUniversity of PittsburghCommensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellonella infection model, we were able to confirm the much lower virulence of C. glabrata than C. albicans. Interestingly, pre-exposure to live C. glabrata (LCG) protects the larvae against subsequent various lethal fungal infections, including C. albicans, Candida tropicalis, and Cryptococcus neoformans. Inconsistently, heat-inactivated C. glabrata (HICG) pre-exposure can only protect against C. albicans or C. tropicalis re-infection, but not C. neoformans. Mechanistically, LCG or HICG pre-exposure enhanced the fungicidal activity of hemocytes against C. albicans or C. tropicalis. Meanwhile, LCG pre-exposure enhanced the humoral immunity by modulating the expression of fungal defending proteins in the cell-free hemolymph, which may contribute to the protection against C. neoformans. Together, this study suggests the important role of C. glabrata in enhancing host immunity, and demonstrates the great potential of G. mellonella model in studying the innate immune responses against infections.http://dx.doi.org/10.1080/21505594.2020.1848107candida glabratagalleria mellonellainnate immunityhemocytesimmune priming |
| spellingShingle | Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections Virulence candida glabrata galleria mellonella innate immunity hemocytes immune priming |
| title | Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections |
| title_full | Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections |
| title_fullStr | Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections |
| title_full_unstemmed | Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections |
| title_short | Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections |
| title_sort | pre exposure to candida glabrata protects galleria mellonella against subsequent lethal fungal infections |
| topic | candida glabrata galleria mellonella innate immunity hemocytes immune priming |
| url | http://dx.doi.org/10.1080/21505594.2020.1848107 |
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