Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae)
<i>Lycium minutifolium</i> J. Remy (Solanaceae) is commonly used as an infusion in traditional medicine to treat stomach pain, meteorism, intestinal disorders, stomach ailments, and other severe problems including prostate cancer and stomach cancer. From the EtOAc extract of <i>L....
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/5/565 |
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| author | Stephanie Rodriguez Mariano Walter Pertino Chantal Arcos Luana Reichert Javier Echeverria Mario Simirgiotis Jorge Borquez Alberto Cornejo Carlos Areche Beatriz Sepulveda |
| author_facet | Stephanie Rodriguez Mariano Walter Pertino Chantal Arcos Luana Reichert Javier Echeverria Mario Simirgiotis Jorge Borquez Alberto Cornejo Carlos Areche Beatriz Sepulveda |
| author_sort | Stephanie Rodriguez |
| collection | DOAJ |
| description | <i>Lycium minutifolium</i> J. Remy (Solanaceae) is commonly used as an infusion in traditional medicine to treat stomach pain, meteorism, intestinal disorders, stomach ailments, and other severe problems including prostate cancer and stomach cancer. From the EtOAc extract of <i>L. minutifolium</i> bark five known metabolites were isolated using chromatographic techniques. The gastroprotective effects of the EtOAc fraction and edible infusion extract of the bark were assayed on the hydrochloric acid (HCl)/EtOH induced gastric ulcer model in mice to support the traditional use of the plant. The EtOAc extract and the edible infusion showed gastroprotective effect at dose of 100 mg/kg reducing lesions by 31% and 64%, respectively. The gastroprotective action mechanisms of the edible infusion at a single oral dose of 100 mg/kg were evaluated suggesting that prostaglandins, sulfhydryl groups, and nitric oxide are involved in the mode of gastroprotective action. The UHPLC analysis coupled to high-resolution mass spectrometry of the edible infusion showed the presence of twenty-three compounds. Our results can support the gastroprotective properties of the edible infusion extract, and at least can validate in part, the ethnopharmacological uses of the plant. |
| first_indexed | 2024-03-10T20:03:12Z |
| format | Article |
| id | doaj.art-2a53de0f00224704ae5523c0b87255d7 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T20:03:12Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2a53de0f00224704ae5523c0b87255d72023-11-19T23:25:34ZengMDPI AGFoods2304-81582020-05-019556510.3390/foods9050565Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae)Stephanie Rodriguez0Mariano Walter Pertino1Chantal Arcos2Luana Reichert3Javier Echeverria4Mario Simirgiotis5Jorge Borquez6Alberto Cornejo7Carlos Areche8Beatriz Sepulveda9Departamento de Química, Facultad de Ciencias, Universidad de Chile, 8320000 Santiago, ChileLaboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, 3460000 Talca, ChileDepartamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar, 2531098 Valparaiso, ChileDepartamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar, 2531098 Valparaiso, ChileDepartamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, 9170022 Santiago, ChileInstituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, 5090000 Valdivia, ChileDepartamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av Coloso S-N, 1240000 Antofagasta, ChileEscuela de Tecnología Médica, Facultad de Medicina, Universidad Andres Bello, Sazié 2315, 8370092 Santiago, ChileDepartamento de Química, Facultad de Ciencias, Universidad de Chile, 8320000 Santiago, ChileDepartamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar, 2531098 Valparaiso, Chile<i>Lycium minutifolium</i> J. Remy (Solanaceae) is commonly used as an infusion in traditional medicine to treat stomach pain, meteorism, intestinal disorders, stomach ailments, and other severe problems including prostate cancer and stomach cancer. From the EtOAc extract of <i>L. minutifolium</i> bark five known metabolites were isolated using chromatographic techniques. The gastroprotective effects of the EtOAc fraction and edible infusion extract of the bark were assayed on the hydrochloric acid (HCl)/EtOH induced gastric ulcer model in mice to support the traditional use of the plant. The EtOAc extract and the edible infusion showed gastroprotective effect at dose of 100 mg/kg reducing lesions by 31% and 64%, respectively. The gastroprotective action mechanisms of the edible infusion at a single oral dose of 100 mg/kg were evaluated suggesting that prostaglandins, sulfhydryl groups, and nitric oxide are involved in the mode of gastroprotective action. The UHPLC analysis coupled to high-resolution mass spectrometry of the edible infusion showed the presence of twenty-three compounds. Our results can support the gastroprotective properties of the edible infusion extract, and at least can validate in part, the ethnopharmacological uses of the plant.https://www.mdpi.com/2304-8158/9/5/565coumarins<i>Lycium</i>metabolomicHPLC-MSorbitrapsecondary metabolites |
| spellingShingle | Stephanie Rodriguez Mariano Walter Pertino Chantal Arcos Luana Reichert Javier Echeverria Mario Simirgiotis Jorge Borquez Alberto Cornejo Carlos Areche Beatriz Sepulveda Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) Foods coumarins <i>Lycium</i> metabolomic HPLC-MS orbitrap secondary metabolites |
| title | Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) |
| title_full | Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) |
| title_fullStr | Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) |
| title_full_unstemmed | Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) |
| title_short | Isolation, Gastroprotective Effects and Untargeted Metabolomics Analysis of <i>Lycium Minutifolium</i> J. Remy (Solanaceae) |
| title_sort | isolation gastroprotective effects and untargeted metabolomics analysis of i lycium minutifolium i j remy solanaceae |
| topic | coumarins <i>Lycium</i> metabolomic HPLC-MS orbitrap secondary metabolites |
| url | https://www.mdpi.com/2304-8158/9/5/565 |
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