Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas)
Trehalose plays an important role in energy storage, metabolism, and protection from extreme environmental conditions in insects. Trehalose is the main blood sugar in insects, and it can be rapidly used as an energy source in times of need. To elucidate the mechanisms of the starvation response, we...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2017-07-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/6/7/1096 |
| _version_ | 1818865583627698176 |
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| author | Zuo-Kun Shi Su Wang Shi-Gui Wang Lu Zhang Yan-Xia Xu Xiao-Jun Guo Fan Zhang Bin Tang |
| author_facet | Zuo-Kun Shi Su Wang Shi-Gui Wang Lu Zhang Yan-Xia Xu Xiao-Jun Guo Fan Zhang Bin Tang |
| author_sort | Zuo-Kun Shi |
| collection | DOAJ |
| description | Trehalose plays an important role in energy storage, metabolism, and protection from extreme environmental conditions in insects. Trehalose is the main blood sugar in insects, and it can be rapidly used as an energy source in times of need. To elucidate the mechanisms of the starvation response, we observed the effects of starvation on trehalose and glycogen, trehalase activity, and the relative gene expression of genes in the trehalose and glycogen metabolic pathways in the invasive beetle Harmonia axyridis. Our results show that trehalose levels and the activities of two types of trehalases decreased significantly in the first 8 h of starvation, while the relative expression of HaTreh1-1 increased. While trehalose remained nearly constant at a relatively high level from 8 to 24 h, glycogen levels decreased significantly from 8 h to 24 h of starvation. Likewise, glycogen phosphorylase (HaGP) expression was significantly higher at 12 to 24 h starvation than the first 8 h, while the expression of glycogen synthase (HaGS) was relatively stable. Furthermore, trehalose decreased significantly from 24 h starvation to 72 h starvation, while trehalase activities and the relative expression of some HaTreh genes generally increased toward the end of the starvation period. The expression of trehalose-6-phosphate synthase (HaTPS) increased significantly, supporting the increase in trehalose synthesis. These results show that trehalose plays a key role in the energy provided during the starvation process through the molecular and biochemical regulation of trehalose and glycogen metabolism. |
| first_indexed | 2024-12-19T10:49:52Z |
| format | Article |
| id | doaj.art-2a567ab6735e4f8dbdc184e88ac0c361 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T10:49:52Z |
| publishDate | 2017-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-2a567ab6735e4f8dbdc184e88ac0c3612022-12-21T20:25:03ZengThe Company of BiologistsBiology Open2046-63902017-07-01671096110310.1242/bio.025189025189Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas)Zuo-Kun Shi0Su Wang1Shi-Gui Wang2Lu Zhang3Yan-Xia Xu4Xiao-Jun Guo5Fan Zhang6Bin Tang7 Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China Trehalose plays an important role in energy storage, metabolism, and protection from extreme environmental conditions in insects. Trehalose is the main blood sugar in insects, and it can be rapidly used as an energy source in times of need. To elucidate the mechanisms of the starvation response, we observed the effects of starvation on trehalose and glycogen, trehalase activity, and the relative gene expression of genes in the trehalose and glycogen metabolic pathways in the invasive beetle Harmonia axyridis. Our results show that trehalose levels and the activities of two types of trehalases decreased significantly in the first 8 h of starvation, while the relative expression of HaTreh1-1 increased. While trehalose remained nearly constant at a relatively high level from 8 to 24 h, glycogen levels decreased significantly from 8 h to 24 h of starvation. Likewise, glycogen phosphorylase (HaGP) expression was significantly higher at 12 to 24 h starvation than the first 8 h, while the expression of glycogen synthase (HaGS) was relatively stable. Furthermore, trehalose decreased significantly from 24 h starvation to 72 h starvation, while trehalase activities and the relative expression of some HaTreh genes generally increased toward the end of the starvation period. The expression of trehalose-6-phosphate synthase (HaTPS) increased significantly, supporting the increase in trehalose synthesis. These results show that trehalose plays a key role in the energy provided during the starvation process through the molecular and biochemical regulation of trehalose and glycogen metabolism.http://bio.biologists.org/content/6/7/1096TrehalaseTrehaloseGlycogenStarvationQRT-PCRHarmonia axyridis |
| spellingShingle | Zuo-Kun Shi Su Wang Shi-Gui Wang Lu Zhang Yan-Xia Xu Xiao-Jun Guo Fan Zhang Bin Tang Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) Biology Open Trehalase Trehalose Glycogen Starvation QRT-PCR Harmonia axyridis |
| title | Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) |
| title_full | Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) |
| title_fullStr | Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) |
| title_full_unstemmed | Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) |
| title_short | Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas) |
| title_sort | effects of starvation on the carbohydrate metabolism in harmonia axyridis pallas |
| topic | Trehalase Trehalose Glycogen Starvation QRT-PCR Harmonia axyridis |
| url | http://bio.biologists.org/content/6/7/1096 |
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