MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma
AbstractRecent evidence has implicated microRNA-219 (miR-219) in regulation of gene contributed in glioblastoma (GBM) pathogenesis. This study aimed to prepare miR-219 in chitosan (CS) nanoparticles (NPs), characterize and investigate their efficacy on human GBM cell line (U87 MG). NPs were prepared...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2022.2092123 |
| _version_ | 1798003809563181056 |
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| author | Rawan Alswailem Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Bahauddeen M. Alrfaei Mohammad Badran Qamraa Hamad Alqahtani Hosam Gharib Abdelhady Ibrahim Alsarra |
| author_facet | Rawan Alswailem Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Bahauddeen M. Alrfaei Mohammad Badran Qamraa Hamad Alqahtani Hosam Gharib Abdelhady Ibrahim Alsarra |
| author_sort | Rawan Alswailem |
| collection | DOAJ |
| description | AbstractRecent evidence has implicated microRNA-219 (miR-219) in regulation of gene contributed in glioblastoma (GBM) pathogenesis. This study aimed to prepare miR-219 in chitosan (CS) nanoparticles (NPs), characterize and investigate their efficacy on human GBM cell line (U87 MG). NPs were prepared using ionic gelation method. The influence of process parameters on physicochemical characteristics of NPs was investigated. Apoptotic effect of miR-219 was examined on U87 MG cells. Formulated NPs showed particle size of 109 ± 2.18 nm, with poly dispersity index equal to 0.2 ± 0.05, and zeta potential of +20.5 ± 0.7 mV. Entrapment efficiency of miR-219 in loaded NP has reached 95%. The in vitro release study demonstrated sustained release pattern of miR-219 from CS-NPs. Gel retardation assay has confirmed the integrity of miR-219 after production process. The fabricated NPs reduced the survival of U87 MG cells to 78% after 24 h of post-transfection, and into 67.5% after 48 h. However, fibroblasts were not affected by the NPs, revealing their specificity for GBM cells. Given the tumour suppressing function of miR-219, and advantage of CS-NPs for gene delivery to the central nervous system, the presented NPs have a great potential for treatment of GBM. |
| first_indexed | 2024-04-11T12:14:43Z |
| format | Article |
| id | doaj.art-2a5a3f7cb0dc4278b83a01882ea8227c |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-11T12:14:43Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-2a5a3f7cb0dc4278b83a01882ea8227c2022-12-22T04:24:24ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2022-12-0150119820710.1080/21691401.2022.2092123MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastomaRawan Alswailem0Fulwah Yahya Alqahtani1Fadilah Sfouq Aleanizy2Bahauddeen M. Alrfaei3Mohammad Badran4Qamraa Hamad Alqahtani5Hosam Gharib Abdelhady6Ibrahim Alsarra7Drug sector, Saudi Food and Drug Authority, Riyadh, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Cellular Therapy and Cancer Research, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health, Riyadh, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaCollege of Osteopathic Medicine, Sam Houston State University, Conroe, TX, USADepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaAbstractRecent evidence has implicated microRNA-219 (miR-219) in regulation of gene contributed in glioblastoma (GBM) pathogenesis. This study aimed to prepare miR-219 in chitosan (CS) nanoparticles (NPs), characterize and investigate their efficacy on human GBM cell line (U87 MG). NPs were prepared using ionic gelation method. The influence of process parameters on physicochemical characteristics of NPs was investigated. Apoptotic effect of miR-219 was examined on U87 MG cells. Formulated NPs showed particle size of 109 ± 2.18 nm, with poly dispersity index equal to 0.2 ± 0.05, and zeta potential of +20.5 ± 0.7 mV. Entrapment efficiency of miR-219 in loaded NP has reached 95%. The in vitro release study demonstrated sustained release pattern of miR-219 from CS-NPs. Gel retardation assay has confirmed the integrity of miR-219 after production process. The fabricated NPs reduced the survival of U87 MG cells to 78% after 24 h of post-transfection, and into 67.5% after 48 h. However, fibroblasts were not affected by the NPs, revealing their specificity for GBM cells. Given the tumour suppressing function of miR-219, and advantage of CS-NPs for gene delivery to the central nervous system, the presented NPs have a great potential for treatment of GBM.https://www.tandfonline.com/doi/10.1080/21691401.2022.2092123GlioblastomamicroRNAChitosanNanoparticlesgene deliverymicroRNA-219 |
| spellingShingle | Rawan Alswailem Fulwah Yahya Alqahtani Fadilah Sfouq Aleanizy Bahauddeen M. Alrfaei Mohammad Badran Qamraa Hamad Alqahtani Hosam Gharib Abdelhady Ibrahim Alsarra MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma Artificial Cells, Nanomedicine, and Biotechnology Glioblastoma microRNA Chitosan Nanoparticles gene delivery microRNA-219 |
| title | MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma |
| title_full | MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma |
| title_fullStr | MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma |
| title_full_unstemmed | MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma |
| title_short | MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma |
| title_sort | microrna 219 loaded chitosan nanoparticles for treatment of glioblastoma |
| topic | Glioblastoma microRNA Chitosan Nanoparticles gene delivery microRNA-219 |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2022.2092123 |
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