Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces
Applying antibacterial coatings to dental implant materials seems reasonable but can have negative influences on desired cell adhesion and healing. In this study, zirconia abutment specimens interacting with gingival tissue were used. The aim was to compare the influence of machined or coated zircon...
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| author | Oskar Bunz Marie-Christine Steegmann Korbinian Benz Holger Testrich Antje Quade Ella A. Naumova Wolfgang H. Arnold Katja Fricke Andree Piwowarczyk Thomas Dittmar |
| author_facet | Oskar Bunz Marie-Christine Steegmann Korbinian Benz Holger Testrich Antje Quade Ella A. Naumova Wolfgang H. Arnold Katja Fricke Andree Piwowarczyk Thomas Dittmar |
| author_sort | Oskar Bunz |
| collection | DOAJ |
| description | Applying antibacterial coatings to dental implant materials seems reasonable but can have negative influences on desired cell adhesion and healing. In this study, zirconia abutment specimens interacting with gingival tissue were used. The aim was to compare the influence of machined or coated zirconia surfaces on the adhesion and proliferation of human gingival fibroblasts (HGF-1). Surface modifications were performed using atmospheric plasma coating with hydroxyapatite, zinc, and copper. Zirconia specimens were divided into four groups: hydroxyapatite, hydroxyapatite with zinc oxide (ZnO), hydroxyapatite with copper (Cu), and an untreated machined surface. After the characterization of the surface conditions, the morphology of adhered HGF-1 was determined by fluorescence staining and subjected to statistical evaluation. The visual analysis of cell morphology by SEM showed flat, polygonal, and largely adherent fibroblast cells in the untreated group, while round to partially flat cells were recorded in the groups with hydroxyapatite, hydroxyapatite + ZnO, and hydroxyapatite + Cu. The cell membranes in the hydroxyapatite + ZnO and hydroxyapatite + Cu groups appeared porous. The results show that HGF-1 adhere and proliferate well on machined zirconia, while plasma coating with hydroxyapatite or hydroxyapatite mixtures does not lead to increased adhesion or proliferation. |
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| format | Article |
| id | doaj.art-2a5c9cf7690b4f519c0993ba0e93465b |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T03:29:39Z |
| publishDate | 2022-05-01 |
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| series | Materials |
| spelling | doaj.art-2a5c9cf7690b4f519c0993ba0e93465b2023-11-23T11:58:13ZengMDPI AGMaterials1996-19442022-05-011510362510.3390/ma15103625Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment SurfacesOskar Bunz0Marie-Christine Steegmann1Korbinian Benz2Holger Testrich3Antje Quade4Ella A. Naumova5Wolfgang H. Arnold6Katja Fricke7Andree Piwowarczyk8Thomas Dittmar9Department of Prosthodontics, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, GermanyIndependent Researcher, 55116 Mainz, GermanyDepartment of Oral Surgery and Dental Emergency Care, Faculty of Health, Witten/Herdecke University, 58455 Witten, GermanyResearch Group of Bioactive Surfaces, INP Greifswald e.V., 17489 Greifswald, GermanyResearch Group of Bioactive Surfaces, INP Greifswald e.V., 17489 Greifswald, GermanyDepartment of Biological and Material Sciences in Dentistry, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, GermanyDepartment of Biological and Material Sciences in Dentistry, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, GermanyResearch Group of Bioactive Surfaces, INP Greifswald e.V., 17489 Greifswald, GermanyDepartment of Prosthodontics, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, GermanyInstitute of Immunology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, GermanyApplying antibacterial coatings to dental implant materials seems reasonable but can have negative influences on desired cell adhesion and healing. In this study, zirconia abutment specimens interacting with gingival tissue were used. The aim was to compare the influence of machined or coated zirconia surfaces on the adhesion and proliferation of human gingival fibroblasts (HGF-1). Surface modifications were performed using atmospheric plasma coating with hydroxyapatite, zinc, and copper. Zirconia specimens were divided into four groups: hydroxyapatite, hydroxyapatite with zinc oxide (ZnO), hydroxyapatite with copper (Cu), and an untreated machined surface. After the characterization of the surface conditions, the morphology of adhered HGF-1 was determined by fluorescence staining and subjected to statistical evaluation. The visual analysis of cell morphology by SEM showed flat, polygonal, and largely adherent fibroblast cells in the untreated group, while round to partially flat cells were recorded in the groups with hydroxyapatite, hydroxyapatite + ZnO, and hydroxyapatite + Cu. The cell membranes in the hydroxyapatite + ZnO and hydroxyapatite + Cu groups appeared porous. The results show that HGF-1 adhere and proliferate well on machined zirconia, while plasma coating with hydroxyapatite or hydroxyapatite mixtures does not lead to increased adhesion or proliferation.https://www.mdpi.com/1996-1944/15/10/3625ceramic implantatmospheric plasma sprayingabutmenthuman gingival fibroblasts |
| spellingShingle | Oskar Bunz Marie-Christine Steegmann Korbinian Benz Holger Testrich Antje Quade Ella A. Naumova Wolfgang H. Arnold Katja Fricke Andree Piwowarczyk Thomas Dittmar Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces Materials ceramic implant atmospheric plasma spraying abutment human gingival fibroblasts |
| title | Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces |
| title_full | Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces |
| title_fullStr | Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces |
| title_full_unstemmed | Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces |
| title_short | Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces |
| title_sort | human gingival fibroblast adhesion and proliferation on hydroxyapatite coated zirconia abutment surfaces |
| topic | ceramic implant atmospheric plasma spraying abutment human gingival fibroblasts |
| url | https://www.mdpi.com/1996-1944/15/10/3625 |
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