How different microfilters affect the recovery of eleven EU-regulated mycotoxins
Microfiltration is a common step in liquid chromatography – tandem mass spectrometry (LC-MS), a method of choice in determining several mycotoxins in a solution at once. However, microfiltration may entail filter-analyte interactions that can affect the accuracy of the procedure, and underestimate e...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-03-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
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| Online Access: | https://doi.org/10.2478/aiht-2023-74-3693 |
| _version_ | 1797810835083493376 |
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| author | Tomas Marija Kovač Mijatović Anto Nevistić Mateja Bulaić Šarkanj Bojan Babić Jurislav Kovač Tihomir |
| author_facet | Tomas Marija Kovač Mijatović Anto Nevistić Mateja Bulaić Šarkanj Bojan Babić Jurislav Kovač Tihomir |
| author_sort | Tomas Marija Kovač |
| collection | DOAJ |
| description | Microfiltration is a common step in liquid chromatography – tandem mass spectrometry (LC-MS), a method of choice in determining several mycotoxins in a solution at once. However, microfiltration may entail filter-analyte interactions that can affect the accuracy of the procedure, and underestimate exposure. The aim of our study was to assess how five different membrane materials for syringe filters (nylon, polytetrafluoroethylene, polyethersulphone, mixed cellulose ester, and cellulose acetate) affect microfiltration and recovery of EU-regulated mycotoxins, including aflatoxins B1, B2, G1, and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A. Polytetrafluoroethylene filters turned out to least affect microfiltration through mycotoxin loss, followed by more commonly used nylon filters, whereas the remaining three filter membrane materials had such a negative effect on recoveries that we found them incompatible with the procedure. Our findings clearly suggest that it is important to select a proper filter type that suits analyte properties and solution composition and to discard the first few filtrate drops to ensure the accuracy of the analytical procedure. |
| first_indexed | 2024-03-13T07:14:46Z |
| format | Article |
| id | doaj.art-2a60114a9f2f4bbdaa57270fcfc2f9bb |
| institution | Directory Open Access Journal |
| issn | 1848-6312 |
| language | English |
| last_indexed | 2024-03-13T07:14:46Z |
| publishDate | 2023-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj.art-2a60114a9f2f4bbdaa57270fcfc2f9bb2023-06-05T09:18:20ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122023-03-0174181510.2478/aiht-2023-74-3693How different microfilters affect the recovery of eleven EU-regulated mycotoxinsTomas Marija Kovač0Mijatović Anto1Nevistić Mateja Bulaić2Šarkanj Bojan3Babić Jurislav4Kovač Tihomir51University North, Department of Food Technology, Koprivnica, Croatia1University North, Department of Food Technology, Koprivnica, Croatia3Inspecto Ltd., Osijek, Croatia1University North, Department of Food Technology, Koprivnica, Croatia4Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology, Osijek, Croatia4Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology, Osijek, CroatiaMicrofiltration is a common step in liquid chromatography – tandem mass spectrometry (LC-MS), a method of choice in determining several mycotoxins in a solution at once. However, microfiltration may entail filter-analyte interactions that can affect the accuracy of the procedure, and underestimate exposure. The aim of our study was to assess how five different membrane materials for syringe filters (nylon, polytetrafluoroethylene, polyethersulphone, mixed cellulose ester, and cellulose acetate) affect microfiltration and recovery of EU-regulated mycotoxins, including aflatoxins B1, B2, G1, and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A. Polytetrafluoroethylene filters turned out to least affect microfiltration through mycotoxin loss, followed by more commonly used nylon filters, whereas the remaining three filter membrane materials had such a negative effect on recoveries that we found them incompatible with the procedure. Our findings clearly suggest that it is important to select a proper filter type that suits analyte properties and solution composition and to discard the first few filtrate drops to ensure the accuracy of the analytical procedure.https://doi.org/10.2478/aiht-2023-74-3693filter-analyte interactionliquid chromatography-tandem mass spectrometryunderestimationsample preparationinterakcije filtar-analitpodcjenjivanje rezultatapriprema uzorkaregulirani mikotoksinitekućinska kromatografija – tandemska spektrometrija masa |
| spellingShingle | Tomas Marija Kovač Mijatović Anto Nevistić Mateja Bulaić Šarkanj Bojan Babić Jurislav Kovač Tihomir How different microfilters affect the recovery of eleven EU-regulated mycotoxins Arhiv za Higijenu Rada i Toksikologiju filter-analyte interaction liquid chromatography-tandem mass spectrometry underestimation sample preparation interakcije filtar-analit podcjenjivanje rezultata priprema uzorka regulirani mikotoksini tekućinska kromatografija – tandemska spektrometrija masa |
| title | How different microfilters affect the recovery of eleven EU-regulated mycotoxins |
| title_full | How different microfilters affect the recovery of eleven EU-regulated mycotoxins |
| title_fullStr | How different microfilters affect the recovery of eleven EU-regulated mycotoxins |
| title_full_unstemmed | How different microfilters affect the recovery of eleven EU-regulated mycotoxins |
| title_short | How different microfilters affect the recovery of eleven EU-regulated mycotoxins |
| title_sort | how different microfilters affect the recovery of eleven eu regulated mycotoxins |
| topic | filter-analyte interaction liquid chromatography-tandem mass spectrometry underestimation sample preparation interakcije filtar-analit podcjenjivanje rezultata priprema uzorka regulirani mikotoksini tekućinska kromatografija – tandemska spektrometrija masa |
| url | https://doi.org/10.2478/aiht-2023-74-3693 |
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