Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution
Collagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously coll...
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MDPI AG
2020-10-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/21/4698 |
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| author | Dalila Miele Laura Catenacci Silvia Rossi Giuseppina Sandri Milena Sorrenti Alberta Terzi Cinzia Giannini Federica Riva Franca Ferrari Carla Caramella Maria Cristina Bonferoni |
| author_facet | Dalila Miele Laura Catenacci Silvia Rossi Giuseppina Sandri Milena Sorrenti Alberta Terzi Cinzia Giannini Federica Riva Franca Ferrari Carla Caramella Maria Cristina Bonferoni |
| author_sort | Dalila Miele |
| collection | DOAJ |
| description | Collagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously collagen/PCL combinations were electrospun in solvents such as hexafluoroisopropanol (HFIP) or trifluoroethanol (TFE), more recently literature describes collagen/PCL nanofibers obtained in acidic aqueous solutions. A good morphology of the fibers represents in this case still a challenge, especially for high collagen/PCL ratios. In this work, thanks to preliminary rheological and physicochemical characterization of the solutions and to a Design of Experiments (DOE) approach on process parameters, regular and dimensionally uniform fibers were obtained with collagen/PCL ratios up to 1:2 and 1:1 w/w. Collagen ratio appeared relevant for mechanical strength of dry and hydrated fibers. WAXS and FTIR analysis showed that collagen denaturation is related both to the medium and to the electrospinning process. After one week in aqueous environment, collagen release was complete and a concentration dependent stimulatory effect on fibroblast growth was observed, suggesting the fiber suitability for wound healing. The positive effect of collagen on mechanical properties and on fibroblast biocompatibility was confirmed by a direct comparison of nanofiber performance after collagen substitution with gelatin. |
| first_indexed | 2024-03-10T15:25:23Z |
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| id | doaj.art-2a60dec37f764568911c856ec91e4b0a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T15:25:23Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-2a60dec37f764568911c856ec91e4b0a2023-11-20T18:03:21ZengMDPI AGMaterials1996-19442020-10-011321469810.3390/ma13214698Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen ContributionDalila Miele0Laura Catenacci1Silvia Rossi2Giuseppina Sandri3Milena Sorrenti4Alberta Terzi5Cinzia Giannini6Federica Riva7Franca Ferrari8Carla Caramella9Maria Cristina Bonferoni10Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyInstitute of Crystallography, CNR-IC, Via Amendola 122/O, 70126 Bari, ItalyInstitute of Crystallography, CNR-IC, Via Amendola 122/O, 70126 Bari, ItalyDepartment of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 2, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, ItalyCollagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously collagen/PCL combinations were electrospun in solvents such as hexafluoroisopropanol (HFIP) or trifluoroethanol (TFE), more recently literature describes collagen/PCL nanofibers obtained in acidic aqueous solutions. A good morphology of the fibers represents in this case still a challenge, especially for high collagen/PCL ratios. In this work, thanks to preliminary rheological and physicochemical characterization of the solutions and to a Design of Experiments (DOE) approach on process parameters, regular and dimensionally uniform fibers were obtained with collagen/PCL ratios up to 1:2 and 1:1 w/w. Collagen ratio appeared relevant for mechanical strength of dry and hydrated fibers. WAXS and FTIR analysis showed that collagen denaturation is related both to the medium and to the electrospinning process. After one week in aqueous environment, collagen release was complete and a concentration dependent stimulatory effect on fibroblast growth was observed, suggesting the fiber suitability for wound healing. The positive effect of collagen on mechanical properties and on fibroblast biocompatibility was confirmed by a direct comparison of nanofiber performance after collagen substitution with gelatin.https://www.mdpi.com/1996-1944/13/21/4698electrospun nanofiberscollagenpolycaprolactonedesign of experiments |
| spellingShingle | Dalila Miele Laura Catenacci Silvia Rossi Giuseppina Sandri Milena Sorrenti Alberta Terzi Cinzia Giannini Federica Riva Franca Ferrari Carla Caramella Maria Cristina Bonferoni Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution Materials electrospun nanofibers collagen polycaprolactone design of experiments |
| title | Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution |
| title_full | Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution |
| title_fullStr | Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution |
| title_full_unstemmed | Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution |
| title_short | Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution |
| title_sort | collagen pcl nanofibers electrospun in green solvent by doe assisted process an insight into collagen contribution |
| topic | electrospun nanofibers collagen polycaprolactone design of experiments |
| url | https://www.mdpi.com/1996-1944/13/21/4698 |
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