Adjuvants influence the maturation of VRC01-like antibodies during immunization
Summary: Once naive B cells expressing germline VRC01-class B cell receptors become activated by germline-targeting immunogens, they enter germinal centers and undergo affinity maturation. Booster immunizations with heterologous Envs are required for the full maturation of VRC01-class antibodies. He...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422201745X |
| _version_ | 1798019266364047360 |
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| author | Maria L. Knudsen Parul Agrawal Anna MacCamy K. Rachael Parks Matthew D. Gray Brittany N. Takushi Arineh Khechaduri Kelsey R. Salladay Rhea N. Coler Celia C. LaBranche David Montefiori Leonidas Stamatatos |
| author_facet | Maria L. Knudsen Parul Agrawal Anna MacCamy K. Rachael Parks Matthew D. Gray Brittany N. Takushi Arineh Khechaduri Kelsey R. Salladay Rhea N. Coler Celia C. LaBranche David Montefiori Leonidas Stamatatos |
| author_sort | Maria L. Knudsen |
| collection | DOAJ |
| description | Summary: Once naive B cells expressing germline VRC01-class B cell receptors become activated by germline-targeting immunogens, they enter germinal centers and undergo affinity maturation. Booster immunizations with heterologous Envs are required for the full maturation of VRC01-class antibodies. Here, we examined whether and how three adjuvants, Poly(I:C), GLA-LSQ, or Rehydragel, that activate different pathways of the innate immune system, influence the rate and type of somatic mutations accumulated by VRC01-class BCRs that become activated by the germline-targeting 426c.Mod.Core immunogen and the heterologous HxB2.WT.Core booster immunogen. We report that although the adjuvant used had no influence on the durability of plasma antibody responses after the prime, it influenced the plasma VRC01 antibody titers after the boost and the accumulation of somatic mutations on the elicited VRC01 antibodies. |
| first_indexed | 2024-04-11T16:38:03Z |
| format | Article |
| id | doaj.art-2a64a437ba714d9bb9830002de24fd76 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T16:38:03Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2a64a437ba714d9bb9830002de24fd762022-12-22T04:13:45ZengElsevieriScience2589-00422022-11-012511105473Adjuvants influence the maturation of VRC01-like antibodies during immunizationMaria L. Knudsen0Parul Agrawal1Anna MacCamy2K. Rachael Parks3Matthew D. Gray4Brittany N. Takushi5Arineh Khechaduri6Kelsey R. Salladay7Rhea N. Coler8Celia C. LaBranche9David Montefiori10Leonidas Stamatatos11Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA; Department of Global Health, University of Washington, Seattle, WA 98195, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USADepartment of Global Health, University of Washington, Seattle, WA 98195, USA; Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA; Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USADivision of Surgical Sciences, Duke University, Durham, NC 27710, USADivision of Surgical Sciences, Duke University, Durham, NC 27710, USAVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA; Department of Global Health, University of Washington, Seattle, WA 98195, USA; Corresponding authorSummary: Once naive B cells expressing germline VRC01-class B cell receptors become activated by germline-targeting immunogens, they enter germinal centers and undergo affinity maturation. Booster immunizations with heterologous Envs are required for the full maturation of VRC01-class antibodies. Here, we examined whether and how three adjuvants, Poly(I:C), GLA-LSQ, or Rehydragel, that activate different pathways of the innate immune system, influence the rate and type of somatic mutations accumulated by VRC01-class BCRs that become activated by the germline-targeting 426c.Mod.Core immunogen and the heterologous HxB2.WT.Core booster immunogen. We report that although the adjuvant used had no influence on the durability of plasma antibody responses after the prime, it influenced the plasma VRC01 antibody titers after the boost and the accumulation of somatic mutations on the elicited VRC01 antibodies.http://www.sciencedirect.com/science/article/pii/S258900422201745XBiological sciencesImmune responseImmunologyVirology |
| spellingShingle | Maria L. Knudsen Parul Agrawal Anna MacCamy K. Rachael Parks Matthew D. Gray Brittany N. Takushi Arineh Khechaduri Kelsey R. Salladay Rhea N. Coler Celia C. LaBranche David Montefiori Leonidas Stamatatos Adjuvants influence the maturation of VRC01-like antibodies during immunization iScience Biological sciences Immune response Immunology Virology |
| title | Adjuvants influence the maturation of VRC01-like antibodies during immunization |
| title_full | Adjuvants influence the maturation of VRC01-like antibodies during immunization |
| title_fullStr | Adjuvants influence the maturation of VRC01-like antibodies during immunization |
| title_full_unstemmed | Adjuvants influence the maturation of VRC01-like antibodies during immunization |
| title_short | Adjuvants influence the maturation of VRC01-like antibodies during immunization |
| title_sort | adjuvants influence the maturation of vrc01 like antibodies during immunization |
| topic | Biological sciences Immune response Immunology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422201745X |
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