Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam
Tuberculosis (TB) has been a threat to world health for decades. Multidrug-resistant TB (MDR-TB) is adding to the burden of disease and hindering the development of countries. This study aimed to identify the drug-resistant rate in patients with recurrent pulmonary TB and detect rifampicin resistanc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Mahidol University
2022-05-01
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| Series: | Pharmaceutical Sciences Asia |
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| Online Access: | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-3_8.pdf |
| _version_ | 1818140858366558208 |
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| author | Loan Thi Duong Khang Tan Do Dung Nhan Tran Dung Ngoc Tran Phuc Huu Luu Thang Nguyen |
| author_facet | Loan Thi Duong Khang Tan Do Dung Nhan Tran Dung Ngoc Tran Phuc Huu Luu Thang Nguyen |
| author_sort | Loan Thi Duong |
| collection | DOAJ |
| description | Tuberculosis (TB) has been a threat to world health for decades. Multidrug-resistant TB (MDR-TB) is adding to the burden of disease and hindering the development of countries. This study aimed to identify the drug-resistant rate in patients with recurrent pulmonary TB and detect rifampicin resistance mutations in the rpoB gene in Can Tho, Vietnam. In this descriptive cross-sectional study, clinicians diagnosed the patients with recurrent pulmonary TB and were hospitalized. Smear-positive sputum specimens (n=246) were collected to determine the rate of drug resistance. To identify mutations in the rpoB gene which are likely to be associated with rifampicin resistance in MDR-TB, we sequenced 40 isolates of Mycobacterium tuberculosis collected between 2012 and 2014; mainly MDR-TB (n=40; 95.2%) and rifampicin-sensitive TB (n=2; 4.8%). The rate of drug-resistant pulmonary tuberculosis was 63.8%, and the rate of MDR-TB accounted for 23.2%. The mutations in rpoB were predominantly in codons 531 (27.5%) and 523 (17.5%), with rare occurrences of S522A (2.5%) and A532P (2.5%). Noticeably, there was a substitution mutation in codon 532, and a mutant strain of tuberculosis at seven codons in the rpoB gene had been detected. In conclusion, this study provided drug-resistant characteristics in patients with recurrent pulmonary TB and mutations of MDR-TB in Can Tho, Vietnam. The results show a mutation in codon 532 of the rpoB gene and a mutant strain of tuberculosis at all seven codons. These are remarkable and promising results for further studies and clinical applications.
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| first_indexed | 2024-12-11T10:50:40Z |
| format | Article |
| id | doaj.art-2a64d8f8539348b387942341cf457887 |
| institution | Directory Open Access Journal |
| issn | 2586-8470 |
| language | English |
| last_indexed | 2024-12-11T10:50:40Z |
| publishDate | 2022-05-01 |
| publisher | Faculty of Pharmacy, Mahidol University |
| record_format | Article |
| series | Pharmaceutical Sciences Asia |
| spelling | doaj.art-2a64d8f8539348b387942341cf4578872022-12-22T01:10:19ZengFaculty of Pharmacy, Mahidol UniversityPharmaceutical Sciences Asia2586-84702022-05-0149327328110.29090/psa.2022.03.21.232Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, VietnamLoan Thi Duong0Khang Tan Do1Dung Nhan Tran2Dung Ngoc Tran3Phuc Huu Luu4Thang Nguyen5Can Tho University of Medicine and Pharmacy, Can Tho City, VietnamBiotechnology Research and Development Institute, Can Tho City, VietnamBiotechnology Research and Development Institute, Can Tho City, VietnamCan Tho University of Medicine and Pharmacy, Can Tho City, VietnamCan Tho University of Medicine and Pharmacy, Can Tho City, VietnamCan Tho University of Medicine and Pharmacy, Can Tho City, VietnamTuberculosis (TB) has been a threat to world health for decades. Multidrug-resistant TB (MDR-TB) is adding to the burden of disease and hindering the development of countries. This study aimed to identify the drug-resistant rate in patients with recurrent pulmonary TB and detect rifampicin resistance mutations in the rpoB gene in Can Tho, Vietnam. In this descriptive cross-sectional study, clinicians diagnosed the patients with recurrent pulmonary TB and were hospitalized. Smear-positive sputum specimens (n=246) were collected to determine the rate of drug resistance. To identify mutations in the rpoB gene which are likely to be associated with rifampicin resistance in MDR-TB, we sequenced 40 isolates of Mycobacterium tuberculosis collected between 2012 and 2014; mainly MDR-TB (n=40; 95.2%) and rifampicin-sensitive TB (n=2; 4.8%). The rate of drug-resistant pulmonary tuberculosis was 63.8%, and the rate of MDR-TB accounted for 23.2%. The mutations in rpoB were predominantly in codons 531 (27.5%) and 523 (17.5%), with rare occurrences of S522A (2.5%) and A532P (2.5%). Noticeably, there was a substitution mutation in codon 532, and a mutant strain of tuberculosis at seven codons in the rpoB gene had been detected. In conclusion, this study provided drug-resistant characteristics in patients with recurrent pulmonary TB and mutations of MDR-TB in Can Tho, Vietnam. The results show a mutation in codon 532 of the rpoB gene and a mutant strain of tuberculosis at all seven codons. These are remarkable and promising results for further studies and clinical applications. https://pharmacy.mahidol.ac.th/journal/_files/2022-49-3_8.pdftuberculosismdr-tbrrdrdrug resistancemultidrug-resistant |
| spellingShingle | Loan Thi Duong Khang Tan Do Dung Nhan Tran Dung Ngoc Tran Phuc Huu Luu Thang Nguyen Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam Pharmaceutical Sciences Asia tuberculosis mdr-tb rrdr drug resistance multidrug-resistant |
| title | Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam |
| title_full | Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam |
| title_fullStr | Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam |
| title_full_unstemmed | Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam |
| title_short | Prevalence of drug-resistant recurrent tuberculosis and new multidrug-resistant tuberculosis mutations detection in Can Tho, Vietnam |
| title_sort | prevalence of drug resistant recurrent tuberculosis and new multidrug resistant tuberculosis mutations detection in can tho vietnam |
| topic | tuberculosis mdr-tb rrdr drug resistance multidrug-resistant |
| url | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-3_8.pdf |
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