Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie
Abstract The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four cal...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-023-01225-2 |
| _version_ | 1797557192974401536 |
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| author | Marina Betancor Belén Marín Alicia Otero Carlos Hedman Antonio Romero Tomás Barrio Eloisa Sevilla Jean-Yves Douet Alvina Huor Juan José Badiola Olivier Andréoletti Rosa Bolea |
| author_facet | Marina Betancor Belén Marín Alicia Otero Carlos Hedman Antonio Romero Tomás Barrio Eloisa Sevilla Jean-Yves Douet Alvina Huor Juan José Badiola Olivier Andréoletti Rosa Bolea |
| author_sort | Marina Betancor |
| collection | DOAJ |
| description | Abstract The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle. |
| first_indexed | 2024-03-10T17:13:53Z |
| format | Article |
| id | doaj.art-2a677dd837164793b30505a9a15f2807 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-10T17:13:53Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-2a677dd837164793b30505a9a15f28072023-11-20T10:33:00ZengBMCVeterinary Research1297-97162023-10-015411710.1186/s13567-023-01225-2Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapieMarina Betancor0Belén Marín1Alicia Otero2Carlos Hedman3Antonio Romero4Tomás Barrio5Eloisa Sevilla6Jean-Yves Douet7Alvina Huor8Juan José Badiola9Olivier Andréoletti10Rosa Bolea11Centro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaServicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de ZaragozaUMR INRAE ENVT 1225 Interactions Hôtes-Agents Pathogènes, École Nationale Vétérinaire de ToulouseCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaUMR INRAE ENVT 1225 Interactions Hôtes-Agents Pathogènes, École Nationale Vétérinaire de ToulouseUMR INRAE ENVT 1225 Interactions Hôtes-Agents Pathogènes, École Nationale Vétérinaire de ToulouseCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaUMR INRAE ENVT 1225 Interactions Hôtes-Agents Pathogènes, École Nationale Vétérinaire de ToulouseCentro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Instituto Agroalimentario de Aragón - IA2, Universidad de ZaragozaAbstract The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle.https://doi.org/10.1186/s13567-023-01225-2PrionPrPScatypical scrapiebovine spongiform encephalopathy |
| spellingShingle | Marina Betancor Belén Marín Alicia Otero Carlos Hedman Antonio Romero Tomás Barrio Eloisa Sevilla Jean-Yves Douet Alvina Huor Juan José Badiola Olivier Andréoletti Rosa Bolea Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie Veterinary Research Prion PrPSc atypical scrapie bovine spongiform encephalopathy |
| title | Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie |
| title_full | Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie |
| title_fullStr | Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie |
| title_full_unstemmed | Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie |
| title_short | Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie |
| title_sort | detection of classical bse prions in asymptomatic cows after inoculation with atypical nor98 scrapie |
| topic | Prion PrPSc atypical scrapie bovine spongiform encephalopathy |
| url | https://doi.org/10.1186/s13567-023-01225-2 |
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