Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark
Actinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. To obtain a fast diagnosis and identify A. schaalii, we developed a TaqMan real-time quantitative PCR. Routine urine samples were obtained from 177 hospitalized patients and 75 outpa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2010-01-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/16/1/09-0761_article |
| _version_ | 1818259678250926080 |
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| author | Steffen Bank Anders Jensen Thomas M. Hansen Karen M. Søby Jørgen Prag |
| author_facet | Steffen Bank Anders Jensen Thomas M. Hansen Karen M. Søby Jørgen Prag |
| author_sort | Steffen Bank |
| collection | DOAJ |
| description | Actinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. To obtain a fast diagnosis and identify A. schaalii, we developed a TaqMan real-time quantitative PCR. Routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in Viborg County, Denmark, in 2008–2009. The PCR detected A. schaalii in 22% of samples from patients >60 years of age. This assay showed that A. schaalii is more common than implied by routine cultivation. In 90% of PCR-positive urine samples, other common uropathogens were identified. This finding suggests that A. schaalii is a common, undetected, bacterial pathogen. Our results suggest that A. schaalii may be a more common pathogen than previously thought, especially in patients with unexplained chronic urinary tract infections, who are often treated with trimethoprim or ciprofloxacin, to which A. schaalii is resistant. |
| first_indexed | 2024-12-12T18:19:15Z |
| format | Article |
| id | doaj.art-2a6783babfd24cf8abd24e720cae6e4d |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-12T18:19:15Z |
| publishDate | 2010-01-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-2a6783babfd24cf8abd24e720cae6e4d2022-12-22T00:16:11ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592010-01-01161768010.3201/eid1601.090761Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, DenmarkSteffen BankAnders JensenThomas M. HansenKaren M. SøbyJørgen PragActinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. To obtain a fast diagnosis and identify A. schaalii, we developed a TaqMan real-time quantitative PCR. Routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in Viborg County, Denmark, in 2008–2009. The PCR detected A. schaalii in 22% of samples from patients >60 years of age. This assay showed that A. schaalii is more common than implied by routine cultivation. In 90% of PCR-positive urine samples, other common uropathogens were identified. This finding suggests that A. schaalii is a common, undetected, bacterial pathogen. Our results suggest that A. schaalii may be a more common pathogen than previously thought, especially in patients with unexplained chronic urinary tract infections, who are often treated with trimethoprim or ciprofloxacin, to which A. schaalii is resistant.https://wwwnc.cdc.gov/eid/article/16/1/09-0761_articleActinobaculum schaaliireal-time PCRuropathogenurinary tract infectionsbacteriaresearch |
| spellingShingle | Steffen Bank Anders Jensen Thomas M. Hansen Karen M. Søby Jørgen Prag Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark Emerging Infectious Diseases Actinobaculum schaalii real-time PCR uropathogen urinary tract infections bacteria research |
| title | Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark |
| title_full | Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark |
| title_fullStr | Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark |
| title_full_unstemmed | Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark |
| title_short | Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark |
| title_sort | actinobaculum schaalii a common uropathogen in elderly patients denmark |
| topic | Actinobaculum schaalii real-time PCR uropathogen urinary tract infections bacteria research |
| url | https://wwwnc.cdc.gov/eid/article/16/1/09-0761_article |
| work_keys_str_mv | AT steffenbank actinobaculumschaaliiacommonuropathogeninelderlypatientsdenmark AT andersjensen actinobaculumschaaliiacommonuropathogeninelderlypatientsdenmark AT thomasmhansen actinobaculumschaaliiacommonuropathogeninelderlypatientsdenmark AT karenmsøby actinobaculumschaaliiacommonuropathogeninelderlypatientsdenmark AT jørgenprag actinobaculumschaaliiacommonuropathogeninelderlypatientsdenmark |