Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle
Background The relationship between cyclic status of cattle ovaries on in vitro embryo development up to the blastocyst stage was investigated. Materials and Methods Cattle ovaries were collected immediately after slaughter and divided into three categories based on their cyclic status, which inclu...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2011-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45073_61871e7d12e70de652896747070b1eab.pdf |
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| author | Akbar Pirestani Sayyed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Fariba Moulavi Parvaneh Abedi Hamid Gourabi Abdolhossein Shahverdi Ahmad Vosough Taqi Dizaj |
| author_facet | Akbar Pirestani Sayyed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Fariba Moulavi Parvaneh Abedi Hamid Gourabi Abdolhossein Shahverdi Ahmad Vosough Taqi Dizaj |
| author_sort | Akbar Pirestani |
| collection | DOAJ |
| description | Background The relationship between cyclic status of cattle ovaries on in vitro embryo development up to the blastocyst stage was investigated. Materials and Methods Cattle ovaries were collected immediately after slaughter and divided into three categories based on their cyclic status, which included: 1. the presence of a large follicle (LF), 2. the presence of a corpus luteum (CL) and 3. ovaries without LF or CL (WLCF). Oocytes of these ovaries were obtained and used for in vitro maturation and fertilization. Presumptive zygotes were then cultured up to the blastocyst stage in synthetic oviductal fluid culture medium. Results There were no significant differences between cleavage rates of the three groups. The rate of embryos in the compact morula stage for the CL group was 48.2% which was significantly higher than the related rate of the LF group (36.6%), but non-significantly higher than that of the ST group (45.7%). The highest blastocyst rate belonged to the CL group (54.6%) which was significantly greater than the WLCF group (32.9%) and non-significantly higher than the LF group (52.4%). There was no significant difference in blastocyst rates in the CL and LF groups. Conclusion Preselection of oocyte donor ovaries containing a CL or LF can be used as a feasible and non- invasive criterion to obtain the most competent oocytes capable of development to the blastocyst stage. |
| first_indexed | 2024-12-23T06:52:32Z |
| format | Article |
| id | doaj.art-2a6e4c02f6af4e569bc505e5629c6935 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-23T06:52:32Z |
| publishDate | 2011-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-2a6e4c02f6af4e569bc505e5629c69352022-12-21T17:56:23ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782011-01-014417217545073Effect of Ovarian Cyclic Status on In Vitro Embryo Production in CattleAkbar Pirestani0Sayyed Morteza Hosseini1Mahdi Hajian2Mohsen Forouzanfar3Fariba Moulavi4Parvaneh Abedi5Hamid Gourabi6Abdolhossein Shahverdi7Ahmad Vosough Taqi Dizaj8Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Khorasgan Branch, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Animal Science, Marvdasht Branch, Islamic Azad University, Marvdasht, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran4Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran5Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran6Department of Reproductive Imaging, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;7Department of Andrology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranBackground The relationship between cyclic status of cattle ovaries on in vitro embryo development up to the blastocyst stage was investigated. Materials and Methods Cattle ovaries were collected immediately after slaughter and divided into three categories based on their cyclic status, which included: 1. the presence of a large follicle (LF), 2. the presence of a corpus luteum (CL) and 3. ovaries without LF or CL (WLCF). Oocytes of these ovaries were obtained and used for in vitro maturation and fertilization. Presumptive zygotes were then cultured up to the blastocyst stage in synthetic oviductal fluid culture medium. Results There were no significant differences between cleavage rates of the three groups. The rate of embryos in the compact morula stage for the CL group was 48.2% which was significantly higher than the related rate of the LF group (36.6%), but non-significantly higher than that of the ST group (45.7%). The highest blastocyst rate belonged to the CL group (54.6%) which was significantly greater than the WLCF group (32.9%) and non-significantly higher than the LF group (52.4%). There was no significant difference in blastocyst rates in the CL and LF groups. Conclusion Preselection of oocyte donor ovaries containing a CL or LF can be used as a feasible and non- invasive criterion to obtain the most competent oocytes capable of development to the blastocyst stage.http://www.ijfs.ir/article_45073_61871e7d12e70de652896747070b1eab.pdfembryoestrous cyclecattleovary |
| spellingShingle | Akbar Pirestani Sayyed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Fariba Moulavi Parvaneh Abedi Hamid Gourabi Abdolhossein Shahverdi Ahmad Vosough Taqi Dizaj Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle International Journal of Fertility and Sterility embryo estrous cycle cattle ovary |
| title | Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle |
| title_full | Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle |
| title_fullStr | Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle |
| title_full_unstemmed | Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle |
| title_short | Effect of Ovarian Cyclic Status on In Vitro Embryo Production in Cattle |
| title_sort | effect of ovarian cyclic status on in vitro embryo production in cattle |
| topic | embryo estrous cycle cattle ovary |
| url | http://www.ijfs.ir/article_45073_61871e7d12e70de652896747070b1eab.pdf |
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