General Strategies for RNA X-ray Crystallography
An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal con...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2111 |
| _version_ | 1827752507213873152 |
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| author | Ryland W. Jackson Claire M. Smathers Aaron R. Robart |
| author_facet | Ryland W. Jackson Claire M. Smathers Aaron R. Robart |
| author_sort | Ryland W. Jackson |
| collection | DOAJ |
| description | An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice. |
| first_indexed | 2024-03-11T07:17:24Z |
| format | Article |
| id | doaj.art-2a6efbfbb8674c78969d1bca652a415f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:17:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2a6efbfbb8674c78969d1bca652a415f2023-11-17T08:12:08ZengMDPI AGMolecules1420-30492023-02-01285211110.3390/molecules28052111General Strategies for RNA X-ray CrystallographyRyland W. Jackson0Claire M. Smathers1Aaron R. Robart2Department of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, WV 20506, USADepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, WV 20506, USADepartment of Biochemistry and Molecular Medicine, West Virginia University, Morgantown, WV 20506, USAAn extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice.https://www.mdpi.com/1420-3049/28/5/2111RNAribozymeX-ray crystallographystructurestructural biology |
| spellingShingle | Ryland W. Jackson Claire M. Smathers Aaron R. Robart General Strategies for RNA X-ray Crystallography Molecules RNA ribozyme X-ray crystallography structure structural biology |
| title | General Strategies for RNA X-ray Crystallography |
| title_full | General Strategies for RNA X-ray Crystallography |
| title_fullStr | General Strategies for RNA X-ray Crystallography |
| title_full_unstemmed | General Strategies for RNA X-ray Crystallography |
| title_short | General Strategies for RNA X-ray Crystallography |
| title_sort | general strategies for rna x ray crystallography |
| topic | RNA ribozyme X-ray crystallography structure structural biology |
| url | https://www.mdpi.com/1420-3049/28/5/2111 |
| work_keys_str_mv | AT rylandwjackson generalstrategiesforrnaxraycrystallography AT clairemsmathers generalstrategiesforrnaxraycrystallography AT aaronrrobart generalstrategiesforrnaxraycrystallography |