Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles
Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chines...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4345 |
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| author | Xiaolin Li Zhongkai Zhu Lei Ye Zongjing Kang Xiaoping Zhang Yue Huang Bo Zhang Yuanfeng Zou |
| author_facet | Xiaolin Li Zhongkai Zhu Lei Ye Zongjing Kang Xiaoping Zhang Yue Huang Bo Zhang Yuanfeng Zou |
| author_sort | Xiaolin Li |
| collection | DOAJ |
| description | Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained from <i>Tuber panzhihuanense</i> and referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified from <i>T. pseudoexcavatum</i> (TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done. |
| first_indexed | 2024-03-10T16:08:27Z |
| format | Article |
| id | doaj.art-2a743f640cd7432e98cdf817f1d16362 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:08:27Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2a743f640cd7432e98cdf817f1d163622023-11-20T14:40:26ZengMDPI AGMolecules1420-30492020-09-012518434510.3390/molecules25184345Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese TrufflesXiaolin Li0Zhongkai Zhu1Lei Ye2Zongjing Kang3Xiaoping Zhang4Yue Huang5Bo Zhang6Yuanfeng Zou7Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSoil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaTruffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained from <i>Tuber panzhihuanense</i> and referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified from <i>T. pseudoexcavatum</i> (TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done.https://www.mdpi.com/1420-3049/25/18/4345truffle<i>Tuber panzhihuanense</i><i>Tuber pseudoexcavatum</i>polysaccharidesstructure |
| spellingShingle | Xiaolin Li Zhongkai Zhu Lei Ye Zongjing Kang Xiaoping Zhang Yue Huang Bo Zhang Yuanfeng Zou Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles Molecules truffle <i>Tuber panzhihuanense</i> <i>Tuber pseudoexcavatum</i> polysaccharides structure |
| title | Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles |
| title_full | Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles |
| title_fullStr | Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles |
| title_full_unstemmed | Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles |
| title_short | Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles |
| title_sort | comparison of the partial structure and antioxidant activity of polysaccharides from two species of chinese truffles |
| topic | truffle <i>Tuber panzhihuanense</i> <i>Tuber pseudoexcavatum</i> polysaccharides structure |
| url | https://www.mdpi.com/1420-3049/25/18/4345 |
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