Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results
Abstract Background Cell behavior is the key to tissue regeneration. Given the fact that most of the cells used in tissue engineering are anchorage-dependent, their behavior including adhesion, growth, migration, matrix synthesis, and differentiation is related to the design of the scaffolds. Thus,...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2018-09-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40824-018-0136-8 |
| _version_ | 1797284004045520896 |
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| author | Ibrahim Fatih Cengiz Joaquim Miguel Oliveira Rui L. Reis |
| author_facet | Ibrahim Fatih Cengiz Joaquim Miguel Oliveira Rui L. Reis |
| author_sort | Ibrahim Fatih Cengiz |
| collection | DOAJ |
| description | Abstract Background Cell behavior is the key to tissue regeneration. Given the fact that most of the cells used in tissue engineering are anchorage-dependent, their behavior including adhesion, growth, migration, matrix synthesis, and differentiation is related to the design of the scaffolds. Thus, characterization of the scaffolds is highly required. Micro-computed tomography (micro-CT) provides a powerful platform to analyze, visualize, and explore any portion of interest in the scaffold in a 3D fashion without cutting or destroying it with the benefit of almost no sample preparation need. Main body This review highlights the relationship between the scaffold microstructure and cell behavior, and provides the basics of the micro-CT method. In this work, we also analyzed the original papers that were published in 2016 through a systematic search to address the need for specific improvements in the methods section of the papers including the amount of provided information from the obtained results. Conclusion Micro-CT offers a unique microstructural analysis of biomaterials, notwithstanding the associated challenges and limitations. Future studies that will include micro-CT characterization of scaffolds should report the important details of the method, and the derived quantitative and qualitative information can be maximized. |
| first_indexed | 2024-03-07T17:42:41Z |
| format | Article |
| id | doaj.art-2a8079ae05024780b01222f526c34305 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T17:42:41Z |
| publishDate | 2018-09-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-2a8079ae05024780b01222f526c343052024-03-02T15:54:51ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242018-09-0122111110.1186/s40824-018-0136-8Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and resultsIbrahim Fatih Cengiz0Joaquim Miguel Oliveira1Rui L. Reis23B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAbstract Background Cell behavior is the key to tissue regeneration. Given the fact that most of the cells used in tissue engineering are anchorage-dependent, their behavior including adhesion, growth, migration, matrix synthesis, and differentiation is related to the design of the scaffolds. Thus, characterization of the scaffolds is highly required. Micro-computed tomography (micro-CT) provides a powerful platform to analyze, visualize, and explore any portion of interest in the scaffold in a 3D fashion without cutting or destroying it with the benefit of almost no sample preparation need. Main body This review highlights the relationship between the scaffold microstructure and cell behavior, and provides the basics of the micro-CT method. In this work, we also analyzed the original papers that were published in 2016 through a systematic search to address the need for specific improvements in the methods section of the papers including the amount of provided information from the obtained results. Conclusion Micro-CT offers a unique microstructural analysis of biomaterials, notwithstanding the associated challenges and limitations. Future studies that will include micro-CT characterization of scaffolds should report the important details of the method, and the derived quantitative and qualitative information can be maximized.http://link.springer.com/article/10.1186/s40824-018-0136-8Systematic reviewMicrostructureMineral densityScaffoldsTissue engineering |
| spellingShingle | Ibrahim Fatih Cengiz Joaquim Miguel Oliveira Rui L. Reis Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results Biomaterials Research Systematic review Microstructure Mineral density Scaffolds Tissue engineering |
| title | Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results |
| title_full | Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results |
| title_fullStr | Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results |
| title_full_unstemmed | Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results |
| title_short | Micro-CT – a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results |
| title_sort | micro ct a digital 3d microstructural voyage into scaffolds a systematic review of the reported methods and results |
| topic | Systematic review Microstructure Mineral density Scaffolds Tissue engineering |
| url | http://link.springer.com/article/10.1186/s40824-018-0136-8 |
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