A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms

A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms

Pathogenic bacteria form biofilms during infection, and polymicrobial biofilms are the most frequent manifestation. Biofilm attachment, maturation, and/or antibiotic sensitivity are mainly evaluated with microtiter plate assays, in which bacteria are stained to enable the quantification of the bioma...

Full description

Bibliographic Details
Main Authors:	Víctor Campo-Pérez, Júlia Alcàcer-Almansa, Esther Julián, Eduard Torrents
Format:	Article
Language:	English
Published:	MDPI AG 2023-09-01
Series:	Microorganisms
Subjects:	crystal violet biomass quantification <i>Pseudomonas aeruginosa</i> <i>Burkholderia cenocepacia</i> dual-species biofilms
Online Access:	https://www.mdpi.com/2076-2607/11/9/2244

Similar Items

A Polyclonal Antibody against a <i>Burkholderia cenocepacia</i> OmpA-like Protein Strongly Impairs <i>Pseudomonas aeruginosa</i> and <i>B. multivorans</i> Virulence
by: António M. M. Seixas, et al.
Published: (2024-02-01)

Relationship between Biofilm-Formation, Phenotypic Virulence Factors and Antibiotic Resistance in Environmental <i>Pseudomonas</i> <i>aeruginosa</i>
by: Payam Behzadi, et al.
Published: (2022-09-01)

Into the well—A close look at the complex structures of a microtiter biofilm and the crystal violet assay
by: Kasper Nørskov Kragh, et al.
Published: (2019-12-01)

Bacterial Subversion of Autophagy in Cystic Fibrosis
by: Verónica Roxana Flores-Vega, et al.
Published: (2021-10-01)

Revealing the Metabolic Alterations during Biofilm Development of <i>Burkholderia cenocepacia</i> Based on Genome-Scale Metabolic Modeling
by: Ozlem Altay, et al.
Published: (2021-04-01)

Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains
by: Tomasz Bogiel, et al.
Published: (2021-02-01)

Fibrosis quística: patogenia bacteriana y moduladores del CFTR (regulador de conductancia transmembranal de la fibrosis quística)
by: Silvia Y. Vargas-Roldán, et al.
Published: (2022-10-01)

<i>Pseudomonas aeruginosa</i> Biofilms
by: Minh Tam Tran Thi, et al.
Published: (2020-11-01)

A Cationic Porphyrin, ZnPor, Disassembles <i>Pseudomonas aeruginosa</i> Biofilm Matrix, Kills Cells Directly, and Enhances Antibiotic Activity of Tobramycin
by: Neha Patel, et al.
Published: (2020-12-01)

Pitfalls Associated with Discriminating Mixed-Species Biofilms by Flow Cytometry
by: Tânia Grainha, et al.
Published: (2020-10-01)

The Biofilm Inhibition Properties of Glucosamine Gold Nanoparticles in Combination with Meropenem against <i>Pseudomonas aeruginosa</i> on the Endotracheal Tube: A Model of Biofilm-Related Ventilator-Associated Pneumonia
by: Dewi Santosaningsih, et al.
Published: (2024-03-01)

Deferiprone-Gallium-Protoporphyrin Chitogel Decreases <i>Pseudomonas aeruginosa</i> Biofilm Infection without Impairing Wound Healing
by: Tahlia L. Kennewell, et al.
Published: (2024-02-01)

Dual-Species Biofilms: Biomass, Viable Cell Ratio/Cross-Species Interactions, Conjugative Transfer
by: Marina V. Kuznetsova, et al.
Published: (2023-09-01)

Pathogenesis of the <i>Pseudomonas aeruginosa</i> Biofilm: A Review
by: Felipe Francisco Tuon, et al.
Published: (2022-02-01)

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate
by: Mohammad Abdulraheem Al-kafaween, et al.
Published: (2019-06-01)

Multicenter surveillance of antimicrobial susceptibilities and resistance mechanisms among Enterobacterales species and non-fermenting Gram-negative bacteria from different infection sources in Taiwan from 2016 to 2018
by: Shio-Shin Jean, et al.
Published: (2022-06-01)

Unveiling the Anti-Biofilm Property of Hydroxyapatite on <i>Pseudomonas aeruginosa</i>: Synthesis and Strategy
by: Davoodbasha MubarakAli, et al.
Published: (2023-01-01)

Bacteriophage Cocktail-Mediated Inhibition of <i>Pseudomonas aeruginosa</i> Biofilm on Endotracheal Tube Surface
by: Viviane C. Oliveira, et al.
Published: (2021-01-01)

The Small RNA ncS35 Regulates Growth in <named-content content-type="genus-species">Burkholderia cenocepacia</named-content> J2315
by: Sanne Kiekens, et al.
Published: (2018-02-01)

Immune Response to Biofilm Growing Pulmonary <i>Pseudomonas aeruginosa</i> Infection
by: Kim Thomsen, et al.
Published: (2022-08-01)

Liposomes-Based Drug Delivery Systems of Anti-Biofilm Agents to Combat Bacterial Biofilm Formation
by: Zinb Makhlouf, et al.
Published: (2023-05-01)

Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms
by: Ergun Akturk, et al.
Published: (2019-07-01)

Virulence Genes as Markers for <i>Pseudomonas aeruginosa</i> Biofilm Formation in Dogs and Cats
by: Daria Płókarz, et al.
Published: (2022-02-01)

Inhibition of <i>Pseudomonas aeruginosa</i> Biofilm Formation with Surface Modified Polymeric Nanoparticles
by: Tyler R. Flockton, et al.
Published: (2019-04-01)

Marine Bacteria, A Source for Alginolytic Enzyme to Disrupt <i>Pseudomonas aeruginosa</i> Biofilms
by: Said M. Daboor, et al.
Published: (2019-05-01)

Tracking of Bacteriophage Predation on <i>Pseudomonas aeruginosa</i> Using a New Radiofrequency Biofilm Sensor
by: Matthieu Longo, et al.
Published: (2024-03-01)

A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of <i>Pseudomonas fluorescens</i> and <i>Staphylococcus epidermidis</i>
by: Valeria Angarano, et al.
Published: (2020-06-01)

Design, Synthesis, and Biological Evaluation of Phenyloxadiazole Sulfoxide Derivatives as Potent <i>Pseudomonas aeruginosa</i> Biofilm Inhibitors
by: Xinyi Ye, et al.
Published: (2023-05-01)

Study of Antimicrobial Resistance, Biofilm Formation, and Motility of <i>Pseudomonas aeruginosa</i> Derived from Urine Samples
by: Telma de Sousa, et al.
Published: (2023-05-01)

Characterization of Volatile Organic Compounds Emitted from Endophytic <i>Burkholderia cenocepacia</i> ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens
by: Jian-Hua Chen, et al.
Published: (2020-08-01)

Dissolution of Biofilm Secreted by Three Different Strains of <i>Pseudomonas aeruginosa</i> with Bromelain, <i>N</i>-Acetylcysteine, and Their Combinations
by: Carly J. Carter, et al.
Published: (2021-12-01)

Pharmacological Characters and Toxicity Evaluation of Coumarin Derivative LP4C as Lead Compound against Biofilm Formation of <i>Pseudomonas aeruginosa</i>
by: Moxi Yu, et al.
Published: (2023-03-01)

Recent Advance in Small Molecules Targeting RhlR of <i>Pseudomonas</i> <i>aeruginosa</i>
by: Taehyeong Lim, et al.
Published: (2022-02-01)

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in <i>Pseudomonas aeruginosa</i>: Results from a Laboratory-Based In Vitro Study
by: Márió Gajdács, et al.
Published: (2021-09-01)

Pepsin and Trypsin Treatment Combined with Carvacrol: An Efficient Strategy to Fight <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i> Biofilms
by: Samah Mechmechani, et al.
Published: (2023-01-01)

Effects of аntimicrobials on <i>Pseudomonas aeruginosa</i> biofilm formation
by: U. M. Nemchenko, et al.
Published: (2022-09-01)

Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy against <i>Pseudomonas aeruginosa</i>
by: Emilie Charpentier, et al.
Published: (2021-10-01)

Impact of <i>Solidago virgaurea</i> Extract on Biofilm Formation for ESBL-<i>Pseudomonas aeruginosa</i>: An <i>In Vitro</i> Model Study
by: Ali Hazim Abdulkareem, et al.
Published: (2023-09-01)

Targeting Persistent Biofilm Infections: Reconsidering the Topography of the Infection Site during Model Selection
by: Ilana Kolodkin-Gal, et al.
Published: (2022-06-01)

Itaconic Acid Increases the Efficacy of Tobramycin against <i>Pseudomonas aeruginosa</i> Biofilms
by: Duy-Khiet Ho, et al.
Published: (2020-07-01)