Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease.
<h4>Background</h4>Inflammatory bowel diseases (IBDs) with the subtypes ulcerative colitis (UC) and Crohn disease (CD), are chronic autoimmune inflammatory disorders of the gastrointestinal tract. Cytokines are associated with the development and progression in pediatric IBD. We measured...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0288147&type=printable |
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| author | Andrea Ott Erol Tutdibi Sybelle Goedicke-Fritz Jakob Schöpe Michael Zemlin Nasenien Nourkami-Tutdibi |
| author_facet | Andrea Ott Erol Tutdibi Sybelle Goedicke-Fritz Jakob Schöpe Michael Zemlin Nasenien Nourkami-Tutdibi |
| author_sort | Andrea Ott |
| collection | DOAJ |
| description | <h4>Background</h4>Inflammatory bowel diseases (IBDs) with the subtypes ulcerative colitis (UC) and Crohn disease (CD), are chronic autoimmune inflammatory disorders of the gastrointestinal tract. Cytokines are associated with the development and progression in pediatric IBD. We measured cytokine levels in pediatric IBD patients to assess their potential function as biomarkers in disease assessment.<h4>Method</h4>In this prospective cohort study, we enrolled 33 children with IBD. All patients were in stable remission for 3 months on enrollment. Patients who developed a relapse within six months after enrollment were classified as relapsers. Blood sampling was performed at enrolment and for relapsers in relapse and post-relapse. Serum concentrations of 14 cytokines, chemokines and growth factors (IL-1α, IL-1β, IL-6, IL-12p40, IP-10, TNF-α, IFN-γ, IL-10, IL-8, MIP-1α, MCP-1, MCP-3, G-CSF, GM-CSF) were measured simultaneously using multiplex bead-based sandwich immunoassay on Luminex 100 system.<h4>Results</h4>MCP-1 was significantly higher in CD patients compared to UC patients at each disease stage: stable remission (P<0.048), unstable remission (P<0.013), relapse (P<0.026) and post-relapse (P<0.024). G-CSF was significantly increased in UC patients developing a relapse and in post-relapse stage compared to UC patients in remission (P<0.02 and p<0.03, respectively).<h4>Conclusion</h4>MCP-1 showed potential as a diagnostic biomarker in CD patients independent of disease activity as it was able to discriminate between subtypes of pediatric IBD. In UC patients, G-CSF was significantly elevated in relapsers indicating its use and role as a potential prognostic biomarker. |
| first_indexed | 2024-03-11T11:40:16Z |
| format | Article |
| id | doaj.art-2a8102fc7a6a4ae0a718069af37c5f91 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T11:40:16Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2a8102fc7a6a4ae0a718069af37c5f912023-11-10T05:31:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011811e028814710.1371/journal.pone.0288147Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease.Andrea OttErol TutdibiSybelle Goedicke-FritzJakob SchöpeMichael ZemlinNasenien Nourkami-Tutdibi<h4>Background</h4>Inflammatory bowel diseases (IBDs) with the subtypes ulcerative colitis (UC) and Crohn disease (CD), are chronic autoimmune inflammatory disorders of the gastrointestinal tract. Cytokines are associated with the development and progression in pediatric IBD. We measured cytokine levels in pediatric IBD patients to assess their potential function as biomarkers in disease assessment.<h4>Method</h4>In this prospective cohort study, we enrolled 33 children with IBD. All patients were in stable remission for 3 months on enrollment. Patients who developed a relapse within six months after enrollment were classified as relapsers. Blood sampling was performed at enrolment and for relapsers in relapse and post-relapse. Serum concentrations of 14 cytokines, chemokines and growth factors (IL-1α, IL-1β, IL-6, IL-12p40, IP-10, TNF-α, IFN-γ, IL-10, IL-8, MIP-1α, MCP-1, MCP-3, G-CSF, GM-CSF) were measured simultaneously using multiplex bead-based sandwich immunoassay on Luminex 100 system.<h4>Results</h4>MCP-1 was significantly higher in CD patients compared to UC patients at each disease stage: stable remission (P<0.048), unstable remission (P<0.013), relapse (P<0.026) and post-relapse (P<0.024). G-CSF was significantly increased in UC patients developing a relapse and in post-relapse stage compared to UC patients in remission (P<0.02 and p<0.03, respectively).<h4>Conclusion</h4>MCP-1 showed potential as a diagnostic biomarker in CD patients independent of disease activity as it was able to discriminate between subtypes of pediatric IBD. In UC patients, G-CSF was significantly elevated in relapsers indicating its use and role as a potential prognostic biomarker.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0288147&type=printable |
| spellingShingle | Andrea Ott Erol Tutdibi Sybelle Goedicke-Fritz Jakob Schöpe Michael Zemlin Nasenien Nourkami-Tutdibi Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. PLoS ONE |
| title | Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. |
| title_full | Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. |
| title_fullStr | Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. |
| title_full_unstemmed | Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. |
| title_short | Serum cytokines MCP-1 and GCS-F as potential biomarkers in pediatric inflammatory bowel disease. |
| title_sort | serum cytokines mcp 1 and gcs f as potential biomarkers in pediatric inflammatory bowel disease |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0288147&type=printable |
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