Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5]
Objective: To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women. Methods: SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Portuguese |
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Brazilian Society of Pediatrics
2013-09-01
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| Series: | Jornal de Pediatria (Versão em Português) |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2255553613000785 |
| _version_ | 1811323801107955712 |
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| author | Simone M.R. Santos Thalita L. Ferreira Virgínia S. Quintal Solange B. Carbonare Milene Tino-De-Franco |
| author_facet | Simone M.R. Santos Thalita L. Ferreira Virgínia S. Quintal Solange B. Carbonare Milene Tino-De-Franco |
| author_sort | Simone M.R. Santos |
| collection | DOAJ |
| description | Objective: To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women.
Methods: SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples’ capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay.
Results: Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers.
Conclusions: The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice. |
| first_indexed | 2024-04-13T14:01:54Z |
| format | Article |
| id | doaj.art-2a85895b91fe479b81e0ea662fe49beb |
| institution | Directory Open Access Journal |
| issn | 2255-5536 |
| language | Portuguese |
| last_indexed | 2024-04-13T14:01:54Z |
| publishDate | 2013-09-01 |
| publisher | Brazilian Society of Pediatrics |
| record_format | Article |
| series | Jornal de Pediatria (Versão em Português) |
| spelling | doaj.art-2a85895b91fe479b81e0ea662fe49beb2022-12-22T02:44:01ZporBrazilian Society of PediatricsJornal de Pediatria (Versão em Português)2255-55362013-09-0189551051310.1016/j.jpedp.2012.12.010Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5]Simone M.R. Santos0Thalita L. Ferreira1Virgínia S. Quintal2Solange B. Carbonare3Milene Tino-De-Franco4Mestre, Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, BrasilMestre, Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, BrasilMédica, Banco de Leite Humano, Hospital Universitário da Universidade de São Paulo, São Paulo, SP, BrasilDoutora, Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, BrasilDoutora, Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, BrasilObjective: To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women. Methods: SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples’ capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay. Results: Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers. Conclusions: The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice.http://www.sciencedirect.com/science/article/pii/S2255553613000785Human milkRotavirusSecretory IgARotavirus neutralization |
| spellingShingle | Simone M.R. Santos Thalita L. Ferreira Virgínia S. Quintal Solange B. Carbonare Milene Tino-De-Franco Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] Jornal de Pediatria (Versão em Português) Human milk Rotavirus Secretory IgA Rotavirus neutralization |
| title | Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] |
| title_full | Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] |
| title_fullStr | Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] |
| title_full_unstemmed | Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] |
| title_short | Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5] |
| title_sort | milk from brazilian women presents secretory iga antibodies and neutralizes rotavirus g9p 5 |
| topic | Human milk Rotavirus Secretory IgA Rotavirus neutralization |
| url | http://www.sciencedirect.com/science/article/pii/S2255553613000785 |
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