Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials
Abstract The burgeoning cellulosic ethanol industry necessitates advancements in enzymatic saccharification, effective pretreatments for lignin removal, and the cultivation of crops more amenable to saccharification. Studies have demonstrated that natural inhibitors of lignin biosynthesis can enhanc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | GCB Bioenergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/gcbb.13108 |
| _version_ | 1797628829539237888 |
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| author | Viviane Fátima deOliveira Lucas Figueiredo Gabriel deOliveira Correia Maria de Fátima Pires daSilva Machado Hugo Zeni Neto Wanderley Dantas dosSantos Claudete Aparecida Mangolin |
| author_facet | Viviane Fátima deOliveira Lucas Figueiredo Gabriel deOliveira Correia Maria de Fátima Pires daSilva Machado Hugo Zeni Neto Wanderley Dantas dosSantos Claudete Aparecida Mangolin |
| author_sort | Viviane Fátima deOliveira |
| collection | DOAJ |
| description | Abstract The burgeoning cellulosic ethanol industry necessitates advancements in enzymatic saccharification, effective pretreatments for lignin removal, and the cultivation of crops more amenable to saccharification. Studies have demonstrated that natural inhibitors of lignin biosynthesis can enhance the saccharification of lignocellulose, even in tissues generated several months post‐treatment. In this study, we applied daidzin (a competitive inhibitor of coniferaldehyde dehydrogenase), piperonylic acid (a quasi‐irreversible inhibitor of cinnamate 4‐hydroxylase), and methylenedioxy cinnamic acid (a competitive inhibitor of 4‐coenzyme A ligase) to 60‐day‐old crops of two conventional Brazilian sugarcane cultivars and two energy cane clones, bred specifically for enhanced biomass production. The resultant biomasses were evaluated for lignin content and enzymatic saccharification efficiency without additional lignin‐removal pretreatments. The treatments amplified the production of fermentable sugars in both the sugarcane cultivars and energy cane clones. The most successful results softened the most recalcitrant lignocellulose to the level of the least recalcitrant of the biomasses tested. Interestingly, the softest material became even more susceptible to saccharification. |
| first_indexed | 2024-03-11T10:45:43Z |
| format | Article |
| id | doaj.art-2a85aff0f1f2490a81964f26d9ec166e |
| institution | Directory Open Access Journal |
| issn | 1757-1693 1757-1707 |
| language | English |
| last_indexed | 2024-03-11T10:45:43Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj.art-2a85aff0f1f2490a81964f26d9ec166e2023-11-14T05:58:57ZengWileyGCB Bioenergy1757-16931757-17072023-12-0115121465147610.1111/gcbb.13108Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trialsViviane Fátima deOliveira0Lucas Figueiredo1Gabriel deOliveira Correia2Maria de Fátima Pires daSilva Machado3Hugo Zeni Neto4Wanderley Dantas dosSantos5Claudete Aparecida Mangolin6Graduate Student in Environmental Biotechnology State University of Maringá Maringá PR BrazilGraduate Student in Cell Biology State University of Maringá Maringá PR BrazilGraduation Student in Biotechnology State University of Maringá Maringá PR BrazilDepartment of Biotechnology, Genetics and Cell Biology State University of Maringá Maringá PR BrazilDepartment of Agronomy State University of Maringá Maringá PR BrazilDepartment of Biochemistry State University of Maringá Maringá PR BrazilDepartment of Biotechnology, Genetics and Cell Biology State University of Maringá Maringá PR BrazilAbstract The burgeoning cellulosic ethanol industry necessitates advancements in enzymatic saccharification, effective pretreatments for lignin removal, and the cultivation of crops more amenable to saccharification. Studies have demonstrated that natural inhibitors of lignin biosynthesis can enhance the saccharification of lignocellulose, even in tissues generated several months post‐treatment. In this study, we applied daidzin (a competitive inhibitor of coniferaldehyde dehydrogenase), piperonylic acid (a quasi‐irreversible inhibitor of cinnamate 4‐hydroxylase), and methylenedioxy cinnamic acid (a competitive inhibitor of 4‐coenzyme A ligase) to 60‐day‐old crops of two conventional Brazilian sugarcane cultivars and two energy cane clones, bred specifically for enhanced biomass production. The resultant biomasses were evaluated for lignin content and enzymatic saccharification efficiency without additional lignin‐removal pretreatments. The treatments amplified the production of fermentable sugars in both the sugarcane cultivars and energy cane clones. The most successful results softened the most recalcitrant lignocellulose to the level of the least recalcitrant of the biomasses tested. Interestingly, the softest material became even more susceptible to saccharification.https://doi.org/10.1111/gcbb.13108biofuelsenergy caneenzyme inhibitors saccharificationlignocelluloseSaccharum spp |
| spellingShingle | Viviane Fátima deOliveira Lucas Figueiredo Gabriel deOliveira Correia Maria de Fátima Pires daSilva Machado Hugo Zeni Neto Wanderley Dantas dosSantos Claudete Aparecida Mangolin Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials GCB Bioenergy biofuels energy cane enzyme inhibitors saccharification lignocellulose Saccharum spp |
| title | Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| title_full | Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| title_fullStr | Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| title_full_unstemmed | Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| title_short | Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| title_sort | natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials |
| topic | biofuels energy cane enzyme inhibitors saccharification lignocellulose Saccharum spp |
| url | https://doi.org/10.1111/gcbb.13108 |
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