Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition
Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages incl...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga, Faculty of Veterinary Medicine
2024-04-01
|
| Series: | Jurnal Medik Veteriner |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/51740 |
| _version_ | 1797232506076921856 |
|---|---|
| author | Diena Delaiah Ahmad Aswin Helen Susilowati Andi Yasmin Wijaya Firdausy Kurnia Maulana Diyantoro Diyantoro Watchareewan Rodprasert Yulianna Puspitasari Yeni Dhamayanti Suryo Kuncorojakti |
| author_facet | Diena Delaiah Ahmad Aswin Helen Susilowati Andi Yasmin Wijaya Firdausy Kurnia Maulana Diyantoro Diyantoro Watchareewan Rodprasert Yulianna Puspitasari Yeni Dhamayanti Suryo Kuncorojakti |
| author_sort | Diena Delaiah |
| collection | DOAJ |
| description | Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells. |
| first_indexed | 2024-04-24T16:01:21Z |
| format | Article |
| id | doaj.art-2a86d6d8295b4b018cc1a821c7c706d8 |
| institution | Directory Open Access Journal |
| issn | 2615-7497 2581-012X |
| language | English |
| last_indexed | 2024-04-24T16:01:21Z |
| publishDate | 2024-04-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj.art-2a86d6d8295b4b018cc1a821c7c706d82024-04-01T07:00:11ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-04-01711610.20473/jmv.vol7.iss1.2024.1-649899Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free ConditionDiena Delaiah0https://orcid.org/0009-0001-1931-5796Ahmad Aswin1Helen Susilowati2https://orcid.org/0000-0002-3358-5231Andi Yasmin Wijaya3https://orcid.org/0000-0002-6463-6265Firdausy Kurnia Maulana4https://orcid.org/0000-0002-8276-6151Diyantoro Diyantoro5https://orcid.org/0000-0003-0175-8000Watchareewan Rodprasert6https://orcid.org/0000-0002-6134-1461Yulianna Puspitasari7https://orcid.org/0000-0002-1198-8515Yeni Dhamayanti8https://orcid.org/0000-0002-0353-2489Suryo Kuncorojakti9https://orcid.org/0000-0001-5170-9982Research Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaResearch Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaResearch Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaResearch Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaResearch Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaFaculty of Vocational Studies, Universitas Airlangga, Surabaya, IndonesiaVeterinary Stem Cell and Bioengineering Innovation Center, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDivision of Veterinary Microbiology, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDivision of Veterinary Anatomy, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDivision of Veterinary Anatomy, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaVero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.https://e-journal.unair.ac.id/JMV/article/view/51740cell culturecell-based vaccine productionconditioned mediavaccine |
| spellingShingle | Diena Delaiah Ahmad Aswin Helen Susilowati Andi Yasmin Wijaya Firdausy Kurnia Maulana Diyantoro Diyantoro Watchareewan Rodprasert Yulianna Puspitasari Yeni Dhamayanti Suryo Kuncorojakti Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition Jurnal Medik Veteriner cell culture cell-based vaccine production conditioned media vaccine |
| title | Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition |
| title_full | Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition |
| title_fullStr | Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition |
| title_full_unstemmed | Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition |
| title_short | Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition |
| title_sort | epidermal growth factor promotes e6 and ccl 81 vero cells proliferation under serum free condition |
| topic | cell culture cell-based vaccine production conditioned media vaccine |
| url | https://e-journal.unair.ac.id/JMV/article/view/51740 |
| work_keys_str_mv | AT dienadelaiah epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT ahmadaswin epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT helensusilowati epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT andiyasminwijaya epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT firdausykurniamaulana epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT diyantorodiyantoro epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT watchareewanrodprasert epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT yuliannapuspitasari epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT yenidhamayanti epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition AT suryokuncorojakti epidermalgrowthfactorpromotese6andccl81verocellsproliferationunderserumfreecondition |