Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats
Objective: Yam or diosgenin (extracted from the root of wild yam) is traditionally used for hormone replacement in menopausal women. Calpains are crucially related to the degradation of myofibrillar proteins in skeletal muscle. This study aimed to investigate the effects of yam and diosgenin on the...
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| Format: | Article |
| Language: | English |
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Elsevier
2008-06-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455908600777 |
| _version_ | 1819230591581683712 |
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| author | Kung-Hao Hsu Chi-Chen Chang Horng-Der Tsai Fuu-Jen Tsai Yao-Yuan Hsieh |
| author_facet | Kung-Hao Hsu Chi-Chen Chang Horng-Der Tsai Fuu-Jen Tsai Yao-Yuan Hsieh |
| author_sort | Kung-Hao Hsu |
| collection | DOAJ |
| description | Objective: Yam or diosgenin (extracted from the root of wild yam) is traditionally used for hormone replacement in menopausal women. Calpains are crucially related to the degradation of myofibrillar proteins in skeletal muscle. This study aimed to investigate the effects of yam and diosgenin on the calpain isoform expression in ovariectomized rats, a model of menopausal status.
Materials and Methods: Female rats were divided into: (1) controls; (2) ovariectomized rats; (3) ovariectomized rats receiving yam (250, 750, 1,500 mg/kg/day); (4) ovariectomized rats receiving diosgenin (10, 50, 100 mg/kg/day). Yam and diosgenin were administered for 8 weeks. The expression of μ- and m-calpain in skeletal muscles was determined by reverse transcriptase-polymerase chain reaction.
Results: The μ-calpain/β-actin and m-calpain/β-actin ratios in the control group (0.9 and 1.09, respectively) were significantly higher than those in the ovariectomized group (0.58 and 0.72, respectively). In the yam group, the expression of μ- and m-calpain was lowest in the ovariectomized group receiving no supplementation and lower in the 250 mg group compared with the 750 and 1,500 mg groups (for 0, 250, 750 and 1,500 mg dosage groups, μ-calpain, 0.58, 0.88, 1.24 and 1.13, respectively; m-calpain, 0.72, 1.02, 1.38 and 1.47, respectively). In contrast, there were no significant differences in the expression of μ- and m-calpain mRNAs among the different diosgenin dosage groups (for 0, 1 0, 50 and 100 mg of diosgenin, μ-calpain, 0.58, 0.56, 0.62 and 0.58, respectively; m-calpain, 0.72, 0.58, 0.71 and 0.54, respectively). Decreased expression of μ- or m-calpain was observed in the ovariectomized group compared with the normal controls.
Conclusion: Yam, but not its extract (diosgenin), is associated with the regulation of calpain isoforms in ovariectomized rats. Adequate yam supplements might improve the muscular calpain-related physiopathology associated with menopausal status. [Taiwan J Obstet Cynecol 2008;47(2):1 80-186] |
| first_indexed | 2024-12-23T11:31:30Z |
| format | Article |
| id | doaj.art-2a87892dbacc44c6b36051c6298405b7 |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-23T11:31:30Z |
| publishDate | 2008-06-01 |
| publisher | Elsevier |
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| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-2a87892dbacc44c6b36051c6298405b72022-12-21T17:48:48ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592008-06-0147218018610.1016/S1028-4559(08)60077-7Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized RatsKung-Hao Hsu0Chi-Chen Chang1Horng-Der Tsai2Fuu-Jen Tsai3Yao-Yuan Hsieh4Department of Animal Science and Biotechnology, Tunghai University, Taichung, TaiwanDepartment of Obstetrics and Gynecology, China Medical University, Taichung, TaiwanDepartment of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taichung, TaiwanDepartment of Pediatrics and genetics, China Medical University, Taichung, TaiwanDepartment of Obstetrics and Gynecology, China Medical University, Taichung, TaiwanObjective: Yam or diosgenin (extracted from the root of wild yam) is traditionally used for hormone replacement in menopausal women. Calpains are crucially related to the degradation of myofibrillar proteins in skeletal muscle. This study aimed to investigate the effects of yam and diosgenin on the calpain isoform expression in ovariectomized rats, a model of menopausal status. Materials and Methods: Female rats were divided into: (1) controls; (2) ovariectomized rats; (3) ovariectomized rats receiving yam (250, 750, 1,500 mg/kg/day); (4) ovariectomized rats receiving diosgenin (10, 50, 100 mg/kg/day). Yam and diosgenin were administered for 8 weeks. The expression of μ- and m-calpain in skeletal muscles was determined by reverse transcriptase-polymerase chain reaction. Results: The μ-calpain/β-actin and m-calpain/β-actin ratios in the control group (0.9 and 1.09, respectively) were significantly higher than those in the ovariectomized group (0.58 and 0.72, respectively). In the yam group, the expression of μ- and m-calpain was lowest in the ovariectomized group receiving no supplementation and lower in the 250 mg group compared with the 750 and 1,500 mg groups (for 0, 250, 750 and 1,500 mg dosage groups, μ-calpain, 0.58, 0.88, 1.24 and 1.13, respectively; m-calpain, 0.72, 1.02, 1.38 and 1.47, respectively). In contrast, there were no significant differences in the expression of μ- and m-calpain mRNAs among the different diosgenin dosage groups (for 0, 1 0, 50 and 100 mg of diosgenin, μ-calpain, 0.58, 0.56, 0.62 and 0.58, respectively; m-calpain, 0.72, 0.58, 0.71 and 0.54, respectively). Decreased expression of μ- or m-calpain was observed in the ovariectomized group compared with the normal controls. Conclusion: Yam, but not its extract (diosgenin), is associated with the regulation of calpain isoforms in ovariectomized rats. Adequate yam supplements might improve the muscular calpain-related physiopathology associated with menopausal status. [Taiwan J Obstet Cynecol 2008;47(2):1 80-186]http://www.sciencedirect.com/science/article/pii/S1028455908600777calpaindiosgeninmenopauseovariectomyyam |
| spellingShingle | Kung-Hao Hsu Chi-Chen Chang Horng-Der Tsai Fuu-Jen Tsai Yao-Yuan Hsieh Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats Taiwanese Journal of Obstetrics & Gynecology calpain diosgenin menopause ovariectomy yam |
| title | Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats |
| title_full | Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats |
| title_fullStr | Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats |
| title_full_unstemmed | Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats |
| title_short | Effects of Yam and Diosgenin on Calpain Systems in Skeletal Muscle of Ovariectomized Rats |
| title_sort | effects of yam and diosgenin on calpain systems in skeletal muscle of ovariectomized rats |
| topic | calpain diosgenin menopause ovariectomy yam |
| url | http://www.sciencedirect.com/science/article/pii/S1028455908600777 |
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