The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging
A decrease in the regenerative potential of the liver during the development of non-alcoholic fatty liver disease (NAFLD), which is observed in the vast majority of patients with diabetes mellitus type 1, significantly increases the risk of postoperative liver failure. In this regard, it is necessar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/17016 |
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| author | Svetlana Rodimova Nikolai Bobrov Artem Mozherov Vadim Elagin Maria Karabut Polina Ermakova Ilya Shchechkin Dmitry Kozlov Dmitry Krylov Alena Gavrina Aleksandra Kashina Vladimir Zagainov Elena Zagaynova Daria Kuznetsova |
| author_facet | Svetlana Rodimova Nikolai Bobrov Artem Mozherov Vadim Elagin Maria Karabut Polina Ermakova Ilya Shchechkin Dmitry Kozlov Dmitry Krylov Alena Gavrina Aleksandra Kashina Vladimir Zagainov Elena Zagaynova Daria Kuznetsova |
| author_sort | Svetlana Rodimova |
| collection | DOAJ |
| description | A decrease in the regenerative potential of the liver during the development of non-alcoholic fatty liver disease (NAFLD), which is observed in the vast majority of patients with diabetes mellitus type 1, significantly increases the risk of postoperative liver failure. In this regard, it is necessary to develop new approaches for the rapid intraoperative assessment of the condition of liver tissue in the presence of concomitant liver pathology. A modern label-free approach based on multiphoton microscopy, second harmonic generation (SHG), and fluorescence lifetime imaging microscopy (FLIM) allow for the evaluation of the structure of liver tissue as well as the assessment of the metabolic state of hepatocytes, even at the cellular level. We obtained optical criteria and identified specific changes in the metabolic state of hepatocytes for a reduced liver regenerative potential in the presence of induced diabetes mellitus type 1. The obtained criteria will expand the possibilities for the express assessment of the structural and functional state of liver tissue in clinical practice. |
| first_indexed | 2024-03-09T01:48:39Z |
| format | Article |
| id | doaj.art-2a895bb3b1534d2f8c0e8003d064610b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:48:39Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2a895bb3b1534d2f8c0e8003d064610b2023-12-08T15:18:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231701610.3390/ijms242317016The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime ImagingSvetlana Rodimova0Nikolai Bobrov1Artem Mozherov2Vadim Elagin3Maria Karabut4Polina Ermakova5Ilya Shchechkin6Dmitry Kozlov7Dmitry Krylov8Alena Gavrina9Aleksandra Kashina10Vladimir Zagainov11Elena Zagaynova12Daria Kuznetsova13Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaThe Volga District Medical Centre of Federal Medical and Biological Agency, 14 Ilinskaya St., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaThe Volga District Medical Centre of Federal Medical and Biological Agency, 14 Ilinskaya St., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, RussiaA decrease in the regenerative potential of the liver during the development of non-alcoholic fatty liver disease (NAFLD), which is observed in the vast majority of patients with diabetes mellitus type 1, significantly increases the risk of postoperative liver failure. In this regard, it is necessary to develop new approaches for the rapid intraoperative assessment of the condition of liver tissue in the presence of concomitant liver pathology. A modern label-free approach based on multiphoton microscopy, second harmonic generation (SHG), and fluorescence lifetime imaging microscopy (FLIM) allow for the evaluation of the structure of liver tissue as well as the assessment of the metabolic state of hepatocytes, even at the cellular level. We obtained optical criteria and identified specific changes in the metabolic state of hepatocytes for a reduced liver regenerative potential in the presence of induced diabetes mellitus type 1. The obtained criteria will expand the possibilities for the express assessment of the structural and functional state of liver tissue in clinical practice.https://www.mdpi.com/1422-0067/24/23/17016multiphoton microscopyFLIMSHGliver regenerationdiabetes mellitus |
| spellingShingle | Svetlana Rodimova Nikolai Bobrov Artem Mozherov Vadim Elagin Maria Karabut Polina Ermakova Ilya Shchechkin Dmitry Kozlov Dmitry Krylov Alena Gavrina Aleksandra Kashina Vladimir Zagainov Elena Zagaynova Daria Kuznetsova The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging International Journal of Molecular Sciences multiphoton microscopy FLIM SHG liver regeneration diabetes mellitus |
| title | The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging |
| title_full | The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging |
| title_fullStr | The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging |
| title_full_unstemmed | The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging |
| title_short | The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging |
| title_sort | effect of diabetes mellitus type 1 on the energy metabolism of hepatocytes multiphoton microscopy and fluorescence lifetime imaging |
| topic | multiphoton microscopy FLIM SHG liver regeneration diabetes mellitus |
| url | https://www.mdpi.com/1422-0067/24/23/17016 |
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