Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/21/3801 |
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| author | Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali |
| author_facet | Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali |
| author_sort | Sherif M. El-Kadi |
| collection | DOAJ |
| description | Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were <i>Bacillus paramycoides</i> MCCC 1A04098, <i>Azotobacter salinestris</i> NBRC 102611 and <i>Brevundimonas naejangsanensis</i> BIO-TAS2-2. The addition of sugar beet molasses to the medium of <i>A. salinestris</i> increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from <i>B. paramycoides</i> and <i>A. salinestris</i> showed similar bands which confirmed the presence of PHB; however, <i>B. naejangsanensis</i> showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for <i>B. paramycoides</i> and isopropyl ester of 2-butenoic acid for both of <i>A. salinestris</i> and <i>Brevundimonas naejangsanensis</i>. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics. |
| first_indexed | 2024-03-10T05:54:37Z |
| format | Article |
| id | doaj.art-2a8b7c7448754c699e5322bef41d8a1c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T05:54:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-2a8b7c7448754c699e5322bef41d8a1c2023-11-22T21:28:55ZengMDPI AGPolymers2073-43602021-11-011321380110.3390/polym13213801Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon SourcesSherif M. El-Kadi0Mohssen Elbagory1Hassan A. H. EL-Zawawy2Hossam F. A. EL-Shaer3Adel A. Shoukry4Sahar El-Nahrawy5Alaa El-Dein Omara6Dina Fathi Ismail Ali7Agricultural Microbiology Department, Faculty of Agriculture, Damietta University, Damietta 22052, EgyptDepartment of Biology, Faculty of Science and Arts, King Khalid University, Mohail Assir 61321, Saudi ArabiaBotany Department (Microbiology), Faculty of Agriculture, Al-Azhar University, Cairo 11651, EgyptBotany Department (Genetics), Faculty of Agriculture, Al-Azhar University, Cairo 11651, EgyptBotany Department (Microbiology), Faculty of Agriculture, Al-Azhar University, Cairo 11651, EgyptDepartment of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12112, EgyptDepartment of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12112, EgyptAgricultural Microbiology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, EgyptThirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were <i>Bacillus paramycoides</i> MCCC 1A04098, <i>Azotobacter salinestris</i> NBRC 102611 and <i>Brevundimonas naejangsanensis</i> BIO-TAS2-2. The addition of sugar beet molasses to the medium of <i>A. salinestris</i> increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from <i>B. paramycoides</i> and <i>A. salinestris</i> showed similar bands which confirmed the presence of PHB; however, <i>B. naejangsanensis</i> showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for <i>B. paramycoides</i> and isopropyl ester of 2-butenoic acid for both of <i>A. salinestris</i> and <i>Brevundimonas naejangsanensis</i>. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.https://www.mdpi.com/2073-4360/13/21/3801agro-industrial wasteGC-MS/MS spectrometryIR spectrometryPHB |
| spellingShingle | Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources Polymers agro-industrial waste GC-MS/MS spectrometry IR spectrometry PHB |
| title | Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources |
| title_full | Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources |
| title_fullStr | Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources |
| title_full_unstemmed | Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources |
| title_short | Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources |
| title_sort | biosynthesis of poly ss hydroxybutyrate phb from different bacterial strains grown on alternative cheap carbon sources |
| topic | agro-industrial waste GC-MS/MS spectrometry IR spectrometry PHB |
| url | https://www.mdpi.com/2073-4360/13/21/3801 |
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