Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere
IntroductionPhytobiomes have a significant impact on plant health. The microbiome of Cannabis sativa is particularly interesting both because of renewed interest in this crop and because it is commercially propagated in two different ways (i.e. clonally and by seed). Angiosperms obtain a founding po...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326294/full |
| _version_ | 1797301439584796672 |
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| author | Christopher R. Dumigan Michael K. Deyholos |
| author_facet | Christopher R. Dumigan Michael K. Deyholos |
| author_sort | Christopher R. Dumigan |
| collection | DOAJ |
| description | IntroductionPhytobiomes have a significant impact on plant health. The microbiome of Cannabis sativa is particularly interesting both because of renewed interest in this crop and because it is commercially propagated in two different ways (i.e. clonally and by seed). Angiosperms obtain a founding population of seed-borne endophytes from their seed-bearing parent. This study examines the influence of both seed and soil-derived bacteria on the endospheres of cannabis seedlings of both hemp- and drug-types.MethodsA multi-factorial metagenomic study was conducted with three cannabis genotypes and two soil sources, which were tested both before and after autoclave sterilization. Seedlings were grown on soil, then rinsed and surface-sterilized, and 16S rDNA amplicons from seedling endophytes were sequenced, taxonomically classified, and used to estimate alpha- and beta-diversity in Qiime2. The statistical significance of differences in seedling microbiomes across treatments was tested, and PiCRUST2 was used to infer the functional relevance of these differences.ResultsSoil was found to have a profound effect on the alpha-diversity, beta-diversity, relative abundance, and functional genes of endophytic bacteria in germinating cannabis seedlings. Additionally, there was a significant effect of cannabis genotype on beta diversity, especially when genotypes were grown in sterilized soil. Gammaproteobacteria and Bacilli were the two most abundant taxa and were found in all genotypes and soil types, including sterilized soil.DiscussionThe results indicated that a component of cannabis seedling endosphere microbiomes is seed-derived and conserved across the environments tested. Functional prediction of seedling endophytes using piCRUST suggested a number of important functions of seed-borne endophytes in cannabis including nutrient and amino acid cycling, hormone regulation, and as precursors to antibiotics. This study suggested both seed and soil play a critical role in shaping the microbiome of germinating cannabis seedlings. |
| first_indexed | 2024-03-07T23:21:36Z |
| format | Article |
| id | doaj.art-2a8d8c04f2b644209b0dc4a1d2661687 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-07T23:21:36Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2a8d8c04f2b644209b0dc4a1d26616872024-02-21T05:28:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13262941326294Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphereChristopher R. DumiganMichael K. DeyholosIntroductionPhytobiomes have a significant impact on plant health. The microbiome of Cannabis sativa is particularly interesting both because of renewed interest in this crop and because it is commercially propagated in two different ways (i.e. clonally and by seed). Angiosperms obtain a founding population of seed-borne endophytes from their seed-bearing parent. This study examines the influence of both seed and soil-derived bacteria on the endospheres of cannabis seedlings of both hemp- and drug-types.MethodsA multi-factorial metagenomic study was conducted with three cannabis genotypes and two soil sources, which were tested both before and after autoclave sterilization. Seedlings were grown on soil, then rinsed and surface-sterilized, and 16S rDNA amplicons from seedling endophytes were sequenced, taxonomically classified, and used to estimate alpha- and beta-diversity in Qiime2. The statistical significance of differences in seedling microbiomes across treatments was tested, and PiCRUST2 was used to infer the functional relevance of these differences.ResultsSoil was found to have a profound effect on the alpha-diversity, beta-diversity, relative abundance, and functional genes of endophytic bacteria in germinating cannabis seedlings. Additionally, there was a significant effect of cannabis genotype on beta diversity, especially when genotypes were grown in sterilized soil. Gammaproteobacteria and Bacilli were the two most abundant taxa and were found in all genotypes and soil types, including sterilized soil.DiscussionThe results indicated that a component of cannabis seedling endosphere microbiomes is seed-derived and conserved across the environments tested. Functional prediction of seedling endophytes using piCRUST suggested a number of important functions of seed-borne endophytes in cannabis including nutrient and amino acid cycling, hormone regulation, and as precursors to antibiotics. This study suggested both seed and soil play a critical role in shaping the microbiome of germinating cannabis seedlings.https://www.frontiersin.org/articles/10.3389/fpls.2024.1326294/fullmicrobiotahempendophytemetagenomephytobiome |
| spellingShingle | Christopher R. Dumigan Michael K. Deyholos Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere Frontiers in Plant Science microbiota hemp endophyte metagenome phytobiome |
| title | Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere |
| title_full | Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere |
| title_fullStr | Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere |
| title_full_unstemmed | Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere |
| title_short | Soil and seed both influence bacterial diversity in the microbiome of the Cannabis sativa seedling endosphere |
| title_sort | soil and seed both influence bacterial diversity in the microbiome of the cannabis sativa seedling endosphere |
| topic | microbiota hemp endophyte metagenome phytobiome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326294/full |
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