TaxAss: Leveraging a Custom Freshwater Database Achieves Fine-Scale Taxonomic Resolution

TaxAss: Leveraging a Custom Freshwater Database Achieves Fine-Scale Taxonomic Resolution

ABSTRACT Taxonomy assignment of freshwater microbial communities is limited by the minimally curated phylogenies used for large taxonomy databases. Here we introduce TaxAss, a taxonomy assignment workflow that classifies 16S rRNA gene amplicon data using two taxonomy reference databases: a large com...

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Bibliographic Details
Main Authors: Robin R. Rohwer, Joshua J. Hamilton, Ryan J. Newton, Katherine D. McMahon
Format: Article
Language:English
Published: American Society for Microbiology 2018-10-01
Series:mSphere
Subjects:
16S rRNA gene
amplicon sequencing
freshwater
limnology
microbial ecology
taxonomy
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00327-18

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