Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium
Abstract Background This study observed and described the morphological characteristics of the endometrium of the resected uterus using confocal laser endomicroscopy. This included benign endometrium, non-atypical endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-022-10137-x |
| _version_ | 1828164066020950016 |
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| author | Jia Wen Xi Yang Guiying Ye Rui Chen Yu Feng Qinping Liao |
| author_facet | Jia Wen Xi Yang Guiying Ye Rui Chen Yu Feng Qinping Liao |
| author_sort | Jia Wen |
| collection | DOAJ |
| description | Abstract Background This study observed and described the morphological characteristics of the endometrium of the resected uterus using confocal laser endomicroscopy. This included benign endometrium, non-atypical endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma, thereby laying a foundation for finding the precise localization and resection of endometrial lesions, given the feasibility of confocal laser endomicroscopy-assisted hysteroscopy. Methods This prospective study included 32 patients who underwent hysterectomy. We used confocal laser endomicroscopy to observe the endometrium of resected uteruses and described the characteristics of endometrium in different states by comparing histopathological findings (primary objects). The secondary objects of observation were the myometrium, endocervical canal, and surface of the external os of the cervix. Results A total of 32 patients who underwent hysterectomy for different diseases were included: 9 with endometrial carcinoma (5 with endometrioid carcinoma, 1 with endometrial serous carcinoma, 2 with clear cell carcinoma, and 1 with carcinosarcoma), 2 with atypical endometrial hyperplasia, 9 with benign diseases, 7 with cervical cancer, and 5 with ovarian cancer and borderline tumor. The dynamic images of the endometrium were observed and recorded using probe-based confocal laser endomicroscopy (pCLE). Considering histopathology as the gold standard, the diagnostic concordance rate of pCLE was 96.9% in patients with endometrial carcinoma and precancerous lesions and 100% in patients with endometrial carcinoma. Conclusion Confocal laser endomicroscopy provides real-time high-resolution images of the benign endometrium and endometrial lesions. Compared with histopathology, confocal laser endomicroscopy has high diagnostic accuracy and may become an auxiliary examination tool for hysteroscopy, as it is useful for early identification of endometrial lesions, real-time diagnosis of tumor, and detection of tumor boundaries for complete tumor resection. These findings can lay a foundation for the feasible use of fertility-sparing local excision of tumor lesions by hysteroscopy. |
| first_indexed | 2024-04-12T01:20:40Z |
| format | Article |
| id | doaj.art-2a8e84459d4b4ee9bf4070e2d272d605 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-12T01:20:40Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-2a8e84459d4b4ee9bf4070e2d272d6052022-12-22T03:53:48ZengBMCBMC Cancer1471-24072022-10-0122111110.1186/s12885-022-10137-xPreliminary study of confocal laser endomicroscopy for in vitro specimens of the endometriumJia Wen0Xi Yang1Guiying Ye2Rui Chen3Yu Feng4Qinping Liao5Department of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua UniversityDepartment of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua UniversityDepartment of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua UniversityDepartment of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua UniversityDepartment of Biomedical Engineering, Tsinghua University School of MedicineDepartment of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua UniversityAbstract Background This study observed and described the morphological characteristics of the endometrium of the resected uterus using confocal laser endomicroscopy. This included benign endometrium, non-atypical endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma, thereby laying a foundation for finding the precise localization and resection of endometrial lesions, given the feasibility of confocal laser endomicroscopy-assisted hysteroscopy. Methods This prospective study included 32 patients who underwent hysterectomy. We used confocal laser endomicroscopy to observe the endometrium of resected uteruses and described the characteristics of endometrium in different states by comparing histopathological findings (primary objects). The secondary objects of observation were the myometrium, endocervical canal, and surface of the external os of the cervix. Results A total of 32 patients who underwent hysterectomy for different diseases were included: 9 with endometrial carcinoma (5 with endometrioid carcinoma, 1 with endometrial serous carcinoma, 2 with clear cell carcinoma, and 1 with carcinosarcoma), 2 with atypical endometrial hyperplasia, 9 with benign diseases, 7 with cervical cancer, and 5 with ovarian cancer and borderline tumor. The dynamic images of the endometrium were observed and recorded using probe-based confocal laser endomicroscopy (pCLE). Considering histopathology as the gold standard, the diagnostic concordance rate of pCLE was 96.9% in patients with endometrial carcinoma and precancerous lesions and 100% in patients with endometrial carcinoma. Conclusion Confocal laser endomicroscopy provides real-time high-resolution images of the benign endometrium and endometrial lesions. Compared with histopathology, confocal laser endomicroscopy has high diagnostic accuracy and may become an auxiliary examination tool for hysteroscopy, as it is useful for early identification of endometrial lesions, real-time diagnosis of tumor, and detection of tumor boundaries for complete tumor resection. These findings can lay a foundation for the feasible use of fertility-sparing local excision of tumor lesions by hysteroscopy.https://doi.org/10.1186/s12885-022-10137-xOrgan preservationFertility sparing/preservationOptical biopsyConfocal laser endomicroscopyEndometrial carcinomaAtypical endometrial hyperplasia |
| spellingShingle | Jia Wen Xi Yang Guiying Ye Rui Chen Yu Feng Qinping Liao Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium BMC Cancer Organ preservation Fertility sparing/preservation Optical biopsy Confocal laser endomicroscopy Endometrial carcinoma Atypical endometrial hyperplasia |
| title | Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| title_full | Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| title_fullStr | Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| title_full_unstemmed | Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| title_short | Preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| title_sort | preliminary study of confocal laser endomicroscopy for in vitro specimens of the endometrium |
| topic | Organ preservation Fertility sparing/preservation Optical biopsy Confocal laser endomicroscopy Endometrial carcinoma Atypical endometrial hyperplasia |
| url | https://doi.org/10.1186/s12885-022-10137-x |
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