Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids
The culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organism...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2009-07-01
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| Series: | Journal of Laboratory Physicians |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.59703 |
| _version_ | 1819016308156530688 |
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| author | Nita Pal Rajni Sharma Suman Rishi Leela Vyas |
| author_facet | Nita Pal Rajni Sharma Suman Rishi Leela Vyas |
| author_sort | Nita Pal |
| collection | DOAJ |
| description | The culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organisms. The specimens were processed by automated BACTEC 9120 culture system. A total of 1,677 clinically significant microorganisms were isolated. Gram positive and negative bacterial isolation rates were found to be 62.55% and 32.20%, respectively. Yeasts were recovered in 5.24%. False positivity rate was 1.5%. Clinically significant isolates recovered on day four and five were 97.81% and 99.88%, respectively. At day five, the sensitivity was 99.94% and negative predictive value 99.96%. Our data support a five-day incubation protocol for recovery of all clinically significant organisms with sensitivity reduced by 0.06%, when compared with a six-day protocol. |
| first_indexed | 2024-12-21T02:45:34Z |
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| id | doaj.art-2a8ed0ab53fb4675a3c2ead238021f70 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-21T02:45:34Z |
| publishDate | 2009-07-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-2a8ed0ab53fb4675a3c2ead238021f702022-12-21T19:18:34ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262009-07-0110206907210.4103/0974-2727.59703Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body FluidsNita Pal0Rajni Sharma1Suman Rishi2Leela Vyas3Department of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaDepartment of Microbiology, SMS Medical College, Jaipur, IndiaThe culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organisms. The specimens were processed by automated BACTEC 9120 culture system. A total of 1,677 clinically significant microorganisms were isolated. Gram positive and negative bacterial isolation rates were found to be 62.55% and 32.20%, respectively. Yeasts were recovered in 5.24%. False positivity rate was 1.5%. Clinically significant isolates recovered on day four and five were 97.81% and 99.88%, respectively. At day five, the sensitivity was 99.94% and negative predictive value 99.96%. Our data support a five-day incubation protocol for recovery of all clinically significant organisms with sensitivity reduced by 0.06%, when compared with a six-day protocol.http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.59703automated blood culture systembactectime to detection |
| spellingShingle | Nita Pal Rajni Sharma Suman Rishi Leela Vyas Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids Journal of Laboratory Physicians automated blood culture system bactec time to detection |
| title | Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids |
| title_full | Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids |
| title_fullStr | Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids |
| title_full_unstemmed | Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids |
| title_short | Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body Fluids |
| title_sort | optimum time to detection of bacteria and yeast species with bactec 9120 culture system from blood and sterile body fluids |
| topic | automated blood culture system bactec time to detection |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.59703 |
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