Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase
Hypochlorous acid (HClO) is a common reactive oxygen species (ROS), with a high chemical reactivity. Myeloperoxidase (MPO) is an enzyme that catalyzes in vivo redox reactions between H<sub>2</sub>O<sub>2</sub> and Cl<sup>−</sup> to produce HClO. Abnormal levels of...
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MDPI AG
2023-06-01
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| Series: | Chemistry |
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| Online Access: | https://www.mdpi.com/2624-8549/5/2/98 |
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| author | Bo Liu Jia-Yi Ma Jing Wang Dong-Xia Wang An-Na Tang De-Ming Kong |
| author_facet | Bo Liu Jia-Yi Ma Jing Wang Dong-Xia Wang An-Na Tang De-Ming Kong |
| author_sort | Bo Liu |
| collection | DOAJ |
| description | Hypochlorous acid (HClO) is a common reactive oxygen species (ROS), with a high chemical reactivity. Myeloperoxidase (MPO) is an enzyme that catalyzes in vivo redox reactions between H<sub>2</sub>O<sub>2</sub> and Cl<sup>−</sup> to produce HClO. Abnormal levels of HClO and MPO may lead to oxidative stress, irreversible tissue damage and, thus, serious diseases; they are thus becoming important biomarkers and therapeutic targets. In this work, using HClO-induced site-specific cleavage of phosphorothioate-modified DNA to trigger rolling circle amplification (RCA), RCA-assisted biosensors have been developed for the highly sensitive and specific detection of HClO and MPO. Only two DNA oligonucleotides are used in the sensing systems. The powerful signal-amplification capability of RCA endows the sensing systems with a high sensitivity, and the specific fluorescent response of thioflavin T (ThT) to G-quadruplexes in RCA products makes a label-free signal output possible. The proposed biosensors were demonstrated to work well not only for the sensitive and specific quantitation of HClO and MPO with detection limits of 1.67 nM and 0.33 ng/mL, respectively, but also for the screening and inhibitory capacity evaluation of MPO inhibitors, thus holding great promise in disease diagnosis and drug analysis. |
| first_indexed | 2024-03-11T02:38:33Z |
| format | Article |
| id | doaj.art-2a927adcd1e345c4b65e57c539b895bc |
| institution | Directory Open Access Journal |
| issn | 2624-8549 |
| language | English |
| last_indexed | 2024-03-11T02:38:33Z |
| publishDate | 2023-06-01 |
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| series | Chemistry |
| spelling | doaj.art-2a927adcd1e345c4b65e57c539b895bc2023-11-18T09:45:52ZengMDPI AGChemistry2624-85492023-06-01521454146410.3390/chemistry5020098Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and MyeloperoxidaseBo Liu0Jia-Yi Ma1Jing Wang2Dong-Xia Wang3An-Na Tang4De-Ming Kong5State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, ChinaSchool of Medical Laboratory, College of Medical Technology, Tianjin Medical University, Guangdong Road, Tianjin 300203, ChinaState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, ChinaHypochlorous acid (HClO) is a common reactive oxygen species (ROS), with a high chemical reactivity. Myeloperoxidase (MPO) is an enzyme that catalyzes in vivo redox reactions between H<sub>2</sub>O<sub>2</sub> and Cl<sup>−</sup> to produce HClO. Abnormal levels of HClO and MPO may lead to oxidative stress, irreversible tissue damage and, thus, serious diseases; they are thus becoming important biomarkers and therapeutic targets. In this work, using HClO-induced site-specific cleavage of phosphorothioate-modified DNA to trigger rolling circle amplification (RCA), RCA-assisted biosensors have been developed for the highly sensitive and specific detection of HClO and MPO. Only two DNA oligonucleotides are used in the sensing systems. The powerful signal-amplification capability of RCA endows the sensing systems with a high sensitivity, and the specific fluorescent response of thioflavin T (ThT) to G-quadruplexes in RCA products makes a label-free signal output possible. The proposed biosensors were demonstrated to work well not only for the sensitive and specific quantitation of HClO and MPO with detection limits of 1.67 nM and 0.33 ng/mL, respectively, but also for the screening and inhibitory capacity evaluation of MPO inhibitors, thus holding great promise in disease diagnosis and drug analysis.https://www.mdpi.com/2624-8549/5/2/98hypochlorous acidmyeloperoxidaserolling circle amplificationphosphorothioatebiosensor |
| spellingShingle | Bo Liu Jia-Yi Ma Jing Wang Dong-Xia Wang An-Na Tang De-Ming Kong Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase Chemistry hypochlorous acid myeloperoxidase rolling circle amplification phosphorothioate biosensor |
| title | Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase |
| title_full | Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase |
| title_fullStr | Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase |
| title_full_unstemmed | Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase |
| title_short | Rolling-Circle-Amplification-Assisted DNA Biosensors for Sensitive and Specific Detection of Hypochlorous Acid and Myeloperoxidase |
| title_sort | rolling circle amplification assisted dna biosensors for sensitive and specific detection of hypochlorous acid and myeloperoxidase |
| topic | hypochlorous acid myeloperoxidase rolling circle amplification phosphorothioate biosensor |
| url | https://www.mdpi.com/2624-8549/5/2/98 |
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